Analysis of culture fluorescence by a fiber-optic sensor inNicotiana tabacum plant cell culture

The on-line sensing of viable cell weight in plant cell culture process is applied to analysis and control of process. The fiber-optic fluorescence sensor was constructed to measure the NADH-dependent fluorescence inNicotiana tabacum plant cell culture and the analysis of fluorescence signal was done to be correlated with the viable cell weight. The structured kinetic model for cell growth was proposed to estimate the theoretical viable cell weight. The dimensional analysis was proposed for the interpretation of fluorescence signal, in which the path length, the inner filter effect and the hydrodynamic conditions were considered as the key factors on fluorescence signal. The dimensional analysis and empirical correlation of fluorescence signal to viable cell weight was applied to the interpretation of the detected fluorescence signal during cultivation. The proposed interpretation of fluorescence signal using dimensional analysis was well correlated with the viable cell weight estimated by the structured kinetic model as well as by empirical correlation.     

玻纤增强石油发酵尼龙的热稳定性研究

利用热重动力学方法研究了玻纤增强ＰＦ尼龙（即石油发酵尼龙）的热稳定性能。玻纤和偶联剂均对ＰＦ尼龙具有热稳定化作用，且硅烷Ａ１１００对ＰＦ尼龙的热稳定化作用最为显著。     

Q235钢海洋大气腐蚀暴露试验研究

采用Q235钢在海南万宁距海岸95m、25m和海洋平台3个暴露点进行了半年大气腐蚀暴露试验,同时持续监测各暴露点空气中的氯离子含量.利用视频显微镜观测样品锈层的腐蚀形貌,采用比浊法测定腐蚀产物中氯离子含量,使用FTIR光谱仪分析锈层的物相组成.结果表明,样品的朝阳面和背阳面腐蚀形貌存在较大差异,各暴露点样品腐蚀深度与各点空气中及锈层中的氯离子含量密切相关,腐蚀产物的主相为γ-FeOOH和Fe3O4,次相为α-FeOOH和δ-FeOOH.     

Thermal Analysis of Grinding

Thermal damage is one of the main limitations of the grinding process, so it is important to understand the factors which affect grinding temperatures. This paper presents an overview of analytical methods to calculate grinding temperatures and their effect on thermal damage. The general analytical approach consists of modeling the grinding zone as a heat source which moves along the workpiece surface. A critical factor for calculating grinding temperatures is the energy partition, which is the fraction of the grinding energy transported as heat to the workpiece at the grinding zone. For shallow cut grinding with conventional abrasive wheels, the energy partition is typically 60%-85%. However for creep-feed grinding with slow workspeeds and large depths of cut, the energy partition is only about 5%. Such low energy partitions are attributed to cooling by the fluid at the grinding zone. Heat conduction to the grains can also reduce the energy partition especially with CBN abrasives which have high thermal conductivity. For High Efficiency Deep Grinding (HEDG) using CBN wheels with large depths of cut and fast workspeeds, preheated material ahead of the grinding zone is removed together with the chips, thereby lowering the temperature on the finished surface. Analytical models have been developed which take all of these effects into account. Much more research is needed to better understand and quantify how grinding temperatures affect the surface integrity of the finished workpiece.     

煤质分析报告与燃烧计算

燃烧计算所需要的工作质的碳、氢、氧、氮、硫、灰分和水分7项成分的煤质分析数据,一般的煤质分析报告提不出来。本文作了某些简化的、近似的工程处理。计算出“空气干燥基”的上述7项成分数值,用以代替常用的工作基成分,通过实例计算,发现能很好地与实际情况相符合,提示这种工程处理在实用上的可行性。     

涂料用稀土催干剂中稀土金属含量分析研究

在一定条件下，以ＥＤＴＡ为络合剂，以二甲酚橙为指示剂，用络合滴定法测定了涂料用稀土催干剂中稀土金属的含量。     

Bromate Ion Analysis By Ion Chromatography

Bromate ion occurs during ozonation of bromide-containing waters. The current WHO guideline for bromate ion is 25 μg/L. Bromate analysis in drinking waters can be performed by various techniques. However, given the commonly low concentrations of bromate ion found in drinking waters, the classical methods do not fit for bromate analysis in most cases.

A specific conductimetric method with anion suppression which enhances analyte detection by lowering the eluent conductivity is described in this paper for low bromate level analysis. Three eluents have been tested in order to have the best signal to noise ratio. Some other parameters likely to interfere in bromate ion detection (nitrate and sulfate in particular) are also investigated. Taking into account the results of three interlaboratory trials between six European laboratories, a 2 μg/L detection level for bromate can be established.     

Modified Indigo Method For Gaseous And Aqueous Ozone Analyses

The indigo method developed by Bader and Hoigné for aqueous ozone analysis was modified to allow for both gaseous and aqueous ozone determination. Gas or water samples were extracted with a gas-tight syringe containing a known volume of indigo reagent. The modified procedure provided a more consistent basis for gaseous and aqueous ozone determination allowing for more accurate ozone mass balance calculations. Direct gaseous ozone UV absorbance with molar absorptivity of 3,000 M^?1cm^?1 at 258 nm was used as primary standard to determine the molar absorptivity of the indigo reagent. The molar absorptivity of indigo reagent, assuming a 1:1 stoichiometric ratio for the reaction between indigo and ozone, was determined to be 23,150 ± 80 M^?1cm^?1, or approximately 16 percent higher than that of 20,000 M^?1cm^?1 suggested by Bader and Hoigné. An independently calibrated membrane-electrode ozone monitor showed good correlation with indigo method results using the molar absorptivity value determined in this study. The apparent molar absorptivity of aqueous ozone at the wavelength of 258 nm measured by the modified indigo method increased from 2,400 to 3,600 M^?1cm^?1 in the investigated ozone concentration range of 0.4 to 11.0 mg/L. This variation might have been caused by the inherent interference of unidentified ozone byproducts, which presence was supported with scanning spectra in the wavelength range of 200 to 300 nm.     

中厚壁钢管三辊轧制内裂原因分析

在三辊轧管机上采用不同的变形量进行轧制对比实验,分析了三辊轧制过程中产生的切向、轴向剪切应力及塑性弯曲变形时产生的切向、轴向拉应力对内裂的影响。实验结果表明:在其他工艺条件相同时,三辊轧制的变形量超过一定值时,钢管内表面金属破裂。针对中厚壁钢管内表面纵裂纹形成的主要原因,提出了解决措施。     

管线用钢氢致裂纹(HIC)影响因素分析

研究了管线用钢的化学成分和金相组织对钢的氢致裂纹(HIC)性能的影响。研究认为,带状组织和马氏体含量对HIC性能有很大影响,S含量及Cep对HIC有不同程度影响。含有少量Cu、Ni的钢更适合在酸性环境下使用。