Critical-point drying versus freeze drying for scanning electron microscopy: a quantitative and qualitative study on isolated hepatocytes

Critical-point drying and freeze drying were compared both quantitatively and qualitatively as preparative procedures for scanning electron microscopy. Isolated hepatocytes were used as model cells. Nomarski differential interference contrast microscopy was used for light microscopic measurements of the hepatocytes in the unfixed, the glutaraldehyde fixed, the glutaraldehyde + OsO₄ fixed, the critical-point dried and the freeze dried states. Critical-point dried hepatocytes were found to shrink to 38% of glutaraldehyde + OsO₄ fixed volume, whereas optimal freeze dried hepatocytes (frozen in water saturated with chloroform and freeze dried at 183 K for 84 h) were found to shrink to 51% of glutaraldehyde + OsO₄ fixed volume. Transmission and scanning electron micrographs of the critical-point dried cells showed well-preserved ultrastructure and surface structure. Micrographs of the freeze dried cells showed ultrastructure destroyed by internal ice crystals and surface structure destroyed by external ice crystals. Double-fixed isolated hepatocytes were shown to swell during storage in buffer and to shrink during storage after critical-point drying. For low magnification scanning electron microscopy (up to about 3000 times) both critical-point drying and freeze drying can be used. However, for high magnification scanning electron microscopy, critical-point drying is superior to freeze drying.     

涡轮钻具在高陡构造的应用及展望

介绍了涡轮钻具在秋参1井的应用情况,详细叙述了涡轮钻具在山前高陡构造快速钻进及防斜打直方面的优点,与常规钻井相比,机械钻速明显提高,钻井周期明显缩短,钻井成本明显下降,经济效益显著。     

地地战术导弹飞行可靠性指标评定方法

在小子样情况下 ,利用系统和分系统试验数据对导弹的飞行可靠性指标进行评定 ,从数学上说是系统可靠性综合问题 .运用MML SR方法对新型号地地战术导弹的飞行可靠性指标进行评定 ,避免了MML方法在遇到无失败单元时不适用的缺陷 ,在利用地面试验信息方面有其明显优势 ;运用Bayes方法对改进型导弹的飞行可靠性指标进行评定 ,将不同试验阶段的多源试验信息作为验前信息 ,扩大了信息量 .最后 ,对两种方法在工程应用上遇到的问题进行了讨论 ,并给出了解决方法 .     

Trypsin Inhibitor Activity In Vitro Digestibility and Sensory Quality of Meat-Like Yuba Products as Affected by Processing

ABSTRACT: :
Soybeans ( Glycine max ) were soaked and ground to obtain soymilk. The soymilk was cooked in an open tank and held at 85 to 90 deg;C. Yuba films were picked up in 20 min intervals and dried for 20 min. Yuba films were soaked in chicken-flavor solutions (25% and 35%), and baking soda (BS) solutions (0%, 1%, 2%, and 3% BS), and cooked at 100 °C for 30 min, 60 min, and 90 min. TIA decreased (p < 0.05) with the increase of heating time and BS concentration. In vitro protein digestibility (IVPD) decreased with heating time and BS concentration (p < 0.05). Sensory characteristics were affected by flavor concentration. By using 0% BS, 25% of the chicken flavor concentration, and a short heating time method, meat-like products with low TIA, high IVPD, and good sensory characteristics were obtained.     

中厚板表面质量分析

在对济钢中厚板各种表面缺陷产生原因和几率进行综合分析的基础上 ,将缺陷分为 3类 :A类(折叠、麻点、划伤等 )、B类 (夹杂、结疤、分层 )、C类 (裂纹 ) ,重点对裂纹缺陷进行探讨 ,并提出了相应的改进措施。     

定向棱镜腔平顶高斯激光束特性的研究

在XeCl准分子激光器中,采用定向棱镜作为端反射镜与平面输出镜构成激光器谐振腔,获得了近场能量分布均匀、远场能量分布高度集中的相干平顶高斯激光束输出,并具有腔镜高抗失调能力,改善了激光束质量。     

改进尿素包装工艺 不断提高产品质量

通过对尿素包装工艺的不断改进，提高了尿素粒度、减少粉尘含量，效果明显。     

电子产品可靠性设计与成本控制

介绍了可靠性设计过程涉及的质量控制成本的解决方法，通过对最有可能影响可靠性的电路设计、元器件选用等方面的分析，介绍了在降低可靠性设计成本的基础下更好地解决可靠性问题的一些方法。     

分布式光纤喇曼放大技术及其在WDM系统中的应用

介绍了分布式光纤喇曼放大技术的原理、特点和应用 ,并从受此影响的 WDM传输系统的信噪比、噪声指数和品质因数三个方面讨论了光纤喇曼放大技术在提升光纤通信系统性能方面的重要作用。     

振动力场作用下聚合物挤出制品结构与性能的质量控制与优化

利用实验数据，分析与研究振动场振动参数对聚合物挤出制品质量的影响，对聚合物挤出制品的熔体流动速率，微晶结构，拉伸强度等主要性能质量指标，建立以振动频率为主要控制变量的神经网络模型，并引入信息分配模型，探讨了一个网络输入节点下神经网络学习样本的特征提取与优化。实验结果表明，经过信息预处理的学习样本，可以使网络有更好的收敛效果。