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161.
以转基因水稻中最常用的CaMV35S启动子、NOS终止子、Cry1Ab/Ac基因、HPT基因及SPS水稻内标基因为研究对象,利用5 种不同的荧光信号(FAM、HEX、Taxas Red、Cy5、Cy5.5)进行多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)检测方法的研究。通过引物组合筛选、反应体系优化、特异性测试、灵敏度测试、适用性测试等一系列实验,建立了5 重real-time PCR方法,灵敏度可达0.032%。此方法具有灵敏度高、结果准确、通量大等优点,可实现水稻中转基因成分的快速、高效检测。 相似文献
162.
A flower‐shaped ultra‐wideband fractal antenna is presented. It comprises a fourth iterative flower‐shaped radiator, asymmetrical stub‐loaded feeding line, and coplanar quarter elliptical ground planes. A wide operating band of 12.12 GHz (4.58‐16.7 GHz) for S 11 ≤ ? 10 dB is achieved along with an overall antenna footprint of 15.7 × 11.4 mm2. In addition, other desirable characteristics, that is, omnidirectional radiation patterns, peak gain upto 5 dB, and fidelity factor more than 75% are achieved. A good agreement exists between the simulation and measured results. The obtained results illustrate that this antenna has wide operating range and compact dimensions than available structures. 相似文献
163.
Facial Expression Recognition (FER) is an important subject of human–computer interaction and has long been a research area of great interest. Accurate Facial Expression Sequence Interception (FESI) and discriminative expression feature extraction are two enormous challenges for the video-based FER. This paper proposes a framework of FER for the intercepted video sequences by using feature point movement trend and feature block texture variation. Firstly, the feature points are marked by Active Appearance Model (AAM) and the most representative 24 of them are selected. Secondly, facial expression sequence is intercepted from the face video by determining two key frames whose emotional intensities are minimum and maximum, respectively. Thirdly, the trend curve which represents the Euclidean distance variations between any two selected feature points is fitted, and the slopes of specific points on the trend curve are calculated. Finally, combining Slope Set which is composed by the calculated slopes with the proposed Feature Block Texture Difference (FBTD) which refers to the texture variation of facial patch, the final expressional feature are formed and inputted to One-dimensional Convolution Neural Network (1DCNN) for FER. Five experiments are conducted in this research, and three average FER rates 95.2%, 96.5%, and 97% for Beihang University (BHU) facial expression database, MMI facial expression database, and the combination of two databases, respectively, have shown the significant advantages of the proposed method over the existing ones. 相似文献
164.
The aim of this study was to investigate the structure and corrosion resistance of amorphous, amorphous‐crystalline, and crystalline Mg67Zn29Ca4 alloy for biodegradable applications. This paper presents a preparation method and results of the structural characterization and corrosion resistance analysis of the material. Samples were prepared in the form of 3 mm diameter rods. The structure of the alloy was examined with the use of X‐ray diffractometry and scanning electron microscopy. The thermal properties of the samples were examined with differential scanning calorimetry (DSC). Results of DSC analysis were used to determine heat treatment temperatures, allowing to obtain different fractures of crystalline phase in the material. Corrosion resistance of heat‐treated samples was investigated by immersion tests and electrochemical measurements performed in the simulated body fluid. The X‐ray diffraction results confirmed that the prepared Mg67Zn29Ca4 alloy's structure is fully amorphous. After heat treatment, samples with different fractions of amorphous phase in the structure were obtained. Immersion tests of the samples showed that the structure significantly influenced corrosion resistance in examined materials. It should be pointed out, that certain amounts of crystalline phase in amorphous matrix can greatly improve the corrosion resistance of Mg67Zn29Ca4 alloy. 相似文献
165.
为了解决被动雷达系统中的多发射源定位问题,提出了一种基于多重信号分类(MUSIC)算法和图像膨胀(IE)算法的直接定位方法。该方法结合了谱分析中的MUSIC思想,通过对接收量测协方差矩阵进行特征分析求解目标的位置。首先,在目标个数未知的前提下,利用Akaike信息准则(AIC)来确定模型阶数;然后,推导了基于MUSIC的定位代价函数;之后,利用图像膨胀算法处理得到的代价函数平面;最后,膨胀处理后的输出为目标个数及目标位置的估计值。提出的算法有效地解决了目标检测及提取的问题,能够确定多个目标的位置坐标,为后续的定位性能分析提供可能性,也保证了算法的完整性。进一步地分析了多个临近目标情况下影响目标提取性能的主要因素。 相似文献
166.
