甲醇、戊二醛交联剂对丝素蛋白/明胶多孔支架的性能影响

为了对比甲醇、戊二醛两种交联剂对丝素蛋白/明胶复合多孔支架的性能影响,采用冷冻干燥法等比例制备该支架,分两组分别用甲醇和戊二醛进行交联。通过观察支架的微观形貌,测量支架的孔隙率、吸水率、溶胀率,测试热稳定性及力学性能,比较经两种交联剂处理后支架结构和性能的变化。结果表明,经戊二醛交联后的支架孔隙分布更加规则、均匀,孔隙率、吸水率、溶胀率更高,力学性能更强。采用戊二醛交联丝素蛋白/明胶复合多孔支架,能够使支架性能更加优良。     

Investigation of cancer cell behavior on nanofibrous scaffolds

Tissue engineering and the use of nanofibrous biomaterial scaffolds offer a unique perspective for studying cancer development in vitro. Current in vitro models of tumorigenesis are limited by the use of static, two-dimensional (2D) cell culture monolayers that lack the structural architecture necessary for cell-cell interaction and three-dimensional (3D) scaffolds that are too simplistic for studying basic pathological mechanisms. In this study, two nanofibrous biomaterials that mimic the structure of the extracellular matrix, bacterial cellulose and electrospun polycaprolactone (PCL)/collagen I, were investigated as potential 3D scaffolds for an in vitro cancer model. Multiple cancer cell lines were cultured on each scaffold material and monitored for cell viability, proliferation, adhesion, infiltration, and morphology. Both bacterial cellulose and electrospun PCL/collagen I, which have nano-scale structures on the order of 100-500 nm, have been used in many diverse tissue engineering applications. Cancer cell adhesion and growth were limited on bacterial cellulose, while all cellular processes were enhanced on the electrospun scaffolds. This initial analysis has demonstrated the potential of electrospun PCL/collagen I scaffolds toward the development of an improved 3D in vitro cancer model.     

多级开孔壳聚糖海绵的细胞行为分析

使用冰滴为致孔剂制备表面大孔、内部孔洞相连的新型壳聚糖(HPCS)支架,将其与聚乳酸复合制备出三维蜂窝状孔洞结构的复合支架(THCP).对HPCS和THCP进行了表面形貌、力学性能、细胞相容性等方面的表征,并与常规冻干法所制备的壳聚糖(CS)海绵进行了对比.结果表明,在HPCS海绵表面均匀分布着大而开放的孔洞,大孔内部...     

Injectable Collagen–Genipin Gel for the Treatment of Spinal Cord Injury: In Vitro Studies

Spinal cord injury (SCI) often results in a cavitary lesion, contained within the dura, which involves only a portion of the cord. Injectable biopolymers are an attractive treatment option for SCI to re‐establish cell migratory pathways within the lesion while minimizing the collateral damage attendant to an open surgical procedure. In this study we evaluate a thermoresponsive soluble collagen gel incorporating genipin, an amine reactive covalent cross‐linker with low cytotoxicity and fluorogenic attributes. Unlike previous studies, genipin is being investigated as an in situ covalent cross‐linker that will continue to act on the gel after injection. Physical characterization studies show that the addition of genipin provides control over the mechanical and degradative behavior of the gel, to meet design specifications of an injectable material for neural tissues. Additionally, an improved in situ assay to predict the extent of cross‐linking reaction is investigated. Encapsulation of mesenchymal stem cells (MSCs) in collagen–genipin gels show the gels support cell viability and proliferation, and thus serve as a cell delivery vehicle. Neural stem cells are found to be more sensitive to genipin, with respect to toxicity, as compared to MSCs. From our studies, 0.25‐0.5 mM is an appropriate genipin concentration to use for an in situ forming scaffold capable of delivering cells and therapeutic agents.     

碗扣式支架在现浇连续箱梁桥施工中的应用

介绍了碗扣式支架在跨线桥连续箱梁施工中的应用概况、施工工艺和控制要点,通过工程实例,详细论述了碗扣式支架的平面布置及稳定性验算方法,形成了系统地现浇连续箱梁桥碗扣式支架施工方案,对同类工程具有一定的指导意义。     

某特大桥连续梁0号块现浇膺架法施工技术

从支架搭设,模板安装,支架预压等方面入手,详细介绍了某特大桥连续梁0号块现浇膺架法施工技术,着重阐述了对该桥的力学检算过程,并提出了施工应注意事项,为今后同类桥梁现浇膺架法施工积累了一定经验。     

基于某水利工程模板设计与施工措施探讨

对某水利工程模板设计及施工进行了探讨,根据整体提升模板施工程序,分别阐述了整体提升模板各分部工序施工工艺及操作要点,并介绍了相应的施工安全质量保证措施,为今后同类模板工程积累了一定经验。     

高空大跨度悬挑脚手架在某高层住宅施工中的应用

本文以某高层住宅项目为例,重点对高空大跨度悬挑脚手架的设计与施工进行介绍。本项目采用吊拉式悬挑型钢承力架作为悬挑脚手架的支承架,通过计算分析,并考虑各节点的构造措施,设计出完整的悬挑脚手架方案,在工程中得到良好的应用。本文还对比两种计算模型的内力与挠度计算结果,说明对钢丝绳采取的不恰当收紧措施会导致悬挑承力架的内力和挠度发生显著的变化,实际工程中应予避免。     

脚手架稳定承载力影响因素的有限元分析

采用ANSYS软件对扣件式钢管模板支架进行了有限元模拟,分析了考虑扣件式节点为刚接节点、铰接节点和半刚性节点对模板支架特征值屈曲承载力的影响;并分析了立杆步距、立杆间距、剪刀撑等因素对支架特征值承载力的影响。