Overexpression of microRNAs miR-25-3p,miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction,Validation of Results and Study of the Biochemical Pathways Involved

In a prospective study, 48 fetuses were evaluated with Doppler ultrasound after 34 weeks and classified, according to the cerebroplacental ratio (CPR) and estimated fetal weight (EFW), into fetuses with normal growth and fetuses with late-onset fetal growth restriction (LO-FGR). Overexpression of miRNAs from neonatal cord blood belonging to LO-FGR fetuses, was validated by real-time PCR. In addition, functional characterization of overexpressed miRNAs was performed by analyzing overrepresented pathways, gene ontologies, and prioritization of synergistically working miRNAs. Three miRNAs: miR-25-3p, miR-185-5p and miR-132-3p, were significantly overexpressed in cord blood of LO-FGR fetuses. Pathway and gene ontology analysis revealed over-representation of certain molecular pathways associated with cardiac development and neuron death. In addition, prioritization of synergistically working miRNAs highlighted the importance of miR-185-5p and miR-25-3p in cholesterol efflux and starvation responses associated with LO-FGR phenotypes. Evaluation of miR-25-3p; miR-132-3p and miR-185-5p might serve as molecular biomarkers for the diagnosis and management of LO-FGR; improving the understanding of its influence on adult disease.     

钨含量及固溶温度对022Cr25Ni7Mo3.5WCuN钢耐腐蚀性能的影响

研究了钨含量及固溶温度对超级双相不锈钢022Cr25Ni7Mo3.5WCuN耐点腐蚀性能的影响。通过化学浸泡失重法和电化学极化曲线法,测试了超级双相不锈钢022Cr25Ni7Mo3.5WCuN耐点腐蚀性能,并运用Thermo Calc热力学计算辅助分析。结果表明,固溶温度对超级双相不锈钢022Cr25Ni7Mo3.5WCuN耐点腐蚀性能影响效果显著,在1 100 ℃时,022Cr25Ni7Mo3.5WCuN 的耐腐蚀性能达到最佳;在理想的固溶条件下,钨元素有助于钝化膜的形成,钨含量的增加使得022Cr25Ni7Mo3.5WCuN的耐腐蚀性能增强,在钨质量分数为1.5%时,022Cr25Ni7Mo3.5WCuN获得最佳耐腐蚀性能,若钨含量继续增加,打破了α和γ两相的平衡,则耐蚀性能降低。     

Identification and In Silico Characterization of a Novel COLGALT2 Gene Variant in a Child with Mucosal Rectal Prolapse

Rectal prolapse is influenced by many factors including connective tissue dysfunction. Currently, there is no data about genetic contribution in the etiology of this disorder. In this study, we performed trio whole-exome sequencing in an 11-year-old girl with mucosal rectal prolapse and her parents and sibling. Genetic testing revealed a novel heterozygous missense variant c.1406G>T; p.G469V in exon 11 of the COLGALT2 gene encoding the GLT25 D2 enzyme. Sanger sequencing confirmed this variant only in the patient while the mother, father and sister showed a wild-type sequence. The pathogenicity of the novel variant was predicted using 10 different in silico tools that classified it as pathogenic. Further, in silico prediction, according to Phyre2, Project HOPE, I-Mutant3.0 and MutPred2 showed that the missense variant can decrease protein stability and cause alterations in the physical properties of amino acids resulting in structural and functional changes of the GLT25D2 protein. In conclusion, the present study identifies a previously unknown missense mutation in the COLGALT2 gene that encodes the enzyme involved in collagen glycosylation. The clinical features observed in the patient and the results of in silico analysis suggest that the new genetic variant can be pathogenic.     

Finite sample properties of Moran's I test for spatial autocorrelation in tobit models

In this note, we investigate the finite‐sample properties of Moran's I test statistic for spatial autocorrelation in tobit models suggested by Kelejian and Prucha. We fill a void in the theoretical literature by investigating the finite sample properties of this test statistic in a series of Monte Carlo simulations, using data sets ranging from 49 to 15,625 observations. We find that the test is unbiased, has considerable power and approximates the asymptotic normal distribution even for medium‐sized sample sizes, empirically confirming the theoretical results of Kelejian and Prucha. However, some caution is needed, since the statistic turns out to be sensitive to misspecification in the form of heteroscedasticity. In such instances the test over‐rejects the null hypothesis, mistaking heteroscedasticity for spatial autocorrelation.     

