安钢高炉高风温生产实践

针对安钢炼铁厂高炉风温低,能耗高的情况,生产中采取了改造热风炉系统,引进新技术,提高废气温度,改善原燃料条件,稳定高炉操作等措施,风温水平逐步提高,2005年全厂平均风温达到1083℃,取得了明显效果.     

Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer

The clonal composition of a malignant tumor strongly depends on cellular dynamics influenced by the asynchronized loss of DNA repair mechanisms. Here, our aim was to identify founder mutations leading to subsequent boosts in mutation load. The overall mutation burden in 591 colorectal cancer tumors was analyzed, including the mutation status of DNA-repair genes. The number of mutations was first determined across all patients and the proportion of genes having mutation in each percentile was ranked. Early mutations in DNA repair genes preceding a mutational expansion were designated as founder mutations. Survival analysis for gene expression was performed using microarray data with available relapse-free survival. Of the 180 genes involved in DNA repair, the top five founder mutations were in PRKDC (n = 31), ATM (n = 26), POLE (n = 18), SRCAP (n = 18), and BRCA2 (n = 15). PRKDC expression was 6.4-fold higher in tumors compared to normal samples, and higher expression led to longer relapse-free survival in 1211 patients (HR = 0.72, p = 4.4 × 10⁻³). In an experimental setting, the mutational load resulting from UV radiation combined with inhibition of PRKDC was analyzed. Upon treatments, the mutational load exposed a significant two-fold increase. Our results suggest PRKDC as a new key gene driving tumor heterogeneity.     

不锈钢酸洗污泥对烧结黏结相与平衡相的影响

 不锈钢酸洗污泥安全填埋占用土地且难以消除对环境的危害,将酸洗污泥作为烧结配料使用有望回收其中铁、铬、镍等金属,并充分利用其中的熔剂组分,实现酸洗污泥在冶金企业的闭路资源化利用。铁矿粉配加酸洗污泥烧结黏结相强度试验表明,随着酸洗污泥配加比例的增加,黏结相强度先增加后减小,且随着碱度的增加整体减小,当酸洗污泥配加比例为10%时,试样中赤铁矿、磁铁矿质量分数最大,枪晶石质量分数较小,在碱度为1.5时于1 280 ℃焙烧4 min,试样的黏结相强度最大可达15.12 kN。平衡相烧结试验表明,配加酸洗污泥能改善烧结矿质量,铁矿粉配加10%的酸洗污泥,碱度为1.5,1 300 ℃烧结240 min后,烧结矿的矿相组成和微观结构最好,综合性能最佳。     

鞍钢提高高炉利用系数生产管理实践

针对鞍钢股份有限公司炼铁总厂高炉群平均利用系数低的问题,从原料条件、操作炉型和操作管理三个方面进行了原因分析,通过采取原燃料指标量化管理、高炉炉型诊断管理、操作参数科学管理等措施,克服了原燃料结构变化等不利因素,提高了高炉群平均利用系数,实现了高炉长周期稳定顺行。     

再生纺涤纶短纤维优化配料模型研究

根据约束线性规划原理及涤纶短纤维生产过程中物理变化的内在联系,建立了中空涤纶短纤维配料的数学模型,该模型充分考虑了再生涤纶短纤维生产的工艺要求,得到了适合生产需要的配料结果。通过实例模拟配料计算,该模型比常规配料计算更科学。     

安钢高炉炉料结构优化研究及生产实践

在目前原燃料条件下,结合安钢炼铁生产实际,以铁前系统经济技术指标改善和降本增效为目的,对安钢高炉炉料结构进行了优化及试验研究,并在高炉生产中进行了初步工业应用.生产实践表明,目前在安钢铁前原燃料条件及现有工艺装备条件下,安钢高炉炉料结构以高碱度烧结矿(R=2.0左右)为主体(65%～70%),同时配加酸性球团矿(20%～25%左右)及少量生块矿(8%～12%)是较为经济合理的、可行的.     

安钢6号高炉降低焦比的技术攻关

对安钢6号高炉降低焦比的技术攻关进行了总结.通过分析影响因素,更新操作理念,采取改善入炉原燃料质量;大矿批、全正装、灵活布料、高风温、高煤比、高氧量;合适的低[S]〗低[S]冶炼;强化出铁出渣管理等措施,使6号高炉在2007年3月焦比达到358kg/t,比去年同期降低45kg/t.     

Understanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach

The cellular changes induced by heterologous protein expression in the yeast Saccharomyces cerevisiae have been analysed on many levels and found to be significant. However, even though high‐level protein production poses a metabolic burden, evaluation of the expression host at the level of the metabolome has often been neglected. We present a comparison of metabolite profiles of a wild‐type strain with those of three strains producing recombinant antibody variants of increasing size and complexity: an scFv fragment, an scFv–Fc fusion protein and a full‐length IgG molecule. Under producing conditions, all three recombinant strains showed a clear decrease in growth rate compared with the wild‐type strain and the severity of the growth phenotype increased with size of the protein. The levels of 76 intracellular metabolites were determined using a targeted (semi) quantitative mass spectrometry based approach. Based on unsupervised and supervised multivariate analysis of metabolite profiles, together with pathway activity profiling, the recombinant strains were found to be significantly different from each other and from the wild‐type strain. We observed the most prominent changes in metabolite levels for metabolites involved in amino acid and redox metabolism. Induction of the unfolded protein response was detected in all producing strains and is considered to be a contributing factor to the overall metabolic burden on the cells.     

化学交换精馏法分离硼同位素分解反应实验研究

三氟化硼-苯甲醚的分解反应是三氟化硼-苯甲醚络合物化学交换精馏法分离硼同位素不可缺少的步骤。由于三氟化硼-苯甲醚络合物分解过程中伴随苯甲醚的分解,使过程变得复杂,文中理论分析了苯甲醚分解的原因,用逆流多级串联装置研究了分解反应,并用高效液相色谱对产物进行分析;为获得反应的最佳操作条件,测定了反应时间和温度对络合物分解反应的影响,并分析了产生这种结果的原因。结果表明,当反应时间为0.5—1 h、温度为150℃时产物中苯甲醚的质量分数可达到97%以上,从而使络合物完全分解而苯甲醚最小程度的分解,确保了整个分离系统的正常连续运转。