Regulatory Light Chains in Cardiac Development and Disease

The role of regulatory light chains (RLCs) in cardiac muscle function has been elucidated progressively over the past decade. The RLCs are among the earliest expressed markers during cardiogenesis and persist through adulthood. Failing hearts have shown reduced RLC phosphorylation levels and that restoring baseline levels of RLC phosphorylation is necessary for generating optimal force of muscle contraction. The signalling mechanisms triggering changes in RLC phosphorylation levels during disease progression remain elusive. Uncovering this information may provide insights for better management of heart failure patients. Given the cardiac chamber-specific expression of RLC isoforms, ventricular RLCs have facilitated the identification of mature ventricular cardiomyocytes, opening up possibilities of regenerative medicine. This review consolidates the standing of RLCs in cardiac development and disease and highlights knowledge gaps and potential therapeutic advancements in targeting RLCs.     

In Vitro Characterization of Human CD24hiCD38hi Regulatory B Cells Shows CD9 Is Not a Stable Breg Cell Marker

Regulatory B (Breg) cells are endowed with immune suppressive functions. Various human and murine Breg subtypes have been reported. While interleukin (IL)-10 intracellular staining remains the most reliable way to identify Breg cells, this technique hinders further essential functional studies. Recent findings suggest that CD9 is an effective surface marker of murine IL-10 competent Breg cells. However, the stability of CD9 and its relevance as a unique marker for human Breg cells, which have been widely characterized as CD24^hiCD38^hi, have not been investigated. Here, we demonstrate that CD9 expression is sensitive to in vitro B cell stimulations. CD9 expression could either be re-expressed or downregulated in purified CD9-negative B cells and CD9-positive B cells, respectively. We found no significant differences in the Breg differentiation capacity of the CD9-negative and CD9-positive B cells. Furthermore, CD9-positive B cells co-express CD40 and CD86, suggesting their nature as B cell activation or co-stimulatory molecules, rather than regulatory ones. Therefore, we report the relatively unstable CD9 as a distinct surface molecule, indicating the need for further research for a more reliable marker to purify human Breg cells.     

Clinical Significance of Tumor Infiltrating Lymphocytes in Association with Hormone Receptor Expression Patterns in Epithelial Ovarian Cancer

Hormone receptor expression patterns often correlate with infiltration of specific lymphocytes in tumors. Specifically, the presence of specific tumor-infiltrating lymphocytes (TILs) with particular hormone receptor expression is reportedly associated with breast cancer, however, this has not been revealed in epithelial ovarian cancer (EOC). Therefore, we investigated the association between hormone receptor expression and TILs in EOC. Here we found that ERα, AR, and GR expression increased in EOC, while PR was significantly reduced and ERβ expression showed a reduced trend compared to normal epithelium. Cluster analysis indicated poor disease-free survival (DFS) in AR+/GR+/PR+ subgroup (triple dominant group); while the Cox proportional-hazards model highlighted the triple dominant group as an independent prognostic factor for DFS. In addition, significant upregulation of FoxP3+ TILs, PD-1, and PD-L1 was observed in the triple dominant group compared to other groups. NanoString analyses further suggested that tumor necrosis factor (TNF) and/or NF-κB signaling pathways were activated with significant upregulation of RELA, MAP3K5, TNFAIP3, BCL2L1, RIPK1, TRAF2, PARP1, and AKT1 in the triple dominant EOC group. The triple dominant subgroup correlates with poor prognosis in EOC. Moreover, the TNF and/or NF-κB signaling pathways may be responsible for hormone-mediated inhibition of the immune microenvironment.     

