基于家族优生学的电力系统故障诊断

引入基于家族优生学的进化（FEBE）方法进行电力系统的故障诊断。该方法将正交设计技术引入家庭的子代培养过程,以加强个体的行为改进,避免早熟,加速了进化后期的收敛速度。此方法用于保护和断路器不正常动作或多重故障等复杂情况下的电力系统故障诊断也有满意的效果。算例系统的研究结果证明了FEBE方法的应用提高了电力系统故障诊断的效率和准确率。     

Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.)

Members of the Mi14-3-3 gene family interact with target proteins that are widely involved in plant hormone signal transduction and physiology-related metabolism and play important roles in plant growth, development and stress responses. In this study, 14-3-3s family members are identified by the bioinformatic analysis of the mango (Mangifera indica L.) genome. The gene structures, chromosomal distributions, genetic evolution, and expression patterns of these genes and the physical and chemical properties and conserved motifs of their proteins are analysed systematically. The results identified 16 members of the 14-3-3 genes family in the mango genome. The members were not evenly distributed across the chromosomes, and the gene structure analysis showed that the gene sequence length and intron number varied greatly among the different members. Protein sequence analysis showed that the Mi14-3-3 proteins had similar physical and chemical properties and secondary and tertiary structures, and protein subcellular localization showed that the Mi14-3-3 family proteins were localized to the nucleus. The sequence analysis of the Mi14-3-3s showed that all Mi14-3-3 proteins contain a typical conserved PFAM00244 domain, and promoter sequence analysis showed that the Mi14-3-3 promoters contain multiple hormone-, stress-, and light-responsive cis-regulatory elements. Expression analysis showed that the 14-3-3 genes were expressed in all tissues of mango, but that their expression patterns were different. Drought, salt and low temperature stresses affected the expression levels of 14-3-3 genes, and different 14-3-3 genes had different responses to these stresses. This study provides a reference for further studies on the function and regulation of Mi14-3-3 family members.     

Supporting Product Family Design in a 3D CAD/PDM Integrated System

This paper addresses some key issues in supporting product family design in a 3 D CAD/ PDM integrated system and introduces the techniques developed or adopted in building the integrated system. The general architecture of a 3D CAD/PDM integrated system is organized as five tiers. Multi-agent technology is applied with a collaborative design environment. The rule-based product family architecture （ PFA ） and tabular layouts of article characteristics （TLAC） technique are applied to represent the knowledge for product families. They make the knowledge understandable, simplified and can be exchanged among heterogeneous information systems. A transparently concentric integrated mode is put forward to enable the heterogeneous processes to interact and communicate regardless of the language used to construct each process. With the mode, function calling and information exchanging among different processes are carried out via message translation/parse approach. Currently, the integrated system is implemented in the platform of Windows NT and 2000.     

基于多零件工序族工艺规划的机床产品构型综合设计

针对机床产品的定制问题,以基本工艺元为基础将多零件工序族进行组合,建立了工艺和机床结构的映射关系,建立了机床的运动功能模型并计算完成工序族所需要的运动范围,制定了机床的运动学图谱并根据约束条件进行选择,使机床的工艺能力在多零件工序组合的加工需求变化范围内,从而实现机床的定制柔性。工艺聚类和排序为实现以工艺能力为导向的机床结构设计提供了依据,保证了设计的定制性,最后以实例进行了说明。     

基于特征的参数化造型技术族研究与应用

对参数化设计技术、特征技术和基于参数化的特征造型技术做了系统地回顾,在深入地剖析上述技术的基础上,提出了综合运用各技术优点的基于特征的参数化造型技术族的概念及原理。最后给出了以Pro／E为平台,运用基于特征的参数化造型技术族原理于渐开线斜齿圆柱齿轮的实例。     

Combinatorial Measurement of CDKN1A/p21 and KIF20A Expression for Discrimination of DNA Damage-Induced Clastogenicity

