Genomic Insights of First ermB-Positive ST338-SCCmecVT/CC59 Taiwan Clone of Community-Associated Methicillin-Resistant Staphylococcus aureus in Poland

We report the first Polish representative of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA), lukS/F-PV-positive, encoding the ermB gene, as a genetic determinant of constitutive resistance to macrolides, lincosamides, and streptogramin B antibiotics, cMLS-B. This is the first detection of the CA-MRSA strain responsible for nosocomial infection in the Warsaw Clinical Hospital. Resistance to β-lactams associates with a composite genetic element, SCCmec cassette type V_T (5C2&5). We assigned the strain to sequence type ST338 (single-locus variant of ST59), clonal complex CC59, spa-type t437, and agr-type I. Genomic-based comparison was designated SO574/12 as an international Taiwan clone, which has been so far described mainly in the Asia-Pacific region. The ermB gene locates on the chromosome within the 14,690 bp mobile element structure, i.e., the MES_PM1-like structure, which also encodes aminoglycoside- and streptothricin-resistance genes. The MES_PM1-like structure is a composite transposon containing Tn551, flanked by direct repeats of IS1216V insertion sequences, which probably originates from Enterococcus. The ermB is preceded by the 273 bp regulatory region that contains the regulatory 84 bp ermBL ORF, encoding the 27 amino acid leader peptides. The latest research suggests that a new leader peptide, ermBL2, also exists in the ermB regulatory region. Therefore, the detailed function of ermBL2 requires further investigations.     

Synthesis,In Vitro,and Computational Studies of PTP1B Phosphatase Inhibitors Based on Oxovanadium(IV) and Dioxovanadium(V) Complexes

One of the main goals of recent bioinorganic chemistry studies has been to design and synthesize novel substances to treat human diseases. The promising compounds are metal-based and metal ion binding components such as vanadium-based compounds. The potential anticancer action of vanadium-based compounds is one of area of investigation in this field. In this study, we present five oxovanadium(IV) and dioxovanadium(V) complexes as potential PTP1B inhibitors with anticancer activity against the MCF-7 breast cancer cell line, the triple negative MDA-MB-231 breast cancer cell line, and the human keratinocyte HaCaT cell line. We observed that all tested compounds were effective inhibitors of PTP1B, which correlates with anticancer activity. [VO(dipic)(dmbipy)]·2 H₂O (Compound 4) and [VOO(dipic)](2-phepyH)·H₂O (Compound 5) possessed the greatest inhibitory effect, with IC₅₀ 185.4 ± 9.8 and 167.2 ± 8.0 nM, respectively. To obtain a better understanding of the relationship between the structure of the examined compounds and their activity, we performed a computer simulation of their binding inside the active site of PTP1B. We observed a stronger binding of complexes containing dipicolinic acid with PTP1B. Based on our simulations, we suggested that the studied complexes exert their activity by stabilizing the WPD-loop in an open position and limiting access to the P-loop.     

新型基于几何的3D非平稳V2V MIMO信道模型

针对具有多种通信场景和收发端快速移动的V2V通信系统,提出一种基于几何的3D V2V MIMO信道模型.该模型首次结合双球模型和半椭球体模型,分别使用双球和半椭球体模型表征动态和静止散射体;考虑到收发端的运动状态,引入时变的出发角、到达角以及路径长度用于研究V2 V信道的非平稳特性;对所提出的信道模型,推导了空间互相关、时间自相关和多普勒功率谱密度等统计特性函数,分析了不同场景和参数下的V2 V信道统计特性.结果表明,不同场景下V2 V信道各统计特性有较大差异,信道相关性与时间变化和散射体分布密切相关.仿真结果与理论值的高度拟合验证了模型的正确性,为V2 V通信链路的设计提供了理论依据,拓展了基于几何的V2 V信道建模领域的研究.     

