全文获取类型
收费全文 | 97篇 |
免费 | 19篇 |
专业分类
综合类 | 1篇 |
化学工业 | 14篇 |
机械仪表 | 8篇 |
建筑科学 | 2篇 |
轻工业 | 36篇 |
水利工程 | 1篇 |
无线电 | 1篇 |
一般工业技术 | 4篇 |
原子能技术 | 8篇 |
自动化技术 | 41篇 |
出版年
2024年 | 1篇 |
2022年 | 2篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 11篇 |
2016年 | 5篇 |
2015年 | 14篇 |
2014年 | 9篇 |
2013年 | 2篇 |
2012年 | 8篇 |
2011年 | 10篇 |
2010年 | 11篇 |
2009年 | 11篇 |
2008年 | 7篇 |
2007年 | 5篇 |
2006年 | 1篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 1篇 |
2001年 | 1篇 |
排序方式: 共有116条查询结果,搜索用时 15 毫秒
1.
2.
3.
Karlsson H Mörtstedt H Lindqvist H Tagesson C Lindahl M 《Proteomics. Clinical applications》2009,3(6):663-671
Although obesity and high levels of low-density lipoprotein (LDL) are well-known risk factors for cardiovascular disease, the precise role(s) of different LDL constituents in obesity has not been explored. In the present study, we compared the LDL proteome of healthy control adults (body mass index<25) and obese subjects (body mass index>30). LDL was isolated by density-gradient ultracentrifugation and proteins were separated with 2-D PAGE, quantified, and identified by peptide mass fingerprinting using MALDI-TOF MS. A new LDL-associated protein was identified as transthyretin and found to be significantly more abundant in LDL from the obese subjects. In addition, LDL from the obese subjects contained relatively more α(1) -antitrypsin, apo J, apo C-II, than LDL from controls, and also more of an acidic isoform (pI/Mr; 5.2/23 100) of apo A-I. On the other hand, the relative amounts of apo A-IV and the major isoform of apo A-I (pI/Mr; 5.3/23 100) were significantly less in LDL from the obese subjects. Apo E was less and non-sialylated apo C-III more abundant in LDL from obese men than control men, while there were no such differences between LDL from obese and control women. These findings illustrate that obesity is not only associated with increased LDL-cholesterol levels but also with alterations in the LDL protein composition. The presence of transthyretin in LDL from obese subjects may reflect over-nutrition and affect the lipid metabolism in obesity. 相似文献
4.
5.
应用蛋白质组学方法筛选对低剂量?射线敏感的蛋白质,并采用双向凝胶电泳和基质辅助激光解析飞行时间串联质谱技术分离、鉴定不同低剂量?射线导致的小鼠血清差异表达蛋白。不同低剂量?射线照射后,对实验组与对照组的血清双向电泳图谱进行比较分析,通过质谱鉴定得到7个差异表达点,分别是载脂蛋白C-III、?珠蛋白、腮腺分泌蛋白、?2巨球蛋白前体、转甲状腺蛋白晶体结构H链、C1qc蛋白和丛生蛋白。提示低剂量?射线辐照能导致小鼠血清中某些蛋白表达发生变化。 相似文献
6.
Li F Seillier-Moiseiwitsch F Korostyshevskiy VR 《Computational statistics & data analysis》2011,55(11):3059-3072
A new comprehensive procedure for statistical analysis of two-dimensional polyacrylamide gel electrophoresis (2D PAGE) images is proposed, including protein region quantification, normalization and statistical analysis. Protein regions are defined by the master watershed map that is obtained from the mean gel. By working with these protein regions, the approach bypasses the current bottleneck in the analysis of 2D PAGE images: it does not require spot matching. Background correction is implemented in each protein region by local segmentation. Two-dimensional locally weighted smoothing (LOESS) is proposed to remove any systematic bias after quantification of protein regions. Proteins are separated into mutually independent sets based on detected correlations, and a multivariate analysis is used on each set to detect the group effect. A strategy for multiple hypothesis testing based on this multivariate approach combined with the usual Benjamini-Hochberg FDR procedure is formulated and applied to the differential analysis of 2D PAGE images. Each step in the analytical protocol is demonstrated by using an actual dataset. The effectiveness of the proposed methodology is shown using simulated gels in comparison with the commercial software packages PDQuest and Dymension. We also introduce a new procedure for simulating gel images. 相似文献
7.
Search for novel circulating cancer chemopreventive biomarkers of dietary rice bran intervention in ApcMin mice model of colorectal carcinogenesis,using proteomic and metabolic profiling strategies
下载免费PDF全文
![点击此处可从《Molecular nutrition & food research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
8.
9.
10.
Priscila Camillo Teixeira Philippe Ferber Nicolas Vuilleumier Paul Cutler 《Proteomics. Clinical applications》2015,9(1-2):48-57
Autoimmune diseases, such as antiphospholipid syndrome, systemic lupus erythematosus, and rheumatoid arthritis, are characterized by a high prevalence of cardiovascular (CV) disease (CVD), which constitutes the leading causes of morbidity and mortality among such patients. Although such effects are partly explained by a higher prevalence of traditional CV risk factors, many studies indicate that such factors do not fully explain the enhanced CV risk in these patients. In addition, risk stratification algorithms based upon traditional CV risk factors are not as predictive in autoimmune diseases as in the general population. For these reasons, the timely and accurate assessment of CV risk in these high-risk populations still remains an unmet clinical need. An enhanced contribution of different inflammatory components of the immune response, as well as autoimmune elements (e.g. autoantibodies, autoantigens, and cellular response), has been proposed to underlie the incremental CV risk observed in these populations. Recent advances in proteomic tools have contributed to the discovery of proteins involved in CVDs, including some that may be suitable to be used as biological markers. In this review we summarize the main markers in the field of CVDs associated with autoimmunity, as well as the recent advances in proteomic technology and their application for biomarker discovery in autoimmune disease. 相似文献