从实例解读屈米的空间三类型

空间的真实性一直是建筑师关注和探讨较多的问题.美国著名建筑大师伯纳德·屈米则从真实空间、事件和功能以及三者之间的关系出发,提出按照与周围环境和文脉(context)关系,空间可有三种类型.该文即是从我们生活的城市中寻找这三种空间类型,来解读屈米的真实空间.     

Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology

We describe a homogeneous competitive immunoassay for a phosphorylated protein antigen. The assay takes advantage of the enhanced fluorescence resonance energy transfer (FRET) technology, which has a unique characteristic that the FRET signal is increased by the specific interaction of two fluorolabeled leucine zippers. We chose extracellular signal-regulated kinase (ERK) as a model antigen and constructed two molecular probes in which either anti-phosphorylation site antibody or the antigen peptide was chemically conjugated to the enhanced FRET probes. While these molecular probes indicated sufficient FRET signal without antigen, they displayed a significant change in the fluorescent spectrum by mixing with phosphorylated antigens. With this competitive enhanced FRET immunoassay, a phosphorylated ERK concentration within the range from 15 nM to 250 nM could be determined. Because the assay is very simple, it would be applied to not only in vitro assay but also in vivo detection of protein phosphorylation.     

Effects of ripening on microstructure and texture of “Ameixa d’Elvas” candied plums

The plums used to produce a traditional candied product, “Ameixa d’Elvas”, were obtained from two orchards, Vila Viçosa (VV) and Cano (CA). These orchards were selected because the fruits were behaving differently: (1) The day of harvesting for candying, established by the total soluble solids and titratable acidity, was one week earlier in VV; (2) VV yielded candied plums with good texture properties whereas CA gave poor processed fruits. In order to understand the origin of these differences, fruits from both orchards were harvested on the day established as the harvesting day for VV (day 1) and for CA (day 8). Comparable texture properties were obtained in firmness, rigidity and deformation work between the VV fresh plums harvested on day 1 and the CA plums harvested on day 8 but these were lower in CA when the flesh was analysed separately, in accordance with the activities of pectin methyl esterase (PME), polygalacturonase (PG) and cellulase (Cel). The increase of the intercellular area of parenchyma cells and the decrease in cell area caused by boiling, which resulted in a pronounced loss of textural properties, were partially recovered after the immersion of the fruits in sucrose syrup. The CA plums harvested on day 8 had a more pronounced degradation with boiling and lower recovery of cell shape, size and textural characteristics than had those of VV harvested on day 1. Upon candying, similar properties were observed for the fruits harvested on the same day: good candied products were observed for VV and CA fruits harvested on day 1 and poor candied products were observed for VV and CA fruits harvested on day 8. This work shows that the characteristics of the flesh of the fresh fruits are key parameters in defining the textural properties of the candied plum. The establishment of the harvesting moment for candying should take into account the changes that occur in the flesh of the plums during ripening.     

一种融合Gabor滤波与3D/2D卷积的高光谱图像分类算法

为了减轻卷积神经网络模型对训练样本的依赖性和提高高光谱图像的分类性能,本文提出了一种融合Gabor滤波与3D/2D卷积的高光谱图像分类算法.首先,三维Gabor滤波器用于处理原始高光谱数据以生成空谱隧道信息;其次,利用三维卷积操作提取生成的空谱隧道信息的深层特征;然后,再利用二维卷积进一步提取图像的空间信息;最后,通过...     

变桩长与变桩距技术在处理桥头软基中的应用

在软土地基上修建高等级公路时,“桥头跳车问题”是一个技术难题,利用变桩长与变桩距的预制管桩复合地基技术,在桥头软基上设置过渡段,可使堆载排水固结法处理的一般路段与桥头之间的工后沉降平稳过渡,缓解了此两种工法处理路段的沉降差异,并能减小桥头段的纵坡率,利用该技术在杭州湾跨海大桥南岸接线段高速公路试验段的桥头软基处理中效果理想。     

双层桥面大跨钢管拱面内极限承载力分析

通过建立空间复合梁单元,将钢管拱根据不同的材料划分为纵向的纤维,每根纤维在纵向分段,纵向的分段通过取若干积分点来实现,采用这样的方法可以更加全面地追踪结构极限承载力的发展过程,并把这种方法用于分析大跨径钢管混凝土拱桥,取得了较好的效果。     

家庭服务机器人智能空间技术研究与进展

智能空间技术是一个刚刚兴起的前沿课题.应用于家庭服务机器人的智能空间技术,就是把感知器件、执行器件分布地安装在空间中相应的位置,实现对空间内部人和物的全面感知,从而通过执行器实现相应的服务任务;同时能够为机器人提供更加完备的环境信息,使得机器人本身可以降低对于本体携带感知器件的要求,能够“轻装上阵”,在未知或半未知的动态环境中实现更加快捷、准确、稳定的家庭服务工作.系统总结了智能空间技术的发展现状,并详细介绍了应用于家庭服务机器人的智能空间的构成、特点及其主要研究内容,对其所涉及的相关技术进行了介绍,最后给出了家庭服务机器人智能空间技术研究的新方向.     

创造符合寒地特征的城市公共空间以哈尔滨为例

文章从寒地的气候特征出发,分析了寒地城市在公共空间创造中应当注意的原则与方法,结合哈尔滨的城市空间创造实践,探讨如何创造符合寒地地域特征、体现城市历史文化内涵的城市公共空间。     

绿锈的研究进展

综述了绿锈的结构组成等特性,介绍了绿锈的合成方法、稳定性影响因素及应用,并对其发展作了展望。     

模糊控制技术在大空间二次回风空调系统中的应用

介绍了模糊控制技术在一交响乐厅二次回风空调系统中的应用。针对该工程实际情况提出了完整的控制策略,设计了新风阀、二次回风阀和冷水阀的模糊控制器,以保证室内温湿度和空气质量达到设计要求。以建筑物集成管理系统为操作平台,在中央管理计算机上实现了各设定值的优化,由现场DDC完成闭环控制。