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41.
An engineered variant of the yeast Pichia pastoris was used to produce the potential malaria vaccine D1M1. This paper describes the implementation of an expanded bed adsorption chromatography step into a sequential cultivation process of the yeast in order to link protein expression, cell release, and product capture of the secreted protein in a fully automated plant. The process is operated with a semi-continuous strategy for an integrated production. Process improvements have been achieved using design of experiments. Resulting purities of product achieved up to 87% with recoveries of 51% in a single downstream operation. Furthermore, a multivariate analysis of historical batch data was used to develop a golden batch model for process monitoring. 相似文献
42.
We present a data-driven method for monitoring machine status in manufacturing processes. Audio and vibration data from precision machining are used for inference in two operating scenarios: (a) variable machine health states (anomaly detection); and (b) settings of machine operation (state estimation). Audio and vibration signals are first processed through Fast Fourier Transform and Principal Component Analysis to extract transformed and informative features. These features are then used in the training of classification and regression models for machine state monitoring. Specifically, three classifiers (K-nearest neighbors, convolutional neural networks and support vector machines) and two regressors (support vector regression and neural network regression) were explored, in terms of their accuracy in machine state prediction. It is shown that the audio and vibration signals are sufficiently rich in information about the machine that 100% state classification accuracy could be accomplished. Data fusion was also explored, showing overall superior accuracy of data-driven regression models. 相似文献
43.
This paper summarizes the basics of pulsed thermal nondestructive testing (TNDT) including theoretical solutions, data processing algorithms and practical implementation. Typical defects are discussed along with 1D analytical and multi-dimensional numerical solutions. Special emphasis is focused on defect characterization by the use of inverse solutions. A list of TNDT terms is provided. Applications of active TNDT, mainly in the aerospace industry, are discussed briefly, and some trends in the further development of this technique are described. 相似文献
44.
Qiang Xia Tao Feng Xiaowei Lou Ying Wang Yangying Sun Daodong Pan Jinxuan Cao 《International Journal of Food Science & Technology》2020,55(2):669-680
As a decisive attribute, flavour could be influenced by HP treatments through multiple physical and chemical pathways within the high pressure (HP)-assisted meat curing process. This investigation aimed to identify the major pathway influencing volatile flavour patterns of two representative vinasse-cured duck (VCD) products with HP treatments (150–300 MPa/15 min), including wet and dry types, by employing headspace fingerprinting as an untargeted approach. Results suggested that HP treatments greatly lowered moisture contents and increased Warner-Bratzler shear force and thiobarbituric acid reactive substances of the cured samples. According to multivariate models, the volatile flavour patterns of the HP-processed VCD could be clearly separated from the unprocessed samples, but the VCD pressurised at different intensities represented similar volatile fingerprinting, which was validated by e-nose analysis. The discriminant analysis (OPLS-DA) model outlined vinasse-derived ethanol, acetic acid, 3-methyl-1-butanol, 2-methyl-1-butanol, phenethyl alcohol and 2-methyl-3-octanone as the major discriminant aromas across the unpressurised and pressurised samples. 相似文献
45.
Background/purposeMeasurements of strains in critical components are often required in addition to finite element calculations when evaluating a structure.MethodsThis paper describes how standard optical fibers, bonded to the surface or embedded in a laminate, can measure strain fields along the entire length of the fiber, using the optical backscatter reflectometer.ResultsA strain field measurement can be much better compared to simulations than the more common single point measurements with strain gauges or Bragg Gratings. Changes of the strain field can be related to damage development and can be used for structural health monitoring. Practical aspects of using the fibers are also discussed.ConclusionDistributed Fiber-Optic Sensing was successfully embedded and bonded to a composite joint. Adhesive damage was identified and the strain field agreed well with FE-Analysis. 相似文献
46.
Modern refractory castables contain between 3.5 and 5?wt.-% water that is necessary for sufficient flow during emplacement and for the formation of hydrate phases, necessary for the green strength of the material. Prior to the high temperature use of this material, it must be dried very carefully to avoid explosive spalling.This paper will demonstrate that beside conventional drying of pre-shaped materials in resistance furnaces microwave radiation is an energy saving and rapid method to remove pore water as well as hydrate bond water from the castable. In comparison to resistance furnaces, the use of microwave radiation does not affect the castable properties as there are mechanical strength (MOR, CCS), open porosity and pore size distribution. This study proved microwave radiation as valuable alternative with a series of tabular alumina based low cement castables (LLC) in which the water-to-cement-ratio (wcr = 0.64, 0.75, 0.82 and 1.13) was systematically altered by changing the cement concentration at constant mixing water concentration of 4.5%. 相似文献
47.
