Experiments conducted in the laboratory tested the hypotheses that aggregated oviposition by onion maggot flies,Delia antiqua (Meigen), is caused by stimuli associated with ovipositing females, newly laid eggs, or both. Using a paired oviposition station bioassay that eliminated visual stimuli associated with the treatment under study, 67% of the eggs laid by caged females were in response to the odor of females already ovipositing on an onion slice, as opposed to 33% of the eggs laid in response to an onion slice alone. When newly laid eggs were transferred to onion slices and held for either 24 or 48 hr before being bioassayed against similarly aged untreated onions, 74% and 97% of the eggs were laid at the egg-treated onion stations, respectively. Similar results were achieved when an aqueous wash of newly laid eggs was applied to the onion slice. When the egg wash was processed through a bacterial filter or when eggs were present but not in contact with onions, all response was eliminated. These results implicate microorganisms transmitted on the egg surface in creating an attraction for ovipositing females. Heptane extracts of ovipositor tips from mated, ovipositing females induced 72% of the test females to oviposit near points at which extracts were applied to the oviposition station floor. A behavioral sequence for an optimal host-selection strategy is hypothesized, whereby host-seeking female onion flies respond to host-derived alkyl sulfides at long range and metabolic by-products of microbially infested hosts and visual cues at short range (ca. l m), with final selection of oviposition sites potentially reinforced by contact with an aggregation pheromone released or left on the substrate by ovipositing females. 相似文献
The European shore crab Carcinus maenas is considered to rely on a female pheromone when mating. Evidence, however, is scarce on how the urine pheromone in itself affects males. We investigated male primer and releaser responses to female pheromones with methods that minimized effects from females, delivering female urine either as a pump-generated plume or deposited on a polyurethane sponge. We delivered the pheromone at different concentrations in far, near, and close/contact range to get a picture of how distance affects behavioral response. Our results show that substances in premolt female urine (PMU) function as primer and potent short-range releaser pheromones. Based on the olfactometer and sponge tests, we conclude that PMU stimulus in itself is sufficient to elicit increased search and mating-specific behaviors such as posing, posing search, cradle carrying, and stroking. Pheromone concentrations do not seem to be important for attenuating search and posing as long as the level is above a certain threshold concentration. Instead, pheromone levels seem to play a role in male acceptance of females, recruiting more males to respond, and generating better responses with increasing concentration. 相似文献
Mature female apple maggot flies,Rhagoletis pomonella (Walsh), were released individually onto a single potted, fruitless hawthorne tree in the center of an open field. The tree was surrounded by four 1-m2 plywood host tree models painted green or white, with or without synthetic host fruit odor (butyl hexanoate), and placed at one of several distances from the release tree. Each fly was permitted to forage freely on the release tree for up to 1 hr, or until it left the tree. Flies left the tree significantly sooner when green models with host fruit were present at 0.5, 1.5, or 2.5 m distance from the release tree than when these models were placed at a greater distance (4.5 m) from the release tree or when no models were present. Flies responded detectably to 1-m2 models without odor up to a maximum distance of 1.5 m. These results suggest that female apple maggot flies did not detect green 1-m2 models with odor 4.5 m away or models without odor 2.5 m or more away. Flies responded to white models with and without odor to a much lesser extent, both in terms of response distance and flight to and alightment upon models. Increasing model size to 2 m2 increased the distance to 2.5 m at which flies responded to green models without odor. Decreasing model size to 0.5 m2 reduced fly responsiveness to green or white models. The presence of host fruit odor alone, without the visual stimulus of a green model, did not influence residence time on the release tree. 相似文献
The elderly person's perception of foods and food flavor is affected by age‐associated changes in the chemical senses (taste, smell, and trigeminal sensation). Both classic and modern psychophysical techniques have been applied to achieve some understanding of these changes.
Taste threshold sensitivity declines with age; however, the magnitude of the decline and the degree to which taste qualities are differentially affected remains to be understood. Suprathreshold taste intensity perception is affected by age, but there is a differential effect of taste quality.
Experiments with blended foods have indicated that both olfactory and taste deficits contribute to older people's difficulty with food identification. Experiments assessing threshold sensitivity, suprathreshold intensity, and suprathreshold identification have all demonstrated significant impairment in olfaction in old age. In fact, these effects are far greater than in the taste system.
Patients with Alzheimer's disease show even greater olfactory deficits than normal elderly and the effect is related to the degree of dementia. We have ruled out nasal disease in these patients as the primary causes of olfactory insensitivity, because significant impairment remains when the influence of nasal airflow and nasal cytology has been removed statistically. Both normal elderly and patients with Alzheimer's disease show impairment in odor memory. Sensitivity to, familiarity with, and identifiability of the odors all play a role in odor memory performance.
Flavor preference also changes over the lifespan. Older subjects, for instance, rate high concentration of sugar and salt as pleasanter than young subjects do. Multiple influences contribute to this phenomenon. Elderly persons and those of lower nutritional status have shown preferences for higher concentrations of casein hydrolysate than young persons and those of higher nutritional status, suggesting that dietary preference can be related to chemosensory cues.
There are significant age‐associated changes in chemosensory perception that have the potential to interact with dietary selection and nutrition in the elderly. A better understanding of these phenomena may promote health and well‐being in the elderly. 相似文献
Male neotropical orchid bees (Euglossini) collect odoriferous substances from orchids and other sources and store them in
tibial pouches, accumulating complex and species-specific bouquets. These fragrances are later exposed at display sites, presumably
to attract females or conspecific males or both. We hypothesized that the necessity to detect and recognize specific fragrance
bouquets has led to peripheral chemosensory specializations in different species of orchid bees. To test this, excised male
antennae of four species of Euglossa were stimulated with complete tibial extracts of the same four species in a crosswise experiment. In the majority of the
tested extracts, the amplitude of the electroantennogram (EAG) response was significantly different between species and always
maximal in males of the extracted species. This effect did not appear to result from a given species' increased sensitivity
toward certain attractive components: gas chromatography with electroantennographic detection (GC-EAD) of one extract of Euglossa tridentata evoked similar and generalized response patterns in all four species, encompassing a total of 34 peaks that elicited antennal
responses. Therefore, the species effect in EAG responses to complete extracts likely resulted from species-specific interactions
of compounds at the receptor level. Antennal specialization to conspecific bouquets adds additional strength to the argument
that specificity is an important evolutionary aspect of euglossine tibial fragrances.
Electronic supplementary material Supplementary material to this paper is available in electronic form at 相似文献