L.paracasei HD1-7与S.albulus1-25原生质体融合选育产天然防腐剂的优良菌株

为获得更具优良应用特性的微生物源天然防腐剂,采用原生质体融合技术,以Lactobacillus paracasei HD1-7和Streptomyces albulus1-25为亲株选育优良菌株,研究了L.paracasei HD1-7和S.albulus1-25原生质体制备与再生条件,通过表型、抑菌谱、抗药性和总DNA含量比较等手段筛选鉴定融合子,最终选育出3株以L.paracasei HD1-7为受体、获得来自S.albulus1-25的抑酵母菌基因的融合子。     

微观相场法研究沉淀早期两相竞争对筏化的影响

基于微观相场动力学模型,研究沉淀早期不同成分Ni-Al-V合金时效时有序相的生长和组织生长方向的变化。结果表明:沉淀早期有序相间的竞争生长会很大程度地影响合金的形貌及生长方向。在一定的温度、应力场作用下,Al含量较低时,DO22有序相先析出,DO22相颗粒数量较多、尺寸较小,有序相的筏化不明显,合金整体上无显著的筏化现象;Al含量稍高时,L12相先析出,DO22相颗粒数量较少、尺寸较大,组织在[010]方向上发生一定程度的择优生长;Al含量较高时,L12相先析出,DO22相颗粒数量继续减少、尺寸继续增大,组织在[100]方向上显示出较强的筏化趋势;较低温度时效的合金L12有序相析出提前,组织的筏化趋势较弱。     

Ti-22Al-25Nb合金大规格棒材组织与力学性能研究

本文研究了通过逐步降温锻造工艺制备的Ti₂AlNb基合金大规格(Ф300 mm)棒材的组织均匀性,分析了热处理前后棒材不同部位显微组织的变化,并进行了力学性能测试。结果表明：采用逐步降温锻造工艺制备的Ф300 mm棒材靠近外表面区域变形比较充分,显微组织均匀性较好,而靠近心部区域的显微组织均匀性较差,小区域存在未完全破碎的晶界,这种差别会遗传到后续热处理态的显微组织中。通过对比棒材不同部位经热处理后的力学性能,发现不同部位性能差异不大,而心部区域的强度低于表面区域,而塑性则呈现相反的变化趋势,这可能与不同区域由于变形程度不均匀导致了α相尺寸及含量的差异所造成。     

SiC和Al2O3颗粒对Ti-25Nb-3Mo-3Zr-2Sn合金微磨损行为的影响

本文研究了SiC和Al₂O₃颗粒对生物医用植入材料Ti-25Nb-3Mo-3Zr-2Sn合金的微磨损行为的影响。在文中研究了合金的比磨损率、摩擦系数、磨损机制以及微磨损与腐蚀之间的协同作用。研究结果显示,合金的比磨损率随磨粒尺寸的增加而增加。由于SiC磨粒的硬度和切削性均优于Al₂O₃磨粒,由此在同尺寸磨粒下SiC磨粒所造成的材料的损失比Al₂O₃磨粒造成的要大。摩擦系数的结果显示,在同一种磨粒作用下,Hank’s溶液中所获得的摩擦系数的平均值大于蒸馏水中所获得的摩擦系数平均值,且由于Hank’s溶液的腐蚀性所致在Hank’s溶液中获得的摩擦系数的稳定性要比在蒸馏水中的稳定性差;在同尺寸磨粒下,Al₂O₃磨粒所造成的摩擦系数要大于SiC磨粒所造成的。随磨粒尺寸的减小,磨损机制由三体磨损转变为混合磨损之后再转变为二体磨损。从磨损机制图中可以看出腐蚀对材料损失的贡献是不容小视的,磨损机制为以磨损为主的磨损腐蚀。     

喷丸对25CrNi2MoV钢滚动接触疲劳性能的影响

目的提高25CrNi2MoV钢的滚动接触疲劳性能。方法对25CrNi2MoV钢进行表面喷丸处理,并采用3D形貌仪、光学显微镜、显微硬度仪、X射线应力分析仪与滚动接触疲劳试验机等仪器,对试样表面形貌、表面显微组织、显微硬度、表面残余压应力与滚动接触疲劳性能等进行测试分析。结果与未处理试样相比,经喷丸处理后,试样表面形貌由磨削加工槽型向酒窝状的弹坑转变,表面粗糙度增大,表面显微硬度由503HV0.2增大到577HV0.2,增加了14.7%,表面残余压应力由-90.0 MPa增大到-758.0 MPa。当喷丸强度为0.445 mmA时,试样具有最好的滚动接触疲劳寿命,其额定寿命(L10)、中值寿命(L50)、特征寿命(L63.2)分别为4.973×10^6次、6.578×10^6次和6.945×10^6次,分别是未处理试样对应寿命的11.1倍、7.3倍和7.0倍,试样滚动接触疲劳失效形式主要为疲劳剥落。当喷丸强度为0.596 mmA时,试样表面出现微裂纹,导致滚动接触疲劳寿命降低,此时试样疲劳失效形式主要为点蚀与疲劳剥落。未处理试样疲劳失效形式主要为分层。结论喷丸处理能细化试样表层晶粒组织,增大试样表面粗糙度、表面硬度与表面残余压应力。合适强度的喷丸处理可以抑制试样表面与次表面裂纹的萌生与扩展,显著提高滚动接触疲劳性能。