EVALUATION OF MALTING QUALITY IN A BARLEY BREEDING PROGRAMME USE OF α-AMYLASE AND β-GLUCAN LEVLES IN MALT AS PRESELECTION TOOLS

Analysis according to the EBC protocol, immunological determination of a α-amylase and estimation of malt β-glucan using the Calcofluor-FIA method, were used to screen 327 barley breeding lines for malting quality. The results obtained with the α-amylase and β-glucan methods are highly correlated to the important malt quality paramters: extract yield and β-glucan content in the wort. It is recommended that either of the two methods, which are simple to perform are used as prescreening tools in breeding programmes for malting barley.     

STARCH DEGRADATION IN ENDOSPERMS OF DEVELOPING BARLEY KERNELS*

Shortly after anthesis, empty endosperm cells adjacent to the embryo were crushed between the developing embryo and endosperm tissues to form the crushed cell layer. Starch granules in cells adjacent to this layer were hydrolysed and the empty cells were added to the crushed layer. In this way, the crushed layer increased in thickness throughout kernel development. α-Amylase 2 was detected in the crushed cell layer region of barley endosperms during the period that starch granule hydrolysis was occurring.     

THE CONTRIBUTION OF DEXTRINS OF BEER SENSORY PROPERTIES PART II. AFTERTASTE

We investigated the possibility that dextrins could contribute to the aftertaste of beer by being broken down to sweet sugars by salivary α-amylase after beer Ingestion. The volume, pH and temperature of beer and saliva present in the oral cavity after swallowing were measured on several subjects and found adequate for the hydrolysis of beer dextrins by salivary amylase. The in vitro hydrolysis of a 6% dextrin solution and of commercial beers by pancreatic α-amylase yielded significant amounts of glucose and maltose in addition to maltotriose. Similarly, the hydrolysis of commercial beers by human saliva produced between 2.7 and 7.4 g/litre of sweet-tasting maltose and glucose, depending on the duration of the hydrolysis (30 sec to 2 min) and the amount of saliva added to 2.5 ml of beer (0.5 or 2 ml). This much sugar, because it is produced over a period of time, may not cause a detectable sweet taste but it might partially mask the bitter aftertaste of beer.     

Transparent,Highly Stretchable,Rehealable, Sensing,and Fully Recyclable Ionic Conductors Fabricated by One‐Step Polymerization Based on a Small Biological Molecule

To date, various stretchable conductors have been fabricated, but simultaneous realization of the transparency, high stretchability, electrical conductivity, self‐healing capability, and sensing property through a simple, fast, cost‐efficient approach is still challenging. Here, α‐lipoic acid (LA), a naturally small biological molecule found in humans and animals, is used to fabricate transparent (>85%), electrical conductivity, highly stretchable (strain up to 1100%), and rehealable (mechanical healing efficiency of 86%, electrical healing efficiency of 96%) ionic conductor by solvent‐free one‐step polymerization. Furthermore, the ionic conductors with appealing sensitivity can be served as strain sensors to detect and distinguish various human activities. Notably, this ionic conductor can be fully recycled and reprocessed into new ionic conductors or adhesives by a direct heating process, which offers a promising prospect in great reduction of electronic wastes that have brought acute environmental pollution. In consideration of the extremely facile preparation process, biological available materials, satisfactory functionalities, and full recyclability, the emergence of LA‐based ionic conductors is believed to open up a new avenue for developing sustainable and wearable electronic devices in the future.     

高g值高频响微机械加速度计研究

针对侵彻武器测试过程中冲击过载大和频率范围宽的特点,提出了一种微机械电容式加速度计结构,结构中采用分布在整个芯片上的小电容阵列来代替位于中间的单个大电容器.利用ANSYS对加速度计进行了仿真分析,得出加速度计的固有频率超过600 kHz,抗过载能力超过200 000 g,且电容变化量大,灵敏度高.文中还给出了加速度计芯片的加工工艺流程和接口电路.     