In vitro mammalian cytogenetic tests detect chromosomal aberrations and are used for testing the genotoxicity of compounds. This study aimed to identify a supportive genomic biomarker could minimize the risk of misjudgments and aid appropriate decision making in genotoxicity testing. Human lymphoblastoid TK6 cells were treated with each of six DNA damage-inducing genotoxins (clastogens) or two genotoxins that do not cause DNA damage. Cells were exposed to each compound for 4 h, and gene expression was comprehensively examined using Affymetrix U133A microarrays. Toxicogenomic analysis revealed characteristic alterations in the expression of genes included in cyclin-dependent kinase inhibitor 1A (CDKN1A/p21)-centered network. The majority of genes included in this network were upregulated on treatment with DNA damage-inducing clastogens. The network, however, also included kinesin family member 20A (KIF20A) downregulated by treatment with all the DNA damage-inducing clastogens. Downregulation of KIF20A expression was successfully confirmed using additional DNA damage-inducing clastogens. Our analysis also demonstrated that nucleic acid constituents falsely downregulated the expression of KIF20A, possibly via p16 activation, independently of the CDKN1A signaling pathway. Our results indicate the potential of KIF20A as a supportive biomarker for clastogenicity judgment and possible mechanisms involved in KIF20A downregulation in DNA damage and non-DNA damage signaling networks.     

A Modification of schwarz's sequential likelihood ratio tests in multivariate sequential analysis

By developing and applying certain results on boundary crossing probabilities of generalized likelihood ratio statistics in multiparameter exponential families, we show that Lai's (1988a) modification of Schwarz's (1962) sequential likelihood ratio tests of one-sided hypotheses in the one-parameter case can be extended to general hypotheses in the multiparameter case. Such tests are shown to be asymptotically optimal, from both frequentist and Bayesian points of view, and numerical results are also given to demonstrate their performance.     

填料生物转盘对农村家庭生活污水的处理

根据目前我国农村生活污水处理的现状以及农村家庭污水水量、水质的特点,提出了一种家庭生活污水处理装置-填料生物转盘。该装置采用生物膜法工艺,结合生物转盘与生物滤池工艺的特点,运用设备一体化的设计理念,设计了一种运行管理简单、结构紧凑的一体化装置。通过实际运行考察了装置用于农村家庭生活污水处理的挂膜启动情况,以及污染物的去除效果。结果表明:填料生物转盘生物膜的驯化培养需要20天的时间,稳定运行期间对COD、NH4+-N、TN、TP的平均去除率分别稳定在75%、85%、65%和75%以上,出水COD、NH4+-N、TN优于城镇污水处理厂污染物排放标准(GB 18918-2002)一级A标准,出水TP达到一级B标准。装置运行管理操作简单,不需要专业技术人员,适合农村家庭污水的处理。     

Orientated Immobilization of FAD-Dependent Glucose Dehydrogenase on Electrode by Carbohydrate-Binding Module Fusion for Efficient Glucose Assay

The discovery or engineering of fungus-derived FAD-dependent glucose 1-dehydrogenase (FAD-GDH) is especially important in the fabrication and performance of glucose biosensors. In this study, a novel FAD-GDH gene, phylogenetically distantly with other FAD-GDHs from Aspergillus species, was identified. Additionally, the wild-type GDH enzyme, and its fusion enzyme (GDH-NL-CBM2) with a carbohydrate binding module family 2 (CBM2) tag attached by a natural linker (NL), were successfully heterogeneously expressed. In addition, while the GDH was randomly immobilized on the electrode by conventional methods, the GDH-NL-CBM2 was orientationally immobilized on the nanocellulose-modified electrode by the CBM2 affinity adsorption tag through a simple one-step approach. A comparison of the performance of the two electrodes demonstrated that both electrodes responded linearly to glucose in the range of 0.12 to 40.7 mM with a coefficient of determination R² > 0.999, but the sensitivity of immobilized GDH-NL-CBM2 (2.1362 × 10⁻² A/(M*cm²)) was about 1-fold higher than that of GDH (1.2067 × 10⁻² A/(M*cm²)). Moreover, a lower detection limit (51 µM), better reproducibility (<5%) and stability, and shorter response time (≈18 s) and activation time were observed for the GDH-NL-CBM2-modified electrode. This facile and easy immobilization approach used in the preparation of a GDH biosensor may open up new avenues in the development of high-performance amperometric biosensors.     

Testing Parameter Change in General Integer‐Valued Time Series

We consider the structural change in a class of discrete valued time series, which the conditional distribution belongs to the one‐parameter exponential family. We propose a change point test based on the maximum likelihood estimator of the model's parameter. Under the null hypothesis (of no change), the test statistic converges to a well‐known distribution, allowing the calculation of the critical value of the test. The test statistic diverges to infinity under the alternative, meaning that the test has asymptotic power one. Some simulation results and real data applications are reported to show the effectiveness of the proposed procedure.