针对谱聚类融合模糊C-means(FCM)聚类的蛋白质相互作用(PPI)网络功能模块挖掘方法准确率不高、执行效率较低和易受假阳性影响的问题,提出一种基于模糊谱聚类的不确定PPI网络功能模块挖掘(FSC-FM)方法。首先,构建一个不确定PPI网络模型,使用边聚集系数给每一条蛋白质交互作用赋予一个存在概率测度,克服假阳性对实验结果的影响;第二,利用基于边聚集系数流行距离(FEC)策略改进谱聚类中的相似度计算,解决谱聚类算法对尺度参数敏感的问题,进而利用谱聚类算法对不确定PPI网络数据进行预处理,降低数据的维数,提高聚类的准确率;第三,设计基于密度的概率中心选取策略(DPCS)解决模糊C-means算法对初始聚类中心和聚类数目敏感的问题,并对预处理后的PPI数据进行FCM聚类,提高聚类的执行效率以及灵敏度;最后,采用改进的边期望稠密度(EED)对挖掘出的蛋白质功能模块进行过滤。在酵母菌DIP数据集上运行各个算法可知,FSC-FM与基于不确定图模型的检测蛋白质复合物(DCU)算法相比,F-measure值提高了27.92%,执行效率提高了27.92%;与在动态蛋白质相互作用网络中识别复合物的方法(CDUN)、演化算法(EA)、医学基因或蛋白质预测算法(MGPPA)相比也有更高的F-measure值和执行效率。实验结果表明,在不确定PPI网络中,FSC-FM适合用于功能模块的挖掘。 相似文献
167.
针对多角度下车辆出现一定的尺度变化和形变导致很难被准确识别的问题,提出基于多尺度双线性卷积神经网络(MS-B-CNN)的车型精细识别模型。首先,对双线性卷积神经网络(B-CNN)算法进行改进,提出MS-B-CNN算法对不同卷积层的特征进行了多尺度融合,以提高特征表达能力;此外,还采用基于中心损失函数与Softmax损失函数联合学习的策略,在Softmax损失函数基础上分别对训练集每个类别在特征空间维护一个类中心,在训练过程中新增加样本时,网络会约束样本的分类中心距离,以提高多角度情况下的车型识别的能力。实验结果显示,该车型识别模型在CompCars数据集上的正确率达到了93.63%,验证了模型在多角度情况下的准确性和鲁棒性。 相似文献
168.
针对工业控制系统传统单一检测算法模型对不同攻击类型检测率和检测速度不佳的问题,提出一种优化支持向量机和K-means++算法结合的入侵检测模型。首先利用主成分分析法(PCA)对原始数据集进行预处理,消除其相关性;其次在粒子群优化(PSO)算法的基础上加入自适应变异过程避免在训练的过程中陷入局部最优解;然后利用自适应变异粒子群优化(AMPSO)算法优化支持向量机的核函数和惩罚参数;最后利用密度中心法改进K-means算法与优化后的支持向量机组合成入侵检测模型,从而实现工业控制系统的异常检测。实验结果表明,所提方法在检测速度和对各类攻击的检测率上得到明显提升。 相似文献
169.
Changmo Kim Izhar Livne‐Bar Karsten Gronert Jeremy M. Sivak 《Molecular nutrition & food research》2020,64(4)
Lipoxins (LXs) are autacoids, specialized proresolving lipid mediators (SPMs) acting locally in a paracrine or autocrine fashion. They belong to a complex superfamily of dietary small polyunsaturated fatty acid (PUFA)–metabolites, which direct potent cellular responses to resolve inflammation and restore tissue homeostasis. Together, these SPM activities have been intensely studied in systemic inflammation and acute injury or infection, but less is known about LX signaling and activities in the central nervous system. LXs are derived from arachidonic acid, an omega‐6 PUFA. In addition to well‐established roles in systemic inflammation resolution, they have increasingly become implicated in regulating neuroinflammatory and neurodegenerative processes. In particular, chronic inflammation plays a central role in Alzheimer's disease (AD) etiology, and dysregulated LX production and activities have been reported in a variety of AD rodent models and clinical tissue samples, yet with complex and sometimes conflicting results. In addition, reduced LX production following retinal injury has been reported recently by the authors, and an intriguing direct neuronal activity promoting survival and homeostasis in retinal and cortical neurons is demonstrated. Here, the authors review and clarify this growing literature and suggest new research directions to further elaborate the role of lipoxins in neurodegeneration. 相似文献
170.
《Intermetallics》2015
Raney-type Ni precursor alloys containing 75 at.% Al and doped with 0, 0.75, 1.5 and 3.0 at.% Ti have been produced by a gas atomization process. The resulting powders have been classified by size fraction with subsequent investigation by powder XRD, SEM and EDX analysis. The undoped powders contain, as expected, the phases Ni2Al3, NiAl3 and an Al-eutectic. The Ti-doped powders contain an additional phase with the TiAl3 DO22 crystal structure. However, quantitative analysis of the XRD results indicate a far greater fraction of the TiAl3 phase is present than could be accounted for by a simple mass balance on Ti. This appears to be a (TixNi1−x)Al3 phase in which higher cooling rates favour small x (low Ti-site occupancy by Ti atoms). SEM and EDX analysis reveal that virtually all the available Ti is contained within the TiAl3 phase, with negligible Ti dissolved in either the Ni2Al3 or NiAl3 phases. 相似文献