模拟乘法器MC1496的应用研究

模拟乘法器是一种完成两个模拟信号相乘的电子器件。本文基于电路设计与仿真软件Multisim进行模拟乘法器MC1496的应用研究,具体实现普通调幅（AM）、双边带调幅（DSB）、同步检波器、混频器的电路设计与仿真。     

红外无线数字通信

本文介绍了计算机领域正在兴起的一种通信技术－－红外无线数字通信，介绍了其工作原理及组成结构，目前已有的两个标准：ＩｒＤＡ及ＩＥＥＥ８０２．１１。对国外已有的产品及专用ＩＣ进行了总结，并就国外的一些研究成果作了简要综述。     

微带补片激励的宽带背腔天线

为满足飞行器天线在防护层下工作的要求，从双层微带天线出发，采用ANSOFTHFSS软件计算了天线窗内的场分布；阐述了微带激励的背腔天线的工作模式，包含微带TM11模过渡为口径面近似TE11模辐射和开口的异形谐振腔（TM010和TM210）辐射；设计出了宽带匹配的天线样机，并给出了相关设计尺寸。测试结果表明，该天线在S波段驻波比（Voltage Standing Wave Ratio，VSWR）≤2．0的匹配带宽达到42％。     

Ice‐Assisted Synthesis of Black Phosphorus Nanosheets as a Metal‐Free Photocatalyst: 2D/2D Heterostructure for Broadband H2 Evolution

A 2D/2D heterojunction of black phosphorous (BP)/graphitic carbon nitride (g‐C₃N₄) is designed and synthesized for photocatalytic H₂ evolution. The ice‐assisted exfoliation method developed herein for preparing BP nanosheets from bulk BP, leads to high yield of few‐layer BP nanosheets (≈6 layers on average) with large lateral size at reduced duration and power for liquid exfoliation. The combination of BP with g‐C₃N₄ protects BP from oxidation and contributes to enhanced activity both under λ > 420 nm and λ > 475 nm light irradiation and to long‐term stability. The H₂ production rate of BP/g‐C₃N₄ (384.17 µmol g^?1 h^?1) is comparable to, and even surpasses that of the previously reported, precious metal‐loaded photocatalyst under λ > 420 nm light. The efficient charge transfer between BP and g‐C₃N₄ (likely due to formed N? P bonds) and broadened photon absorption (supported both experimentally and theoretically) contribute to the excellent photocatalytic performance. The possible mechanisms of H₂ evolution under various forms of light irradiation is unveiled. This work presents a novel, facile method to prepare 2D nanomaterials and provides a successful paradigm for the design of metal‐free photocatalysts with improved charge‐carrier dynamics for renewable energy conversion.     

Analysis of the peroxisomal acyl‐CoA oxidase gene product from Pichia pastoris and determination of its targeting signal

Acyl‐CoA oxidase (Pox1p) is involved in the β‐oxidation of fatty acids and is targeted to the peroxisomal matrix via the use of different signals in various organisms. In rat, mouse and human, Pox1p contains a canonical peroxisomal targeting signal 1 (PTS1), whereas in the yeasts Candida tropicalis, Saccharomyces cerevisiae, C. maltosa and Yarrowia lipolytica neither a PTS1 nor a PTS2 sequence is present, suggesting that Pox1p might be targeted to the peroxisomes via a third unknown pathway. Alternatively, since proteins lacking a PTS sequence can enter peroxisomes in association with other polypeptides containing a PTS, Pox1p might ‘piggy‐back’ its way into the peroxisomal matrix together with other proteins. To understand the mechanism of peroxisomal targeting of a yeast Pox1p, we cloned the Pichia pastoris POX1 gene to study the pathway of import of PpPox1p into peroxisomes. The gene was cloned by PCR, hybridization and plasmid rescue. The 2157 bp gene encodes a protein with a predicted molecular weight of 80 kDa. Antisera against PpPox1p detected a protein specifically induced on oleate with an apparent molecular weight of 72 kDa. Immunolocalization studies confirmed the peroxisomal localization of PpPox1p. The carboxy‐terminus of PpPox1p ends with a PTS1‐like sequence, APKI. The sequence PKI was necessary for transport of PpPox1p into peroxisomes and interacted with the PTS1 receptor, Pex5p. Furthermore, addition of the sequence APKI to the C‐terminus of the green fluorescent protein directed this fusion protein to the peroxisome. Therefore, PpPox1p uses the PTS1 pathway for its import into peroxisomes. Copyright © 1999 John Wiley & Sons, Ltd.