计算机语言类课程实践环节教学改革探讨

针对计算机语言课程教学中重理论、轻实践,学生编程能力较弱的状况,通过对实践环节存在的问题进行分析,从管理监督机制、教材建设、兴趣引导、网络资源建设、教学方法和考核等方面提出了改革措施,激发了学生编程的兴趣与主动性,提高了编程能力,收到了预期的效果。     

人白细胞介素-24基因的克隆、表达和纯化

目的对人白细胞介素-24(hIL-24)进行克隆、表达和纯化。方法分离人外周血单个核细胞,ConA刺激培养,提取细胞总RNA,应用RT-PCR技术从外周血单个核细胞中扩增hIL-24成熟肽编码区,定向克隆至融合表达载体pGEX-4T-1,重组载体pGEX-4T-1/hIL-24经DNA测序鉴定后,转化E.coliBL21(DE3),IPTG诱导表达,阴离子交换层析纯化融合蛋白,SDS-PAGE和Westernblot鉴定。结果所得hIL-24基因经序列分析,与GenBank公布一致。所构建融合表达载体pGEX-4T-1/hIL-24测序正确,转化BL21(DE3)后,37℃诱导表达相对分子质量约为44000的融合蛋白,主要以包涵体形式存在,占菌体总蛋白的30.63%。经纯化后,纯度达85%以上。Westernblot检测能与兔抗人IL-24的多克隆抗血清发生结合反应。结论已成功构建了hIL-24的融合表达载体pGEX-4T-1/hIL-24,并在大肠杆菌中高效表达,纯化后获得了高纯度的融合蛋白,为hIL-24的功能及活性研究奠定基础。     

基于信息物理融合的骨折复位机器人系统构建

针对骨折治疗中传统复位方法（手法复位、牵引复位、手术复位）存在的二次损伤、肌能失调、患肢僵硬、破坏血运及术后矫正动态性能差等问题,提出了一种人-机-物融合的信息物理系统（CPHS）用于指导机器人的复位动作。首先,从CPHS的数字孪生、信息感知、系统集成、手术流程、模拟复位等方面论证了并联机器人信息物理系统的构成,将机器人的高定位精度及可重复性与微创方法有效结合,从而指导医生完成仿真规划及术中监控等一系列操作;其次,根据临床骨折复位过程,在机器人操作下对5组不同骨折姿态的模拟骨折病例进行了复位实验;最后,计算出每组实验复位后的移位残余及角度误差,并与传统复位方法的相应数据进行对比。实验结果表明,CPHS骨折复位机器人相较于传统复位方法,在骨折复位过程和患者术后康复上均有着明显的优势。     

Cognitive work analysis to comprehend operations and organizations in the mining industry

Complex industrial systems, including mining, have a prominent challenge in understanding the interrelationship among the cognitive processes, working environment and available equipment. The concept of cognitive work analysis(CWA) transcends the traditional analytic methods of evaluating human tasks solely based on perceptual and physical traits, and rather implements the notions of behavioral and cognitive awareness indispensable for the intricacy of modern technology. In the last few decades, academic and industrial settings employ this type of analysis to set a suitable standard for a system's safety feasibility, and as a result reduce human-based errors. This research paper analyzes current CWA methods and proposes a five-level quantification model portraying the overall cognitive quality of a mining operation.     

Scanning electron microscopy of nerve fibers in human fetal cochlea

The cochleas of four human fetuses ranging 22–25 weeks gestation were studied by scanning electron microscopy (SEM) for the purpose of obtaining a better understanding of the nerve fiber arrangement in the human ear. After critical point drying, the specimens were dissected and the floor of the tunnel of Corti and the outer wall of Nuel's space were exposed for observation. Upper cochlear turns, especially the apical turn, seemed to be still immature. Observed nerve fibers were classified into three types:

• 1 Spiral fibers: Fibers traveling basalward and following the shape of the cochlea were found in both the tunnel of Corti and Nuel's space and believed to be the afferent nerves responsible for innervating the outer hair cells
• 2 Radial fibers: radiating outward from the osseous spiral lamina—one such radial fiber transversing high in the tunnel space (supposedly the efferent nerve servicing the outer hair cells), and another sort of radial fiber (found crossing the tunnel floor), the nature of which was uncertain.
• 3 Irregular fibers: Consisting of thin, randomly running fibers within the cochlea. The destination of these fibers was not determined, but possibly they represent transitory nerve branchings of afferent or more probably efferent nerves, which would later regress during maturation.

     

火电厂工程项目管理创新模式及基建实践探讨

由于火电厂建设具备"投资大、专业性强、工期长、占地面积大、交叉作业多、施工工序复杂、危险系数大"等特点,基建管理难度大,为此强化项目管理创新显得尤为重要。"六精"管理模式是华电六安电厂在基建期间为实现"精品创优"目标不断总结、提炼而成的项目管理创新成果,并在实践中取得了良好的综合效益,具有实践效果佳、操作性好、可参考性强等特点。     

基于B/S模式的人力资源管理信息系统设计——以某高职院校为例

针对某高职院校人力资源管理信息系统现状,提出了基于B/S结构的系统架构设计和实施计划;分析了使用VS.NET和SQL Server的系统设计及实现方法。     

Assessing the effects of gamification in the classroom: A longitudinal study on intrinsic motivation,social comparison,satisfaction, effort,and academic performance

Gamification, the application of game elements to non-game settings, continues to grow in popularity as a method to increase student engagement in the classroom. We tested students across two courses, measuring their motivation, social comparison, effort, satisfaction, learner empowerment, and academic performance at four points during a 16-week semester. One course received a gamified curriculum, featuring a leaderboard and badges, whereas the other course received the same curriculum without the gamified elements. Our results found that students in the gamified course showed less motivation, satisfaction, and empowerment over time than those in the non-gamified class. The effect of course type on students' final exam scores was mediated by students' levels of intrinsic motivation, with students in the gamified course showing less motivation and lower final exam scores than the non-gamified class. This suggests that some care should be taken when applying certain gamification mechanics to educational settings.     

Empathy and Modern Technology: A Neuroergonomics Perspective

The primary purpose of this paper is to discuss the role of empathy in the design of advanced systems in manufacturing and service industries in order to ensure suitable working conditions for employees from the social and technological point of view. The origins and components of empathy are briefly reviewed. The neural underpinnings of three components of empathy, including cognitive, emotional, and behavioral aspects, are considered in the context of human–human and human–machine interactions, as well as design of working environments. Finally, the potential advantages of applying empathy‐related knowledge to the design and development of human‐centered technology are discussed.     

Maintenance Scheduling Optimization in a Multiple Production Line Considering Human Error

An analytical multiobjective maintenance planning model that maximizes reliability while minimizing cost and human error is proposed. In order to incorporate human error, the model minimizes the maximum human error over the planning horizon. Human Error Assessment and Reduction Technique (HEART) is used to quantify the human error. Maintenance activities include adjustment and replacement activities, in which each of them consumes a certain amount of human resource, spare parts, and budget and brings about a specified level of reliability and human error. Economic dependence is also considered, in which grouping maintenance activities reduces total cost. However, this may increase human error probability due to operator fatigue or time pressure. The main purpose is to investigate the relationship between human factors and maintenance activities to find the preferred maintenance plan. A multiple production line is considered as a case study. A sensitivity analysis is performed, and the effects of grouping and human factors on the preferred maintenance plan are discussed. It is shown how human proficiency may affect reliability and cost.