Supercritical CO2 extraction of oil, carotenoids, squalene and sterols from lotus (Nelumbo nucifera Gaertn) bee pollen

The high-quality oil, abundant in carotenoids, squalene and sterols (mainly consisting of campesterol, stigmasterol, β-sitosterol and β-amyrin), was extracted by supercritical CO₂ from lotus bee pollen for its potential nutraceutical use. The effects of extraction pressure and temperature on the yields and the compositions of the extracts were investigated by using a two-factor central composite rotatable design experiment. ANOVA for response surface model demonstrated that the data were adequately fitted into four polynomial models. The yields of the oil, carotenoids, squalene and sterols were significantly influenced by the experimental variables. It was predicted that maximum oil yield obtained at the extraction pressure of 38.2 MPa and temperature of 49.7 °C contained the maximum amount of carotenoids, squalene and sterols. GC-FID analysis of the fatty acid composition of lotus bee pollen oil showed that polyunsaturated fatty acids accounted for approximately 22% of the total fatty acids.     

Lotus Domino与基TRDBMS的应用系统的实时连接研究与实现

目前使用Lotus Domino作为服务端平台的OA系统越来越广泛的应用于各个企事业单位,它已经成为办公企业系统中较成熟的一种办公电子协作平台了.当然这与它结构上的特殊的安全性是有很大的关系的.在它的整个结构中,从底层到顶层在网络信道,服务器,数据库,文档,表单视图,区段,域等方面均有各层的安全控制机制,而且在加密的技术上还采用了非对称的加密方式来对用户进行身份的验证,实现防止网络侦听,以及信道传输的安全.总之,Lotus Domino这一服务平台在近些年来的办公化自动系统中的应用是相对稳定,配置管理比较灵活的.已经是一个相当成熟的产品了,但是它作为企业协作办公的一个平台,也存在一定的局限性.OA办公系统要想有效的实现信息资源的共享,领导监控管理.使得企业管理完善自动化,就必须在效率上有所突破,而Lotus Domino做为OA办公系统的服务平台,它自身采用的则是文本型数据库,这就大大的限制与目前众多以关系犁数据库为开发平台的应用软件进行交互的机能,所以实现从Lotus Domino数据库向关系型数据库的数据迁移,使Lotus Domino与RDBMS的应用系统进行实时连接的研究,将会使得OA系统能与外部的应用系统实时交互,使之更加符合现代企事业单位的需求,使得企事业单位在管理应用上更加完善化,一体化,高效化.     

Unified design: collaborative working at Arup Associates

Global telecommunication networks and the emergence of a new digital tool base have informed the reshaping of design practice worldwide. As discussed in detail in the next issue of AD, Collective Intelligence in Design (no 5, vol 76, 2006), ever-evolving groups of designers, engineers, material scientists, manufacturers, contractors and even financiers are now working together across time zones and organisations. Though these current shifts have certainly facilitated a greater fluidity of exchange between disciplines, working across the professional setting is not a wholly new phenomenon. For over four decades, London-based Arup Associates have collaborated in design as a team of architects and engineers, sitting within the greater corporate framework of the Arup Group. Declan O'Carroll, a principal at Arup Associates, tells Helen Castle how Arup Associates are now launching the Unified Design Unit that is set to recast Ove Arup's ethos of totality in design for the 21st century. Copyright © 2006 John Wiley & Sons, Ltd.     

荷叶提取物抗氧化活性的研究

本文研究了荷叶在食用大豆油中的抗氧化活性 ,结果表明甲醇的荷叶提取物有很强的抗氧化活性 ,其抗氧化性与BHA相当 ,略高于α—生育酚 ,可抑制约 74%的亚麻酸发生氧化反应。用薄层层析将该提取物分成 6部分 ,其中Rf=0 .88和Rf=0 .80的两部分具强抗氧化活性 ,分别可抑制 79.6 %和 71.1%的亚麻酸发生过氧化反应。     

莲藕、茅根、菊花复合清凉饮料的研制

研究了莲藕、茅根、菊花复合饮料的制作过程,并对生产工艺技术进行了探讨。     

莲房原花青素体外抗肿瘤作用研究

目的:研究莲房原花青素(LSPC)的相对分子量分布及体外抗肿瘤作用。方法:用ESI-MS分析LSPC,MTT法检测人肺癌LETP-2、胃腺癌SGC-7901、黑色素瘤A3753种细胞增殖,采用平板集落实验测定3种癌细胞集落形成。结果:LSPC的分子量范围为290.1～1154.3,是以二聚体为主的低聚原花青素;一定浓度的LSPC对3种瘤细胞均有抑制作用,当LSPC浓度为100μg/mL作用72h时对3种瘤细胞生长抑制率分别为62.6%、49.8%、77.0%;集落形成抑制率分别为77.0%、43.2%、95.3%。结论:LSPC体外对LETP-2、SGC-7901、A3753种瘤细胞有显著的抑制作用。     

Roles of AGD2a in Plant Development and Microbial Interactions of Lotus japonicus

Arabidopsis AGD2 (Aberrant Growth and Death2) and its close homolog ALD1 (AGD2-like defense response protein 1) have divergent roles in plant defense. We previously reported that modulation of salicylic acid (SA) contents by ALD1 affects numbers of nodules produced by Lotus japonicus, but AGD2′s role in leguminous plants remains unclear. A combination of enzymatic analysis and biological characterization of genetic materials was used to study the function of AGD2 (LjAGD2a and LjAGD2b) in L. japonicus. Both LjAGD2a and LjAGD2b could complement dapD and dapE mutants of Escherichia coli and had aminotransferase activity in vitro. ljagd2 plants, with insertional mutations of LjAGD2, had delayed flowering times and reduced seed weights. In contrast, overexpression of LjAGD2a in L. japonicus induced early flowering, with increases in seed and flower sizes, but reductions in pollen fertility and seed setting rates. Additionally, ljagd2a mutation resulted in increased expression of nodulin genes and corresponding increases in infection threads and nodule numbers following inoculation with Rhizobium. Changes in expression of LjAGD2a in L. japonicus also affected endogenous SA contents and hence resistance to pathogens. Our results indicate that LjAGD2a functions as an LL-DAP aminotransferase and plays important roles in plant development. Moreover, LjAGD2a activates defense signaling via the Lys synthesis pathway, thereby participating in legume–microbe interaction.     

Lotus Domino系统构筑安全电子商务网站

本文论述了中小企业自建安全电子商务网站的Lotus Domino群件解决方案,提出了适应不同需求层次的安全性策略,并阐述了具体实施过程的相关问题。     

基于Lotus平台的OA文档发布技术研究

Lotus Notes是开发办公自动化软件的重要技术支撑平台,是集电子邮件、通讯软件、数据库软件、文件管理软件、工作流软件于一体的群件支持系统.在介绍了Lotus Notes的特点并对文档信息的核心-Notes ID进行了系统剖析的基础上,实现了文档发布和文档归类功能模块的设计与开发.     

基于Domino的大客户管理系统Web方式改造实践

随着Internet技术的发展,原有的基于c/s结构的电信大客户管理系统已不能满足用户的需要。因此，对原有系统进行web方式改造成为必要。论述了系统改造的总体设计思想，对Dornino Web服务器的工作原理度配置方法进行了简要的介绍，给了系统改造时存在的部分问题及解决方式．最后给出了系统的部分实现代码。