Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics

Glutamine synthetase (GS) is the key enzyme involved in the assimilation of ammonia derived either from nitrate reduction, N(2) fixation, photorespiration or asparagine breakdown. A small gene family is encoding for different cytosolic (GS1) or plastidic (GS2) isoforms in legumes. We summarize here the recent advances carried out concerning the quaternary structure of GS, as well as the functional relationship existing between GS2 and processes such as nodulation, photorespiration and water stress, in this latter case by means of proline production. Functional genomic analysis using GS2-minus mutant reveals the key role of GS2 in the metabolic control of the plants and, more particularly, in carbon metabolism.     

荷花的繁殖、栽培管理与景观应用

文章介绍了荷花的基本情况、形态特征和生态习性.介绍了荷花繁殖最常用的两种方法:播种繁殖和分藕繁殖.探讨了荷花的栽培管理技术,以及荷花在园林景观方面的应用.     

莲花河畔景苑7#楼倒塌后地下车库基坑设计

上海莲花河畔景苑7#住宅楼的倒塌,引起了社会各界的关注。事故发生后,市抢险指挥部和闵行区政府分别指定上海现代设计集团华东建筑设计研究院有限公司和上海建工集团第七建筑有限公司承担后续工程的设计和施工。后续地下车库基坑周边紧邻多幢住宅楼,原车库基坑的围护结构因开挖深度增加和变形控制要求的提高无法利用,成为后续基坑工程实施的障碍物,另外工程工期紧迫,这些因素成为后续基坑工程设计和施工的难点。综合现场环境、工期和施工等条件,最终确定采用型钢水泥土搅拌墙结合一道钢支撑的板式支护结构方案。基坑实施监测表明,采用的支护方案为现场施工的开展和加快工期创造了良好的条件,确保了周边住宅楼的安全,为后续工程的顺利开展创造了良好的条件。     

Lotus Domino系统Web图形化报表的实现

针对Lotus Domino Web应用报表功能的不足,探讨了XPage下利用Dojo和JSON产生图形化报表的原理和实现,丰富了Lotus Domino Web应用系统数据报表的呈现方式,使企业信息化平台中产生的数据更加形象和直观。     

荷花品种资源的新发现

为了发掘我国荷花品种资源，在云南、广东一带进行了考察，先后发现7个独特的品种或变型。对这新发现的7个品种与同类品种的花形、花色、花径、花态等做了比较分析，认为它们是由野生种演进而来，具有难得的优良性状。     

Hyperhydrophilic rough surfaces and imaginary contact angles

The complete wetting of rough surfaces is only poorly understood, since the underlying phenomena can neither be described by the Cassie‐Baxter nor the Wenzel equation. An experimental accessiblility by the sessile drop method is also very limited. The term “superhydrophilicity” was an attempt to understand the wetting of rough surfaces, but a clear definition is still forthcoming, mainly because non‐superhydrophilic surfaces can also display a contact angle of zero. Since the Wilhelmy balance is based on force measurements, it offers a technology for obtaining signals during the whole wetting process. We have obtained evidence that additional forces occur during the complete wetting of rough surfaces and that mathematically contact angles for a hydrophilicity beyond the contact angle of zero can be defined by imaginary numbers. A hydrophilized TPS‐surface obtained by chemical wettability switching from a superhydrophobic surface has been previously characterized by dynamic imaginary contact angles of 20i°–21i° and near‐zero hysteresis. Here an extremely high wetting rate is demonstrated reaching a virtual imaginary contact angle of Θ_V,Adv > 3.5i° in less than 210 ms. For a rough surface displaying imaginary contact angles and extremely high wetting rates we suggest the term hyperhydrophilicity. Although, as will be shown, the physical basis of imaginary contact angles is still unclear, they significantly expand our methodology, the range of wettability measurements and the tools for analyzing rough hydrophilic surfaces. They may also form the basis for a new generation of rationally constructed medicinal surfaces.     

