基于JPEG的SAR图像压缩的C语言实现

在对通用软件的提炼、修改基础上，用标准C语言实现基于JPEG的SAR图像数的压缩，实验结果证明相对其它可实现SAR图像压缩的方法，JPEG压缩性能最佳，同时硬件也容易实现。     

Backscattered electron imaging of the undersurface of resin-embedded cells by field-emission scanning electron microscopy

In this study backscattered electron (BSE) imaging was used to display cellular structures stained with heavy metals within an unstained resin by atomic number contrast in successively deeper layers. Balb/c 3T3 fibroblasts were cultured on either 13-mm discs of plastic Thermanox, commercially pure titanium or steel. The cells were fixed, stained and embedded in resin and the disc removed. The resin block containing the cells was sputter coated and examined in a field-emission scanning electron microscope. The technique allowed for the direct visualization of the cell undersurface and immediately overlying areas of cytoplasm through the surrounding embedding resin, with good resolution and contrast to a significant depth of about 2 μm, without the requirement for cutting sections. The fixation protocol was optimized in order to increase heavy metal staining for maximal backscattered electron production. The operation of the microscope was optimized to maximize the number of backscattered electrons produced and to minimize the spot size. BSE images were collected over a wide range of accelerating voltages (keV), from low values to high values to give ‘sections' of information from increasing depths within the sample. At 3–4 keV only structures a very short distance into the material were observed, essentially the areas of cell attachment to the removed substrate. At higher accelerating voltages information on cell morphology, including in particular stress fibres and cell nuclei, where heavy metals were intensely bound became more evident. The technique allowed stepwise ‘sectional’ information to be acquired. The technique should be useful for studies on cell morphology, cycle and adhesion with greater resolution than can be obtained with any light-microscope-based system.     

Linear microstrip surface coil for MR imaging of the rat spinal cord at 4.7 T

A new design of RF coil based on a quasi-transverse electromagnetic field is described. The coil was developed for the acquisition of MR images of the rat spinal cord at 4.7 T. Different materials for the construction of the coil were tested, and the best results were obtained with Teflon. The design of the microstrip coil enables the investigator to change the length of the coil in a longitudinal direction and yields a relatively high signal-to-noise ratio due to the restricted field of view. Low RF field penetration depth also helps in suppressing motion artifacts generated by, e.g., breathing or heartbeats.     

脑显像剂IMP的放射性碘标记及动物实验

文章报道了对本校合成的RS-N-Isopropyl-P-Iodoamphetamine进行~(125)I标记和动物实验的初步结果。我们采用水热法进行同位素交换反应,在Cu(II)和过量还原剂存在的条件下,可以方便地获得高标记率的~(125)I-IMP。经萃取纯化后,放化纯度>98%;游离~(125)I<1%。测定了在大鼠体内静脉注射~(125)I-IMP的分布,结果表明,~(125)I-IMP具有脑摄取率高、脑与血的比值大、在脑贮留时间长等优点,是一种有用的新型脑显象剂。     

Research Profiling of Nano-enchanced Molecular Imaging Technology

Molecular imaging, as an emerging technique in current medical diagnostics, therapy and biology science, has received more and more attention in academia, industry, as well as medical practices. And the applications of nanotechnology in this field stimulate its development. This paper explores the development of the basic research and applied research on nano-enhanced molecular imaging, and does the in-depth analysis by adopting the bibliometrics method. The results show that the US is the leader in this field, who has the absolute advantages. Germany, UK and France and Japan are the followed ones. The rapidly developing Asian countries , China and South Korea might become the potential competitors. Research network analysis draws the conclusion that the other countries and regions share the same research interests with the US. The US not only take the lead in the researching number, but also directing the future orientation of this field. However, the US has not much international collaboration. The collaboration within the country and regions show that collaboration in this field is limited to the regional adjacency. Disciplinary analysis reveals that the major subjects which relate intimately to the nano-enhanced molecular imaging are chemistry, biomedical science and material science.     

Localizable imaging study for the noninvasive diagnosis of lesions with the backscattering polarized light

The asymmetry appearing in the degree of polarization (DOP) distribution of the backscattering polarized light from tissues is investigated by using polarized Monte Carlo simulation. When the incident point is close to the boundary of the lesion inside the tissue, high asymmetry emerges regardless of the polarized direction of the incident light. A noninvasive method based upon the DOP asymmetry of the backscattering light is proposed to locate lesions hidden in live tissues by scanning a point light source. Imaging of the front projection on complicated lesion structures is demonstrated with this method. Its transverse resolution, which is affected by the wavelength of incident light and the size of the scattering particle, can reach the diameter of the lesion scattering particle theoretically while the best longitudinal detection depth can be achieved by choosing a suitable incident wavelength according to the scattering characters of the tissue.     

Multiplexed-Based Assessment of DNA Damage Response to Chemotherapies Using Cell Imaging Cytometry

The current methods for measuring the DNA damage response (DDR) are relatively labor-intensive and usually based on Western blotting, flow cytometry, and/or confocal immunofluorescence analyses. They require many cells and are often limited to the assessment of a single or few proteins. Here, we used the Celigo^® image cytometer to evaluate the cell response to DNA-damaging agents based on a panel of biomarkers associated with the main DDR signaling pathways. We investigated the cytostatic or/and the cytotoxic effects of these drugs using simultaneous propidium iodide and calcein-AM staining. We also describe new dedicated multiplexed protocols to investigate the qualitative (phosphorylation) or the quantitative changes of eleven DDR markers (H2AX, DNA-PKcs, ATR, ATM, CHK1, CHK2, 53BP1, NBS1, RAD51, P53, P21). The results of our study clearly show the advantage of using this methodology because the multiplexed-based evaluation of these markers can be performed in a single experiment using the standard 384-well plate format. The analyses of multiple DDR markers together with the cell cycle status provide valuable insights into the mechanism of action of investigational drugs that induce DNA damage in a time- and cost-effective manner due to the low amounts of antibodies and reagents required.     

一种针对图像模糊的无参考质量评价指标

在成像模型的基础上,分析了图像模糊的原因,提出了一种为图像构造参考图像的方法,进而将结构相似度(SSIM)评价方法引入到无参考图像质量评价中,提出一种无参考结构清晰度(NRSS)的新的无参考图像质量评价方法,将其用于对模糊图像的质量评价。该方法通过低通滤波器来构造参考图像,通过计算原始图像与参考图像的结构相似度值来评价原始图像质量,很好地结合了成像系统的数学模型和结构相似度评价方法的优势,实验结果表明无参考结构清晰度评价指标能够给出和主观评价方法以及其余有参考评价方法一致的结果。