Mechanical Load and Piezo1 Channel Regulated Myosin II Activity in Mouse Lenses

The cytoarchitecture and tensile characteristics of ocular lenses play a crucial role in maintaining their transparency and deformability, respectively, which are properties required for the light focusing function of ocular lens. Calcium-dependent myosin-II-regulated contractile characteristics and mechanosensitive ion channel activities are presumed to influence lens shape change and clarity. Here, we investigated the effects of load-induced force and the activity of Piezo channels on mouse lens myosin II activity. Expression of the Piezo1 channel was evident in the mouse lens based on immunoblot and immufluorescence analyses and with the use of a Piezo1^-tdT transgenic mouse model. Under ex vivo conditions, change in lens shape induced by the load decreased myosin light chain (MLC) phosphorylation. While the activation of Piezo1 by Yoda1 for one hour led to an increase in the levels of phosphorylated MLC, Yoda1 treatment for an extended period led to opacification in association with increased calpain activity and degradation of membrane proteins in ex vivo mouse lenses. In contrast, inhibition of Piezo1 by GsMTx4 decreased MLC phosphorylation but did not affect the lens tensile properties. This exploratory study reveals a role for the mechanical load and Piezo1 channel activity in the regulation of myosin II activity in lens, which could be relevant to lens shape change during accommodation.     

Cytopathological Outcomes of Knocking down Expression of Mitochondrial Complex II Subunits in Dictyostelium discoideum

Mitochondrial Complex II is composed of four core subunits and mutations to any of the subunits result in lowered Complex II activity. Surprisingly, although mutations in any of the subunits can yield similar clinical outcomes, there are distinct differences in the patterns of clinical disease most commonly associated with mutations in different subunits. Thus, mutations to the SdhA subunit most often result in mitochondrial disease phenotypes, whilst mutations to the other subunits SdhB-D more commonly result in tumour formation. The reason the clinical outcomes are so different is unknown. Here, we individually antisense-inhibited three of the Complex II subunits, SdhA, SdhB or SdhC, in the simple model organism Dictyostelium discoideum. Whilst SdhB and SdhC knockdown resulted in growth defects on bacterial lawns, antisense inhibition of SdhA expression resulted in a different pattern of phenotypic defects, including impairments of growth in liquid medium, enhanced intracellular proliferation of the bacterial pathogen Legionella pneumophila and phagocytosis. Knockdown of the individual subunits also produced different abnormalities in mitochondrial function with only SdhA knockdown resulting in broad mitochondrial dysfunction. Furthermore, these defects were shown to be mediated by the chronic activation of the cellular energy sensor AMP-activated protein kinase. Our results are in agreement with a role for loss of function of SdhA but not the other Complex II subunits in impairing mitochondrial oxidative phosphorylation and they suggest a role for AMP-activated protein kinase in mediating the cytopathological outcomes.     

2^N-1级联式30电平逆变器

本文介绍了一种新型的2^N-1级联式30电平逆变器的基本工作原理和控制方法。     

浅谈地源热泵在医院建筑中的应用

随着人们保健意识的日益提高,人们自然对医疗环境的要求也相应提高,对于现代化医疗建筑建设者来讲,营造生态保健型绿色医院就成为我们的努力方向,在现代医院建筑中引入绿色节能的地源热泵技术,使医院真正成为人类和谐舒适,环境优美,绿色生态的现代化疗养场所。因此,地源热泵系统在现代医院建筑中具有很大的应用价值,它将成为我国医院建筑节能领域重点推广的技术之一。     

Going from green to white color electroluminescence through a nanoscale complex of Zinc (II)

In this paper, we report application of four novel Nano Zinc (II) complexes as the emitter dyes in organic light emitting diodes (OLEDs). The Nano Zinc (II) complexes emission, particularly its absorption, fluorescence spectra and quantum yield, tuned by varying sizes which affects environments of the Zinc (II) ion. OLED Devices with Nano Zinc (II) complexes were fabricated, giving rise to devices with peak emission ranging from 498 to 541 nm. Here, we have successfully employed sonoelectrochemical method for fabricating white OLEDs. The white emission ascribed to the exciton emission in Nano Zinc (II) complex emitter and from exciplex formation at the interface of PVK/Nano Zinc (II) complex. The experimental results demonstrate that this approach is ideal for color tuning of single and multilayer conducting semiconducting thin films used in the fabrication of organic electronic devices such as OLEDs.     

数字化医院信息安全建设与管理策略分析

在医疗体制改革中,信息技术被广泛地运用,推动医院数字化建设.在医院数字化建设中,信息安全建设占据重要地位,是实现医院现代化发展的重要组成部分.重视医院信息安全建设与管理工作,对减少医院医疗事故发生频率、提升病患就诊效率等有重要意义,可实现医院数字化建设,实现医院工作流程简化,进而提升医院工作效率.文章阐述数字化医院信息...     

大型医院内部网络系统集成的设计与应用

文中叙述了一个大型医院内部网络系统集成的设计方案,指出了网络总体结构设计、网络路由设计、网络安全设计是医院网络系统集成建设的重中之重,并给出了这三个部分的具体设计方案.最后给出了网络拓扑图及具体设备选型及实施方案.     

JTAG测试技术在模块级测试中的应用

目前,随着板级器件密度的不断增大,尤其是表贴器件、BGA器件的大量使用,传统的测试方法,如使用万用表、示波器等已不能够覆盖足够的测试面积,这为模块测试带来了挑战。而随着支持IEEE1149.1标准的器件在模块中广泛的应用,很好的解决了这一问题。文章从JTAG边界扫描技术的原理出发,探讨如何将该技术应用到模块级测试中,为模块测试诊断带来便利。