WiNoC中基于Edge first算法的流量平衡设计

在无线片上网络中,无线节点拥塞以及不同子网和全局网络内的流量平衡情况对整个片上网络的通信效率有着重要的影响,为此提出了基于Edge first算法的全局流量平衡机制（GTB）。首先优化了划分有线无线数据包的机制,减少了无线节点处的拥塞;其次根据无线路由器（WR）的拥塞情况,提出Edge first路由算法平衡子网内的流量;最后在全局网络中提出了全局子网拥塞感知（GSCA）判断机制,使得长距离数据包优先从低拥塞子网通过,平衡了全局网络的流量。实验表明,该方案在可接受的硬件开销、功耗开销下,保证较低的网络延迟和较高的网络吞吐率,并且大幅的提升了网络的流量平衡性能。     

Structural Basis for The Recognition of Deaminated Nucleobases by An Archaeal DNA Polymerase

With increasing temperature, nucleobases in DNA become increasingly damaged by hydrolysis of exocyclic amines. The most prominent damage includes the conversion of cytosine to uracil and adenine to hypoxanthine. These damages are mutagenic and put the integrity of the genome at risk if not repaired appropriately. Several archaea live at elevated temperatures and thus, are exposed to a higher risk of deamination. Earlier studies have shown that DNA polymerases of archaea have the property of sensing deaminated nucleobases in the DNA template and thereby stalling the DNA synthesis during DNA replication providing another layer of DNA damage recognition and repair. However, the structural basis of uracil and hypoxanthine sensing by archaeal B-family DNA polymerases is sparse. Here we report on three new crystal structures of the archaeal B-family DNA polymerase from Thermococcus kodakarensis (KOD) DNA polymerase in complex with primer and template strands that have extended single stranded DNA template 5’-overhangs. These overhangs contain either the canonical nucleobases as well as uracil or hypoxanthine, respectively, and provide unprecedented structural insights into their recognition by archaeal B-family DNA polymerases.     

Efficient Linear dsDNA Tagging Using Deoxyuridine Excision**

Site-specific strategies for exchanging segments of dsDNA are important for DNA library construction and molecular tagging. Deoxyuridine (dU) excision is an approach for generating 3’ ssDNA overhangs in gene assembly and molecular cloning procedures. Unlike approaches that use a multi-base pair motif to specify a DNA cut site, dU excision requires only a dT→dU substitution. Consequently, excision sites can be embedded in biologically active DNA sequences by placing dU substitutions at non-perturbative positions. In this work, I describe a molecular tagging method that uses dU excision to exchange a segment of a dsDNA strand with a long synthetic oligonucleotide. The core workflow of this method, called deoxyUridine eXcision-tagging (dUX-tagging), is an efficient one-pot reaction: strategically positioned dU nucleotides are excised from dsDNA to generate a 3’ overhang so that additional sequence can be appended by annealing and ligating a tagging oligonucleotide. The tagged DNA is then processed by one of two procedures to fill the 5’ overhang and remove excess tagging oligo. To facilitate its widespread use, all dUX-tagging procedures exclusively use commercially available reagents. As a result, dUX-tagging is a concise and easily implemented approach for high-efficiency linear dsDNA tagging.     

基于DSP芯片的斜齿轮插齿数控加工技术研究

国内斜齿轮加工一般采用滚齿机或者进口插齿机,由于产品体积过大和机床价格昂贵而受到限制.文章对采用DSP芯片的数控插齿加工技术进行了研究,提出了虚拟轴插补技术以及用软件实现电子齿轮差动技术,利用DSP芯片的高速处理能力进行跟踪插补,经初步试验证明该方法理论上可行,实际中可以运用.     

X6125铣床数控化改造

通过对我院教学实习基地的一台X6125铣床结构和性能的研究，针对盘式凸轮加工的特点，并结合现有条件，确定了铣床的数控化改造方案，介绍了滚珠丝杠、步进电动机的选择设计以及数控系统软硬件的设计。改造后的铣床可提高生产效率，保证产品质量和减轻工人的劳动强度。     

多表位DNA壳聚糖微球疫苗的体液免疫应答

目的研究多表位DNA壳聚糖微球疫苗的体液免疫应答。方法制备多表位DNA壳聚糖微球疫苗pcD-NA3.1-HME-3C3d,经鼻腔免疫小鼠,蛋白检测微孔试剂盒检测小鼠特异性IgG抗体水平。结果经免疫的小鼠均能产生针对各表位的特异性IgG抗体,DNA壳聚糖微球疫苗的抗体水平明显高于DNA疫苗。结论壳聚糖微球疫苗投递系统可提高多表位DNA疫苗的免疫应答效果。     

基于单片机的PID温控系统在塑料挤出机中的应用

针对在塑料挤出过程中温度控制的重要性，介绍了一种新型的PID温度控制系统。该系统采用高性能单片机芯片，可以方便地对系统加热、冷却周期及PID的各个参数进行在线设定和修改，提高了塑料挤出机的温控精度。     

聚甲基丙烯酰氧乙基二甲基丁烷基溴化铵共振光散射法测定核酸

通过光谱法研究聚甲基丙烯酰氧乙基二甲基丁烷基溴化铵(PMDB)和核酸的作用。结果表明通过共振光散射法用PMDB检测微量的DNA可靠、准确和简单。     

壳聚糖与DNA作用的共振光散射特征及微量DNA的光散射测定

通过光谱法研究壳聚糖和核酸的作用 ,结果表明通过共振光散射法用壳聚糖检测微量的 DNA方法可靠、准确且简单。