Facile Fabrication of Poly(vinyl alcohol)/Polyquaternium-10 (PVA/PQ-10) Anion Exchange Membrane with Semi-Interpenetrating Network

Anion exchange membranes (AEMs) are one of the core components of AEM fuel cells. A series of poly(vinyl alcohol)/polyquaternium-10 (PVA/PQ-10) AEMs with semi-interpenetrating networks (s-IPNs) are prepared by a simple solution-casting method using glutaraldehyde (GA) as a cross-linking agent. Subsequently, the prepared PVA/PQ-10 cross-linked membranes are characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, mechanical analysis, water uptake and swelling ratio tests, ion exchange capacity (IEC) tests, ionic conductivity measurements, and oxidative/alkaline stability tests. The effects of the mass ratio of PVA and PQ-10 and the amount of cross-linking agent GA on the performance of the PVA/PQ-10 cross-linked membranes are systematically explored. The results show that the cross-linked PVA/PQ-10 AEMs have high IEC and low water uptake and swelling ratio, and its maximum ionic conductivity can reach 79.37 mS cm^–1 at 80 °C. In addition, the PVA/PQ-10 cross-linked membrane has good oxidative and alkaline stability under optimal preparation conditions. These results may provide valuable insights toward more effective scheme designs and new, simple preparation methods for AEMs with s-IPN structures.     

基于神经网络的助力搬运装置自适应控制方法研究

为提高助力搬运装置在建筑工地等复杂路况下的适用性,通过对国内外助力系统及其控制方法发展现状的调查和分析,提出一种智能助力控制系统。首先建立控制模型,在智能控制的研究中发现BP神经网络能够对输入信息进行识别并分类处理,且计算较简单,容易实现,但BP神经网络隐含层节点数难以确定、易陷入局部最小值,因此,采用遗传算法对BP神经网络进行优化(GA-BP),并设计具体实现方法。采集平坦、斜坡两种路况的数据,通过人工赋予理想输出数据,将其一部分作为训练数据,对GA-BP神经网络及BP神经网络进行对比训练,另一部分作为验证数据,检验两种网络的训练结果。使用MATLAB进行数据仿真分析,验证GA-BP神经网络控制方法的可行性。试验结果表明:这种控制方法能够有效地解决助力搬运装置在复杂路况下的自适应控制问题。     

Intelligent temperature control system of quench furnace

1　INTRODUCTIONQuenchfurnaceisakindofkeyequipmentusedtoheattreatlargecomplexcomponentsofaircraftsorrockets ,withheightof 2 4m ,containingtwoheatingroomsandaworkroom ,withsteelboardssetbetweentherooms .Bothheatingroomsareseparatedintosixsections ,eachofwhichcontainsaheatingresistancecontrolledbyapoweradjuster .Workpiecesareputinworkroom .Acentrifugalelectromotorisusedtoblowhotairintoworkroomfromheatingroom .Thede mandofalloyintemperature risingrateandstableve racityatfixedtemperaturepointi…     

智能控制在气动比例位置系统中的应用

本文对由比例阀和无杆汽缸组成的气动位置系统在线性化的基础上进行了数学建模，对模型引入白噪声干扰，它通过噪声模型作用与对象输出端，并利用Hammerstein模型模拟了对象的非线性本质，通过计算机仿真研究了基于遗传算法寻优的神经模糊网络控制的应用效果。与实际结果进行比较，仿真效果良好。     

工艺参数对微区沉淀法制备纳米MgAl2O4粉体的影响

采用高分子网络微区沉淀法制备纳米镁铝尖晶石（MgAl2O4）粉体，并采用TGA／DTA、XRD和TEM等分析测试技术对前驱体的热分解和所制得粉体的物相组成、形貌及颗粒尺寸进行表征，结果表明所制得的MgAl2O4粉体颗粒粒径小，结晶良好，成分单一且粒径大小均匀。而实验过程中工艺参数的改变会对生成粉体的粒径和形貌产生一定的影响，进而即研究了各种工艺参数的改变对该方法制备纳米MgAl2O4粉体的影响。     

免疫遗传算法在烧结矿配料优化中的应用

运用免疫遗传算法和MATLAB语言 ,编制了钢铁烧结矿配料优化计算仿真程序 ,计算机仿真结果表明符合实际生产工艺要求 ,可直接应用于生产 ,提高各种烧结矿的综合利用率 ,降低成本 1 0 32 %。从而证明了免疫遗传算法是一种切实有效的优化方法 ,该算法为钢铁烧结矿配料问题的优化求解提供了新的思路和方法。     

基于遗传算法的图像恢复研究

通过对遗传算法和图像恢复理论的分析，提出了基于遗传算法的图像恢复方式。该方式具有优化、收敛性快等优点。