基于Lotus的矿山办公自动化系统

根据矿山企业信息量大、信息流复杂的特点 ,提出以当今最先进的群件产品Lo tusDomino Notes作为矿山办公自动化系统开发工具 ,实现跨平台操作以及与Internet的无缝链接。在构建资源共享、负载均衡、实时更新以及安全可靠的网络规划基础上 ,采用基于B S结构与C S结构相结合的系统模式 ,将管理信息系统与办公系统有机集成 ,形成一个开放、安全和高效的企业办公自动化系统。     

The in vitro antioxidant activity of lotus germ oil from supercritical fluid carbon dioxide extraction

The in vitro antioxidant activity of lotus germ oil extracted by supercritical fluid extraction (SFE) has been investigated. The distinctly high total phenolic compounds content and tocopherol content in lotus germ oil composition were found to be 9.06 ± 0.11% and 485.1 ± 50 mg/100 g, respectively. The lotus germ oil exhibited a dose-dependent inhibitory effect on the hydroxyl free radical and superoxide anion free radical. However, the scavenging effects on the superoxide anion free radical were deceased when the extract concentration was greater than 70 mg/mL. Lotus germ oil showed substantial antioxidant activity in the mice liver and kidney tissues homogenates in a dose-dependent manner. The auto-haemolysis of mice red blood cells was also blocked by lotus germ oil in a dose-dependent manner. Lotus germ oil showed a higher antioxidant activity in the lard system. The high content of phenolic compounds and tocopherol in the lotus germ oil could partially account for the antioxidant activity. These results suggest the lotus germ oil can be used as healthcare oil to develop.     

Evaluation of the anti-inflammatory efficacy of Lotus corniculatus

The anti-inflammatory effects of the crude extract (CE) of Lotus corniculatus var. São Gabriel and its derived hexane (HEX), ethyl acetate (AcOEt), n-butanol (BuOH) and aqueous (Aq) fractions and isolated compounds kaempferitrin, oleanolic acid and β-sitosterol, in a mouse model of pleurisy induced by carrageenan were investigated. Swiss mice were used in the in vivo experiments. The crude extract of L. corniculatus and its derived fractions, and also its isolated compounds, inhibited leukocytes, exudation, and myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, as well as nitrite/nitrate concentration and interleukin-1 beta (IL-1β) level (p < 0.05). L. corniculatus showed important anti-inflammatory activity by inhibition not only of leukocytes and/or exudation, but also of pro-inflammatory enzymes and mediators such as MPO, ADA, and IL-1β. The constituent’s kaempferitrin, oleanolic acid and β-sitosterol may well account for it.     

Effects of chitosan-based coating and modified atmosphere packaging (MAP) on browning and shelf life of fresh-cut lotus root (Nelumbo nucifera Gaerth)

The present study investigated the effect of chitosan coating containing antibrowning agents and modified atmosphere packaging (MAP) on the browning and shelf life of fresh-cut lotus root stored at 4 °C for 10 days. The atmosphere in the packages was evaluated for O₂ and CO₂ concentrations. The browning on the surface of lotus root slices was prevented by chitosan-based coating and MAP treatment. L* values of coated + MAP and control samples were 68.8 and 48.9 after 8 days storage, respectively. For a^? and b^? values, samples of coated + MAP treatment had the slowest increasing trends among all the treatments. At the end of storage, the coated + MAP samples exhibited the lowest polyphenol oxidase (PPO) activity and malondialdehyde (MDA) content. Its highest overall visual quality (OVQ) scores (> 7) demonstrated that chitosan-based coating + MAP treatment could provide a better inhibitory effect on the browning and extend the shelf life of fresh-cut lotus root.Industrial relevance: Fresh-cut vegetables have drawn the attention of industry as a novel lightly processed product. Both edible coating and MAP treatment cause changes in atmosphere composition and respiration rate of lotus root slices. This combined treatment could be used to control the browning and improve the storage life of this fresh-cut vegetable. This information could be useful for the development of novel application to edible coating and MAP design for lightly processed lotus root.