Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency

Excessive input of nitrogen fertilizer not only causes a great waste of resources but brings about a series of ecological and environmental problems. Although Small Auxin Up-regulated RNAs (SAURs) participate in diverse biological processes, the function of SAURs in the nitrogen starvation response has not been well-studied. Here, we identified 308 TaSAURs in wheat and divided them into 10 subfamilies. The promoter regions of most TaSAURs contain hormone responsive elements, and their expression levels change under the treatment of different hormones, such as IAA, MeJA, and ABA. Interestingly, overexpression of one of the TaSAUR family members, a nitrogen starvation responsive gene, TaSAUR66-5B, can promote the growth of Arabidopsis and wheat roots. In addition, overexpression of TaSAUR66-5B in Arabidopsis up-regulates the expression levels of auxin biosynthesis related genes, suggesting that overexpression TaSAUR66-5B may promote root growth by increasing the biosynthesis of auxin. Furthermore, overexpression of TaSAUR66-5B in wheat can increase the biomass and grain yields of transgenic plants, as well as the nitrogen concentration and accumulation of both shoots and grains, especially under low nitrogen conditions. This study provides important genomic information of the TaSAUR gene family and lays a foundation for elucidating the functions of TaSAURs in improving nitrogen utilization efficiency in wheat.     

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Colibacillosis is an acute and chronic avian disease caused by avian pathogenic E. coli (APEC). Previous studies have demonstrated that RIP2 plays a significant role in APEC infection. Moreover, increasing evidence indicates that microRNAs (miRNAs) are involved in host–pathogen interactions and the immune response. However, the role of miRNAs in the host against APEC infection remains unclear. Herein, we attempted to reveal new miRNAs potentially involved in the regulation of the immune and inflammatory response against APEC infection, with a particular focus on those possibly correlated with RIP2 expression, via miRNA-seq, RT-qPCR, Western blotting, dual-luciferase reporter assay, and CCK-8. The results showed that a total of 93 and 148 differentially expressed (DE) miRNAs were identified in the knockdown of RIP2 cells following APEC infection (shRIP2+APEC) vs. knockdown of RIP2 cells (shRIP2) and shRIP2 vs. wild-type cells (WT), respectively. Among those identified DE miRNAs, the biological function of gga-miR-455-5p was investigated. It was found that gga-miR-455-5p regulated by RIP2 was involved in the immune and inflammatory response against APEC infection via targeting of IRF2 to modulate the expression of type I interferons. Additionally, RIP2 could directly regulate the production of the type I interferons. Altogether, these findings highlighted the crucial role of miRNAs, especially gga-miR-455-5p, in host defense against APEC infection.     

贵溪鲍家矿业公司选别工艺改造实践

根据原矿的矿物特性,在试验研究的基础上,将原混合浮选工艺改造成优先浮选工艺,产出了合格的单一铅、锌精矿产品,从而调整了产品结构,取得了较好的选矿技术指标,公司新增经济效益明显.     

基于三次回归分析的试车台基线库的建立

试车台基线是航空发动机生产商或维修企业挑选性能理想的发动机在试车台上试车所得到的性能参数的趋势线。将维修后的发动机的试车数据曲线与相应参数的基线进行对比,就可得到该发动机维修后的性能状况。因此基线在试车结果分析中起着重要的作用。目前,工程师通常是利用Excel软件对试车数据进行处理来得到基线的,这种方法效率低,精度差,且容易出错。本文利用三次回归分析法建立了发动机试车台基线的数学模型,并对该数学模型所产生的误差进行估计,提出了基线库的建立方法。通过在发动机全寿命管理系统中的应用,证明了本文所述的模型的实用性。     

Fe_(16)N_2 的沉淀效应

含氮马氏体室温时效和低于１７５℃回火时有Ｆｅ１６Ｎ２沉淀。Ｆｅ１６Ｎ２的沉淀效应显著,于１００℃回火时沉淀效应最大,ＨＶ、ｃ／ａ比和α（２２０）半高宽均达到最大值。Ｆｅ１６Ｎ２淀淀具有很高的强化效果,纯铁和２０钢渗氮后于１００℃回火,硬度分别提高５８％和６２％。Ｆｅ１６Ｎ２的沉淀效应具有广泛的应用前景     

ZG270-500铸钢吊具断裂分析

对断裂起重机吊具ZG270-500铸钢的化学成分、硬度、显微组织、断口形貌等进行了检测和分析。结果表明,ZG270-500铸钢吊具断裂的主要原因是存在魏氏组织铁素体,并且出现了晶粒粗大和大量孔隙等铸造缺陷。通过870℃正火处理后魏氏组织得到消除,钢的冲击吸收功和硬度分别达到23.5J和170HBS,力学性能达到GB11352—1989要求。     

喷丸加工的工艺特性及参数控制研究

提出了喷丸加工新算法,该算法是模拟喷丸过程中零件表面层的塑性变化,从而控制能量参数。在喷丸加工时,表面层塑性变形积聚了大量压缩能量,通过进行能量参数优化,得到零件的最大疲劳强度并绘出曲率参数曲线。     

等通道转角挤压自增强等规聚丙烯研究

为了研究等通道转角挤压等规聚丙烯的挤压工艺条件、结构与性能之间的关系,采用90°转角模具及挤压路径A、在熔点以下的160℃制备了不同挤压次数的等规聚丙烯样品,利用广角X射线衍射(WAXD)、差示扫描量热分析(DSC)等手段对其结构进行表征.结果表明,随挤压次数增加,等规聚丙烯分子取向明显增加,材料结晶度提高,试样的力学性能提高十分显著,未挤压试样的抗拉强度为17.13 MPa,经过三次挤压后,抗拉强度提高到59.38 MPa.     

三角形PCD的质量状况及应用前景

三角形PCD的剪切破岩方式使其在塑性、塑脆性地层钻进效果较好.国产三角形PCD石油钻头难以见到应有的效果,碎断齿现象严重.通过检测国内外三角形PCD产品性能发现:国产三角形PCD的抗冲击韧性大大低于行业标准规定值;同一批产品的磨耗比数值差异较大;由于自锐性差,在测定磨耗比过程中,当其尖角磨损面较大时易出现发红现象;与国外同类产品相比有较大差距.采用以1/3的圆柱形(80°～85°锥角)PCD替代三角形PCD制作的混合齿钻头,使用效果有明显改善,但仍难以取代三角形PCD钻头在钻进某些地层的特殊作用.国产高质量三角形PCD钻头将会为提高日益增多的超深井复杂地质条件下的钻进效果发挥其应有的作用.     

LASER 300S在线激光打孔参数的选择

通过对PROTOS1-8高速卷烟机在线激光打孔参数的组合试验和数据回归分析,得出了烟支稀释率与脉冲、脉冲与能量的乘积、能量、打孔数量等参数的线性相关方程。试验结果表明,利用各线性方程可计算出能保证LASER 300S激光打孔系统正常运行的参数组合,与实际在线打孔的烟支稀释率值具有较好的符合性和稳定性,能够提高在线加工质量。