不同破壁方法提取绍兴黄酒麦曲中的微生物总DNA

分别采用超声波、Yatalase酶法、氯化苄法对黄酒麦曲样品进行破壁,提取微生物总DNA,并通过细胞裂解率、DNA的纯度、片段的分布情况、ITS(内转录间隔区)序列扩增产物的多态性来评估不同的破壁方法对麦曲样品总DNA提取效果的影响。实验结果表明,采用氯化苄法能裂解86%的孢子,OD260/OD280在1.5左右,所得DNA片段分布在100bp～20kbI,TS-PCR扩增出的条带最多,而且简单、经济、提取效果好,可以作为麦曲中微生物总DNA的提取方法。     

多重PCR技术在食源性致病菌检测中应用的研究进展

食源性致病菌的多重PCR检测技术是指能够同时扩增得到同种或多种致病菌的不同基因片段的技术。因该技术特异、灵敏且分析效率高,现已被广泛应用于食源性致病菌的检测工作中。本文介绍了多重PCR在食源性致病菌检测中应用的研究近况,并对影响因素及存在的问题进行了阐述。      

染整业电子供应链管理系统研究

介绍了染整业的现状以及电子供应链管理的基本原理和实现技术，从J2EE平台体系结构、移植性、扩展性等多个角度对J2EE平台进行了相应的分析，提出了一个针对染整业的基于J2EE平台的电子供应链管理系统框架，并对其可行性作了具体说明。     

A novel real-time polymerase chain reaction method for the detection of macadamia nuts in food

A real-time PCR-based method for the detection of macadamia nuts (fruits of Macadamia integrifolia or M. tetraphylla or their hybrids) in food products is described. The method consists of DNA isolation by chaotropic solid phase extraction and subsequent PCR with macadamia-specific primers and a TaqMan fluorescent probe. The primers and the probe were targeted to the gene encoding for vicilin precursor. The method was positive for M. integrifolia and M. tetraphylla and negative for 16 other plant species used in food industry, including peanuts, walnuts, hazelnuts, almonds, pistachio nuts, cashew nuts, Brazil nuts, and chestnuts. The DNA-based detection limit of the method was 1.45 pg. Using a series of model samples with defined macadamia nut contents, a practical detection limit of 0.02% (w/w) macadamia nuts was determined. Practical applicability of the PCR method was tested by the analysis of 14 confectionery samples. For all of the samples, results conforming to the labeling were obtained. The presented PCR method is useful for relatively fast, highly selective, and moderately sensitive detection of macadamia nuts in food samples.     

纱线张力检测与控制技术的研究现状与展望

总结纱线张力检测与控制技术的研究现状及发展趋势.阐述了纱线"三辊式"张力检测的原理,对当前国内外有关接触式和非接触式纱线张力检测及控制原理的研究状况进行调研与分析.指出:当前纱线张力检测和控制技术的研究主要方向为采用非接触方式动态测试纱线张力并采取自适应控制技术,以使纱线的张力保持恒定.研究主要集中在提高测试系统的精度、性能、使用方便性及环境适应性上.其发展的总趋势是拓展测量范围,提高检测精度和可靠性;采用闭环控制技术,实现恒张力控制;采用微机电技术,实现在线非接触式检测与控制.     

四川家具产业集群的价值链升级

四川家具产业集群已形成了一定规模和特色,并依托国内大市场走出了与沿海家具产业发展不同的路线。但在规模优势之外．四川家具产业需要科学规划产业集群价值链的升级,从而推动包括产品．企业、产业的整体升级。     

食品供应链中的肉品安全管理——从农场饲养到屠宰环节

我国肉制品质量与安全问题日益突出,如何在供应链的各个环节,采取措施保障肉品安全管理体系,具有现实的意义。构建肉类食品的原料安全生产系统、全面质量管理系统、溯源管理系统等安全保障措施是构成肉类食品安全保障体系的重点。并且本文从提高中国肉类加工食品的市场竞争力出发,针对目前中国肉类加工食品尚未形成完善的供应链管理模式这一现状,提出了在新型的肉类供应链安全管理模型,旨在推进我国肉类工业健康稳步发展。     

Oxidative stability of palm‐ and soybean‐based medium‐ and long‐chain triacylglycerol (MLCT) oil blends

BACKGROUND: Medium‐ and long‐chain triacylglyerols (MLCT) enzymatically esterified using Lipozyme RM IM lipase has very low oxidative stability as it does not contain any antioxidants. The aim of this work was to study the ability of various antioxidants to increase the oxidative stability of palm‐ and soybean‐based MLCT blends which assist to bring up the oxidative stability of both MLCT blends. In this study, the effectiveness of rosemary extracts, sage extracts, tert‐butylhydroquinone (TBHQ) and mixtures of tert‐butyl‐4‐hydroxyanisole (BHA) and tert‐butyl‐p‐hydroxytoluene (BHT) in protecting against oxidation of various MLCT blends was investigated. RESULTS: Blending of MLCT oil with either palm olein or soybean oil improved its smoke point values and oxidative stability. TBHQ addition to both palm‐ and soybean‐based MLCT blends increased oxidative stability. Combination of BHA and BHT showed no significant improvement (P > 0.05) in ability to protect blends from oxidation compared to natural antioxidants such as sage or rosemary extracts. CONCLUSION: Blended oils with 500 g kg^?1 MLCT and 500 g kg^?1 palm olein (MP5) were the most suitable for use at high temperature based on the fatty acid composition of the MLCT blends, which subsequently had an effect on thermal oxidative stability. In general, addition of either natural or synthetic antioxidant assisted in improving the antioxidative strength of both MLCT blends. MLCT blends with added TBHQ showed the highest thermal oxidative stability among the antioxidants used. Copyright © 2008 Society of Chemical Industry     

Integration of enterprise levels based on an ontological framework

Enterprises are highly complex systems in which one or more organizations share a definite mission, goals and objectives to offer a product or service. In this study, an ontological framework is built as a mechanism for exchanging information and knowledge models for multiple applications and effective integration between hierarchical levels. The potential of the general semantic framework that is developed is demonstrated using a case study concerning the enterprise supply chain network design-planning problem.     

Molecular thermodynamics of binary polymer solutions using modified double lattice model: Chain length dependence of primary lattice

In a previous study we modified a double lattice model by introducing a new interaction parameter, which improved the mathematical approximation defect, and gave a new expression for the Helmholtz energy of mixing. In the model the universal constants C_β and C_γ in the primary lattice were determined by comparing them with literature Monte Carlo simulation data, which is the only case for r₁ = 1 and r₂ = 100 (case I). In this study we introduce new universal constants, C_β and C_γ, as a function of the chain length of a polymer in a solvent (case II) by comparing them with other literature simulation data for various polymer chain lengths. The proposed model is compared with polymer–solvent systems. In an upper critical solution temperature phase behavior the theoretical results of case II were improved over those of case I. However, in a lower critical solution temperature phase behavior those of case I were not very sensitive to C_β and C_γ. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 73: 2627–2633, 1999