基于变论域模糊PI的永磁同步电机无传感控制

受到带通滤波器的影响,永磁同步电机(PMSM)脉振注入法中转子位置估算精度较低。为此,提出了一种基于变论域模糊PI的PMSM无传感控制法。利用变论域模糊的方法对转速环上固定的PI参数进行动态调节,达到减少系统延时、提高转子位置估算精度的目的。仿真的结果表明,变论域模糊PI能够用于转子位置估算,并且比传统PI无传感控制法减少了0.331°平均误差。     

GIL机械故障诊断与预警技术研究

为了有效诊断气体绝缘金属封闭输电线路(GIL)的机械故障,搭建了110 kV GIL试验平台并设计了3种典型机械故障,通过互补集合经验模态分解(CEEMD)模糊熵值与鲸鱼优化极限学习机(WOA-ELM)模型联合方法对GIL机械故障模式进行识别与诊断。首先,利用CEEMD方法对振动信号进行分解,引入正负白噪声组对信号进行处理,得到含有故障信息的模态分量(IMF)。其次,利用模糊熵计算模态分量特征值,得到能表征故障特征的模糊熵值。最后,结合WOA-ELM模型对特征向量集进行模式识别,根据聚类结果与自适应阈值对GIL设备机械故障进行诊断和预警。结果表明,利用CEEMD与模糊熵对GIL振动信号特征进行分析,可以有效避免模态混叠和冗余噪声分量的干扰,得到能够表征故障特征的特征值;利用WOA-ELM模型可以有效实现GIL设备机械故障诊断与预警。     

双向准Z源逆变器驱动永磁同步电机的快速有限集模型预测控制

双向准Z源逆变器驱动永磁同步电机(PMSM)系统在兼具Z源逆变器和PMSM优点的同时,还可实现能量的双向流动。针对双向准Z源逆变器驱动PMSM系统的特点,提出一种快速矢量选择有限集模型预测电流控制(FCS\|MPCC)策略。由双向准Z源网络直流链电压闭环与PMSM电磁功率前馈生成电感电流参考值,由电机转速闭环生成电机电流参考值。通过预测直通(ST)及非直通(NST)状态下的电感电流值并引入电感电流子代价函数以确定是否选择ST状态,进而实现对直流链电压的控制。在NST状态下,结合空间电压矢量脉宽调制策略,仅使目标电压矢量所在扇区的4个矢量参与PMSM定子电流预测和代价函数计算,以选择最优的开关状态,在实现对PMSM转速控制的同时减小在线计算量。仿真结果表明,所提控制策略可实现对双向准Z源逆变器的升压及PMSM牵引或制动工况下的转速控制,系统具有良好的稳态及动态性能。     

Improving antibacterial,biocompatible, and reusable properties of polyvinyl chloride via the addition of aluminum alkoxides

Global bacterial infections associated with conventional polyvinyl chloride (PVC) medical devices place a heavy burden on healthcare systems and thus it will be desirable if medical devices are made from antimicrobial PVC. There are numerous studies focusing on polymer surface modifications to either leach antimicrobial agents or kill pathogenic microbes upon direct contact. In this work, mannitol fumarate ester-based aluminum metal alkoxide (MFE-Al) additive was developed to confer simultaneously improved antibacterial property and enhanced high temperature sterilization resistance of the resultant PVC. Data obtained confirm that the MFE-Al stabilized PVC sheets significantly inhibit 98% bacterial growth. They also show biocompatibility with cultured H9C2 cardiomyocytes and hemocompatibility in vitro. Dry heat sterilization is generally not suitable for PVC medical wares due to their poor thermal compatibility. Surprisingly, our antimicrobial-biocompatible PVC can maintain stability at 180°C for 90 min. Such a high thermal stability indicates the MFE-Al stabilized PVC can endure 90 cycles of dry-heat sterilization without significant damage. This study may provide a solution to reduce PVC medical waste for a maximum benefit without compromising human health or the environment.     

mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors

Numerous molecular factors disrupt the correctness of the cell cycle process leading to the development of cancer due to increased cell proliferation. Among known causative factors of such process is abnormal gene expression. Nowadays in the light of current knowledge such alterations are frequently considered in the context of mRNA–miRNA correlation. One of the molecular factors with potential value in tumorigenesis is the feedback loop between MYC and E2F genes in which miR-17-5p and miR-20a from the miR-17-92 cluster are involved. The current literature shows that overexpression of the members of the OncomiR-1 are involved in the development of many solid tumors. In the present work, we investigated the expression of components of the MYC/E2F/miR-17-92 network and their closely related elements including members of MYC and E2F families and miRNAs from two paralogs of miR-17-92: miR-106b-25 and miR-106a-363, in the most common brain tumors of childhood, pilocytic astrocytoma (PA), WHO grade 1; ependymoma (EP), WHO grade 2; and medulloblastoma (MB), WHO grade 4. We showed that the highest gene expression was observed in the MYC family for MYCN and in the E2F family for E2F2. Positive correlation was observed between the gene expression and tumor grade and type, with the highest expression being noted for medulloblastomas, followed by ependymomas, and the lowest for pilocytic astrocytomas. Most members of miR-17-92, miR-106a-363 and miR-106b-25 clusters were upregulated and the highest expression was noted for miR-18a and miR-18b. The rest of the miRNAs, including miR-19a, miR-92a, miR-106a, miR-93, or miR-25 also showed high values. miR-17-5p, miR-20a obtained a high level of expression in medulloblastomas and ependymomas, while close to the control in the pilocytic astrocytoma samples. miRNA expression also depended on tumor grade and histology.     

Genetic Insight into the Domain Structure and Functions of Dicer-Type Ribonucleases

Ribonuclease Dicer belongs to the family of RNase III endoribonucleases, the enzymes that specifically hydrolyze phosphodiester bonds found in double-stranded regions of RNAs. Dicer enzymes are mostly known for their essential role in the biogenesis of small regulatory RNAs. A typical Dicer-type RNase consists of a helicase domain, a domain of unknown function (DUF283), a PAZ (Piwi-Argonaute-Zwille) domain, two RNase III domains, and a double-stranded RNA binding domain; however, the domain composition of Dicers varies among species. Dicer and its homologues developed only in eukaryotes; nevertheless, the two enzymatic domains of Dicer, helicase and RNase III, display high sequence similarity to their prokaryotic orthologs. Evolutionary studies indicate that a combination of the helicase and RNase III domains in a single protein is a eukaryotic signature and is supposed to be one of the critical events that triggered the consolidation of the eukaryotic RNA interference. In this review, we provide the genetic insight into the domain organization and structure of Dicer proteins found in vertebrate and invertebrate animals, plants and fungi. We also discuss, in the context of the individual domains, domain deletion variants and partner proteins, a variety of Dicers’ functions not only related to small RNA biogenesis pathways.     

Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots

Extracellular ATP (eATP) has long been established in animals as an important signalling molecule but this is less understood in plants. The identification of Arabidopsis thaliana DORN1 (Does Not Respond to Nucleotides) as the first plant eATP receptor has shown that it is fundamental to the elevation of cytosolic free Ca²⁺ ([Ca²⁺]_cyt) as a possible second messenger. eATP causes other downstream responses such as increase in reactive oxygen species (ROS) and nitric oxide, plus changes in gene expression. The plasma membrane Ca²⁺ influx channels involved in eATP-induced [Ca²⁺]_cyt increase remain unknown at the genetic level. Arabidopsis thaliana Annexin 1 has been found to mediate ROS-activated Ca²⁺ influx in root epidermis, consistent with its operating as a transport pathway. In this study, the loss of function Annexin 1 mutant was found to have impaired [Ca²⁺]_cyt elevation in roots in response to eATP or eADP. Additionally, this annexin was implicated in modulating eATP-induced intracellular ROS accumulation in roots as well as expression of eATP-responsive genes.     

Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice

Salivary gland dysfunction induces salivary flow reduction and a dry mouth, and commonly involves oral dysfunction, tooth structure deterioration, and infection through reduced salivation. This study aimed to investigate the impact of aging on the salivary gland by a metabolomics approach in an extensive aging mouse model, SAMP1/Klotho -/- mice. We found that the salivary secretion of SAMP1/Klotho -/- mice was dramatically decreased compared with that of SAMP1/Klotho WT (+/+) mice. Metabolomics profiling analysis showed that the level of acetylcholine was significantly decreased in SAMP1/Klotho -/- mice, although the corresponding levels of acetylcholine precursors, acetyl-CoA and choline, increased. Interestingly, the mRNA and protein expression of choline acetyltransferase (ChAT), which is responsible for catalyzing acetylcholine synthesis, was significantly decreased in SAMP1/Klotho -/- mice. The overexpression of ChAT induced the expression of salivary gland functional markers (α–amylase, ZO-1, and Aqua5) in primary cultured salivary gland cells from SAMP1/Klotho +/+ and -/- mice. In an in vivo study, adeno-associated virus (AAV)-ChAT transduction significantly increased saliva secretion compared with the control in SAMP1/Klotho -/- mice. These results suggest that the dysfunction in acetylcholine biosynthesis induced by ChAT reduction may cause impaired salivary gland function