数字图书馆网络安全防御系统的构建

数字图书馆为人们的日常工作学习提高了很多专业有效的数据,它拥有巨大的数据容量,但一旦受到攻击,将造成巨大损失,给人们带来诸多不便。本文针对如何有效的构建数字图书馆网络安全防御系统这一问题进行了相关分析探讨。首先列出了几大危害网络安全的因素,再根据这些因素从加强网络技术防护、规范人文管理两方面展开研究。     

A Novel Artificially Humanized Anti-Cripto-1 Antibody Suppressing Cancer Cell Growth

Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto-1 antibody was produced and purified. The purified antibody showed affinity to recombinant Cripto-1 at 1.1 pmol and immunoreactivity to cancer tissues and cell lines. The antibody was available to detect the immunoreactivity in tissue microarrays of malignant tumors as well as in Cripto-1 overexpressing cells. Simultaneously, the antibody exhibited the potential to suppress the growth of human colon cancer derived GEO cells overexpressing Cripto-1 with IC₅₀ at approximately 110 nM. The artificially humanized antibody is proposed to be a good candidate to target cancer cells overexpressing Cripto-1.     

图书馆促进社会包容性发展的思考

促进社会包容性发展是图书馆从诞生伊始就坚守的价值观,通过保障信息自由与信息公平、促进多元文化理解、提高公民媒介信息素养等途径,图书馆致力于支持和促进社会包容性发展。图书馆需要进一步强化服务意识、加强跨组织协同、切实发挥信息素养培养及社区教育的服务职能、促进社区参与,为社会包容性发展提供更大支持。     

基于Windows的液压实时测试浅析

本文通过分析WINDOWS实时处理的局限性，提出了解决问题的几种机制；同时以液压元件的实时测试中断程序的实现为例说明了机制的应用，研究表明，这种机制较传统方法实用、有效。     

Antibody Libraries as Tools to Discover Functional Antibodies and Receptor Pleiotropism

Most antibodies currently in use have been selected based on their binding affinity. However, nowadays, antibodies that can not only bind but can also alter the function of cell surface signaling components are increasingly sought after as therapeutic drugs. Therefore, the identification of such functional antibodies from a large antibody library is the subject of intensive research. New methods applied to combinatorial antibody libraries now allow the isolation of functional antibodies in the cellular environment. These selected agonist antibodies have provided new insights into important issues of signal transduction. Notably, when certain antibodies bind to a given receptor, the cell fate induced by them may be the same or different from that induced by natural agonists. In addition, combined with phenotypic screening, this platform allows us to discover unexpected experimental results and explore various phenomena in cell biology, such as those associated with stem cells and cancer cells.     

Chemical Modification of Phage-Displayed Helix-Loop-Helix Peptides to Construct Kinase-Focused Libraries

Conformationally constrained peptides hold promise as molecular tools in chemical biology and as a new modality in drug discovery. The construction and screening of a target-focused library could be a promising approach for the generation of de novo ligands or inhibitors against target proteins. Here, we have prepared a protein kinase-focused library by chemically modifying helix-loop-helix (HLH) peptides displayed on phage and subsequently tethered to adenosine. The library was screened against aurora kinase A (AurA). The selected HLH peptide Bip - 3 retained the α-helical structure and bound to AurA with a K_D value of 13.7 μM. Bip - 3 and the adenosine-tethered peptide Bip - 3 - Adc provided IC₅₀ values of 103 μM and 7.7 μM, respectively, suggesting that Bip - 3 - Adc bivalently inhibited AurA. In addition, the selectivity of Bip - 3 - Adc to several protein kinases was tested, and was highest against AurA. These results demonstrate that chemical modification can enable the construction of a kinase-focused library of phage-displayed HLH peptides.     

基于计算机网络的移动图书馆系统构建研究

随着移动互联网技术快速发展,图书馆事业迎来了前所未有的发展机遇。移动互联网涵盖面广、便捷性高、用户可以随时随地通过网络访问图书馆资源。普及移动图书馆服务,是我国目前图书馆事业的主要发展方向之一。本文简单介绍了互联网环境,从技术角度和系统建设两个方面阐述了这一主题。     

基于Host Link FINS协议的PLC远程通信

以欧姆龙公司CP1H型号的PLC为应用背景,设计了基于Host Link FINS协议的PLC设备远程监控系统.该文提出了基于串口的远程通信方案,并且详细分析了Host Link FINS协议的结构和指令格式,用MFC实现了监控软件的设计,完成监控软件与欧姆龙PLC的数据通信功能,实现监控软件对PLC的远程监控.经过现场的测试和运行,该监控系统稳定可靠,性能较高.     

基于IOS平台的小型仓储管理系统设计

随着数字化、信息化技术在物流领域应用的飞速发展,各种各样的仓储管理系统日益受到关注。本文介绍的基于IOS平台的小型仓储管理系统是利用IOS设备对货物信息进行采集,通过SQLite数据库技术、ZXing库等软硬件方式构建的业务系统,可以较好地适应中小规模用户数据交互的需求。同时,系统具备造价低廉、操作便捷等优点。     

试谈云储存在数字化图书馆中的实践应用

在数字化图书馆的发展建设过程中,云储存网络服务技术在当前受到越来越多工程技术人员的关注与支持。云储存服务平台通过将多种网络技术与服务有机结合,对数字图书馆中的知识资源进行整合,从而实现数字图书馆的创新发展。通过论述在数字化图书馆中云储存的实践应用状况,分析提高图书馆服务水平的技术方法,以切实推动数字化图书馆的发展进程。