基于Internet的虚拟企业资源计划V-ERP

虚拟企业（VE）是随着Internet而出现的。随着VE的出现,经历了从MRP、MRPⅡ到ERP的发展过程的计划技术必须跟着演进。文章在分析计划技术发展过程的基础上提出了基于Internet的虚拟企业资源计划（V－ERP）。V－ERP是由顾客需求驱动的,它可以集成不同计划方法来制订虚拟企业中所有合作伙伴的计划。文章分析了V－ERP的特点,并讨论了其构成。     

甚低带宽环境下的多媒体通信传输控制模型

介绍了基于H．324协议框架的可视电话基本原理．H．324协议是基于PSTN普通电话线路的多媒体可视通信协议．通过深入研究H．223数据复用协议和V．80同步传输协议，针对甚低带宽的PSTN线路特点，提出了一种有效的多媒体通信传输控制模型，改进了H．324可视电话的性能．     

Altera MAX V CPLD的工程设计应用及系统开发

本文阐述了Altera MAX V最小系统从硬件原理图到PCB设计、从软件QuartusII编写VHDL到Modelsim-Al-tera的软件仿真,以及最终的程序下载到开发板上的整个工程设计调试过程,为后续的Altera MAX V工程设计应用提供技术参考和支持.     

使用PT5.0模拟实现不同VLAN之间主机通讯

使用Packet Tracer V5.0（PT5.0）模拟软件可以在不影响教学质量的情况下,既节省了资金投入,又能满足正常的教学需要,提高了学习效率。介绍了PT5.0模拟软件的优点,三层交换机和路由器的区别,并以实例介绍了通过三层交换机和路由器来实现不同VLAN之间主机通讯的方法。     

基于5G波段的V2V高速无线信道建模

高速移动的车车(Vehicle-to-Vehicle,V2V)信道与传统蜂窝系统的无线信道相比,其不同在于V2V无线信道的快速时变特性,主要体现在多径分量的快速变化、存在较大多普勒频移以及每条多径都具有动态生/灭特性。提出了一种高速无线信道建模方法,采用马尔科夫链模拟信道的动态生/灭特性,基于Nakagami分布仿真莱斯、瑞利等信道单径衰落,用抽头延时线模型完成高速多径信道建模。与传统无线信道建模方式相比,具有快速建模、动态改变和易于产生的特点,为高速移动无线接收机算法的开发奠定了基础。仿真结果表明,城市车内天线(UIC)信道多径数目最多,衰落最严重。     

SDH专网的接入网网络管理系统设计

接入网是通信发展演变的主要方向之一,从网络管理的角度来看,接入网也是最复杂,最难管理的网络之一。由于光通信技术已经基本成熟,光纤接入网已成为当前接入网市场的主流。在本文SDH光纤接入网中,为了适应用户网络多种业务接入的需要,采用V5这一国际标准的数字接口作为接入网连接公共电信网的纽带。同时接入网中必须提供Q3接口,以实现电信管理网对接入网侧V5接口的管理。首先研究SDH光同步数字传输网络与专网通信。对基于本项目2.5G光纤接入平台的V5接入网,分析其V5接口结构、功能、相关协议以及V5接口系统的实现方案。在此基础上,探讨了电信管理网(TMN)的体系结构,相关协议与管理信息库。提出一种基于TMNQ3代理(Agent)的方案——管理者-代理-后台的设计方案,分析此方案的信息模型、通信协议栈及模块划分。接着,根据迈力特2.5G光纤综合业务传输网络的实际情况,在不更改此方案信息模型的基础上,用SNMP实现SDH接入网的管理并阐述其具体实现。在此之后,通过一致性测试和功能行测试,对该软件测试行进说明。     

仓鼠V79细胞对加速碳离子的辐照致死效应

分别测定了传能线密度（LET）为125．5、200、700keV／μm碳离子辐照仓鼠V79细胞的存活曲线,由存活曲线确定了上述3种碳离子辐照时V79细胞的失活截面依次为7．86±0．17、10．44±1．11、32．32±3．58μm2。以V79细胞对60Coγ射线的存活响应为参考值,给出了对应于上述3种碳离子照射周％、20％、50％、80％存活水平下的相对生物学效应（RBE）,结果表明125．5keV／μm碳离子的RBE值在各个存活水平下都为最大。提示：以存活为生物学终点的RBE最大值在LET值小于200keV／μm的碳离子辐照时出现。