Zhimei Zheng Taixiu Liu Qibin Liu Jing Lei Juan Fang 《International Journal of Hydrogen Energy》2021,46(38):19846-19860
Solar thermochemical hydrogen production with energy level upgraded from solar thermal to chemical energy shows great potential. By integrating mid-and-low temperature solar thermochemistry and solid oxide fuel cells, in this paper, a new distributed energy system combining power, cooling, and heating is proposed and analyzed from thermodynamic, energy and exergy viewpoints. Different from the high temperature solar thermochemistry (above 1073.15 K), the mid-and-low temperature solar thermochemistry utilizes concentrated solar thermal (473.15–573.15 K) to drive methanol decomposition reaction, reducing irreversible heat collection loss. The produced hydrogen-rich fuel is converted into power through solid oxide fuel cells and micro gas turbines successively, realizing the cascaded utilization of fuel and solar energy. Numerical simulation is conducted to investigate the system thermodynamic performances under design and off-design conditions. Promising results reveal that solar-to-hydrogen and net solar-to-electricity efficiencies reach 66.26% and 40.93%, respectively. With the solar thermochemical conversion and hydrogen-rich fuel cascade utilization, the system exergy and overall energy efficiencies reach 59.76% and 80.74%, respectively. This research may provide a pathway for efficient hydrogen-rich fuel production and power generation. 相似文献
48.
A Distributed Virtual Environment (DVE) system offers a computer-generated virtual world in which individuals located at different
places in the physical world can interact with one another. In order to achieve real-time response for a large user base,
DVE systems need to have a scalable architecture. In this paper, we present the design of a grid-enabled service oriented
framework for facilitating the construction of scalable DVE systems on computing grids. A service component called “gamelet”
is proposed, whose distinctive mark is its high mobility for supporting dynamic load sharing. We propose a gamelet migration
protocol which can ensure the transparency and efficiency of gamelet migration, and an adaptive gamelet load-balancing (AGLB)
algorithm for making gamelet redistribution decisions at runtime. The algorithm considers both the synchronization costs of
the DVE system and network latencies inherent in the grid nodes. The activities of the users and the heterogeneity of grid
resources are also considered in order to carry out load sharing more effectively. We evaluate the performance of the proposed
mechanisms through a multiplayer online game prototype implemented using the Globus toolkit. The results show that our approach
can achieve faster response times and higher throughputs than some existing approaches.
This research is supported in part by the China National Grid project (863 program) and the HKU Foundation Seed Grant 28506002. 相似文献
49.
GIS在深圳市三防指挥信息系统中的应用 总被引:1,自引:0,他引:1
深圳市三防指挥信息系统利用GIS技术,结合水利(水务)、防汛、抗旱的具体业务,建立了水情遥测数据处 理分系统、工况监测数据处理分系统、水务行业三防信息管理分系统、决策支持分系统、水源优化调度分 系统,提高了现代化管理水平。 相似文献
50.
A Lattice Approach to Image Segmentation 总被引:1,自引:0,他引:1
Jean Serra 《Journal of Mathematical Imaging and Vision》2006,24(1):83-130
After a formal definition of segmentation as the largest partition of the space according to a criterion σ and a function
f, the notion of a morphological connection is reminded. It is used as an input to a central theorem of the paper (Theorem
8), that identifies segmentation with the connections that are based on connective criteria. Just as connections, the segmentations can then
be regrouped by suprema and infima. The generality of the theorem makes it valid for functions from any space to any other
one. Two propositions make precise the AND and OR combinations of connective criteria.
The soundness of the approach is demonstrated by listing a series of segmentation techniques. One considers first the cases
when the segmentation under study does not involve initial seeds. Various modes of regularity are discussed, which all derive
from Lipschitz functions. A second category of examples involves the presence of seeds around which the partition of the space
is organized. An overall proposition shows that these examples are a matter for the central theorem. Watershed and jump connection
based segmentations illustrate this type of situation. The third and last category of examples deals with cases when the segmentation
occurs in an indirect space, such as an histogram, and is then projected back on the actual space under study.
The relationships between filtering and segmentation are then investigated. A theoretical chapter introduces and studies the
two notions of a pulse opening and of a connected operator. The conditions under which a family of pulse openings can yield a connected filter are clarified. The ability of segmentations
to generate pyramids, or hierarchies, is analyzed. A distinction is made between weak hierarchies where the partitions increase
when going up in the pyramid, and the strong hierarchies where the various levels are structured as semi-groups, and particularly
as granulometric semi-groups.
The last section is based on one example, and goes back over the controversy about “lattice” versus “functional” optimization.
The problem is now tackled via a case of colour segmentation, where the saturation serves as a cursor between luminance and
hue. The emphasis is put on the difficulty of grouping the various necessary optimizations into a single one. 相似文献