Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, {"type":"entrez-protein","attrs":{"text":"AAV27984.1","term_id":"53987783","term_text":"AAV27984.1"}}AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.     

A Study of an Intelligent Battlefield Damage Assessment System Based on a Multi-agent System

Battlefield damage assessment is the key to Battlefield Damage Assessment and Repair (BDAR).We present an Intelligent Battlefield Damage Assessment System (IBDAS) based on multi-agent system technology. We first establish the system framework, and then study the interior structure and workflow of a problem allocation agent. The result shows that, there are many advantages to resolve the problem of battlefield damage assessment by applying multi-agent system technology, and it will bring significant military benefit.     

基于神经网络的长距离油气管道安全预警系统

提出了一种基于分布式光纤传感和人工神经网络判别的长距离输油管道安全预警系统.该系统利用光纤传感器收集管道周围土壤的振动信号,通过神经网络判断是否存在针对管道的破坏性行为和判别破坏性行为的类别,实现对油气管道的长距离安全预警.系统在预处理阶段对信号大幅度降维,降低数据处理的时间复杂度,以满足实时性的要求.在识别阶段则采用人工神经网络模型,包括反向传播(BP)网络和支持向量机(SVM).试验结果表明,这两种神经网络模型对打夯、镐刨、电钻三类破坏行为的识别率分别达到96.5和97.1%,均优于以往文献中的报道.     

城市库废放射源治理工作中的剂量控制

城市放射性废物库废放射源治理过程中影响工作人员受照剂量的因素较多。为实现辐射防护的最优化,本文以全国城市库治理项目统计结果及现场实施经验为依据,分析了城市库废源的特点、辐射风险及剂量控制方法。并对城市库废源治理项目的剂量数据进行统计分析,发现剂量分布规律,为辐射防护工作提供指导和参考。     

基于暂态零模电流的配电网故障区段定位

为降低小电流接地系统通信过程中小波扰动(噪声)对暂态电流法的干扰,减小因采样频率增加造成的通信网络负担,解决故障两侧馈线终端不同步的问题,提出了一种小电流接地系统的故障区段定位方法.通过检测故障区段两侧的暂态零模电流,提取其中的极值,并计算其衰减程度,利用其极性进行辅助定位.数值仿真的结果验证了所提方法的有效性,该方法...     

太赫兹波辐射源的研究进展

电磁波谱中的太赫兹波段拥有许多比传统光源更独特的性质,在生物、材料、安检和通信等领域有着非常重要的研究价值,而高功率和高效率的太赫兹辐射源则是太赫兹技术转化为实际生产力的一个重要因素和前提条件.文章分别从以气体、固体和液体作为激发介质的角度,总结了激光与不同激发介质相互作用产生太赫兹波的研究进展.对气体介质中常用的四波...     

徐深气田火山岩储层岩性与流体性质测井识别研究

在充分考虑火山岩储层特征和地层非均质性的基础上,运用常规测井信息、气测录井及测井新技术对火山岩储层进行综合评价。应用岩心观察、薄片分析、扫描电镜等资料,采用交会图法、主成份分析等方法,对火山岩储层进行了岩性识别;应用试气、测井、气测录井等资料对储层流体性质进行了判别。     

感应测井的最小平方反滤波

感应测井响应是地层非线性时变滤波的结果。获得地层真电导率的方法就是将感应测井响应进行非线性时变的反滤波处理。本文用最小平方法设计出了一种非线性的反滤波因子,并对模拟的感应测井响应曲线进行了处理。结果表明,与传统的处理方法(包括三因子反滤波和趋肤效应提升)相比,该方法可大大地改进感应测井的精度。     

高密度钻井液随钻堵漏技术研究

地层和油气层分布的特点，使塔里木盆地易发生高密度条件下的井漏。多种堵漏处理措施，如降排量、降密度、静止堵漏、随钻堵漏、桥接堵漏、注水泥堵漏等都没有很好地解决这类漏失问题。根据井漏及堵漏情况，开发出了高密度条件下使用的随钻封堵护壁剂。该堵漏剂主要以改性纤维作为骨架材料，改性沥青和天然有机高分子作为填充材料，以弱交联聚合物为变形填充材料，并辅以有机硅为粘结护壁材料，是一种具有物理化学堵漏功能的新型随钻堵漏剂。经流变性、砂床滤失性、润滑性、油层保护性等性能评价，表明该处理剂加入后，对钻井液流变性影响小、可辅助降低API滤失量和润滑系教、能碴著降低砂床滤失鼍。同时还具有一定的保护油气层的功能。     

大港油田水平井保护油层钻井液技术

水平井油层保护工作比普通开发井更为重要,其重要环节是钻井液的油层保护工作。大港油田集团钻井泥浆技术服务公司针对大港油田和冀东油田断块型砂岩油层特点,依据不同的完井方式,研究出适应不同区块、不同完井方式的水平井钻井液保护油层方案,在75口水平井进行了现场应用,取得了较好的油层保护效果,平均渗透率恢复值达到86.75%,试验井原油产量均超过配产指标。现场应用结果表明,广谱型屏蔽暂堵和理想充填保护油气层技术能实现有效暂堵,确保封堵效果,非常适用于非均质砂岩油藏的油层保护,其中理想充填保护油气层技术是借助计算机软件工作,操作更方便,它更适用于高孔高渗的砂岩油藏;低固相超低渗透保护油气层钻井液技术适应筛管完井的水平井油层保护,具有较高的现场应用价值;油层保护研究必须按区块、分层位进行,油层保护方案必须个性化,才能取得理想的效果。