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排序方式: 共有7046条查询结果,搜索用时 15 毫秒
41.
Michael Hausmann Martin Falk Charlotte Neitzel Andreas Hofmann Abin Biswas Theresa Gier Iva Falkova Dieter W. Heermann Georg Hildenbrand 《International journal of molecular sciences》2021,22(7)
In cancer therapy, the application of (fractionated) harsh radiation treatment is state of the art for many types of tumors. However, ionizing radiation is a “double-edged sword”—it can kill the tumor but can also promote the selection of radioresistant tumor cell clones or even initiate carcinogenesis in the normal irradiated tissue. Individualized radiotherapy would reduce these risks and boost the treatment, but its development requires a deep understanding of DNA damage and repair processes and the corresponding control mechanisms. DNA double strand breaks (DSBs) and their repair play a critical role in the cellular response to radiation. In previous years, it has become apparent that, beyond genetic and epigenetic determinants, the structural aspects of damaged chromatin (i.e., not only of DSBs themselves but also of the whole damage-surrounding chromatin domains) form another layer of complex DSB regulation. In the present article, we summarize the application of super-resolution single molecule localization microscopy (SMLM) for investigations of these structural aspects with emphasis on the relationship between the nano-architecture of radiation-induced repair foci (IRIFs), represented here by γH2AX foci, and their chromatin environment. Using irradiated HeLa cell cultures as an example, we show repair-dependent rearrangements of damaged chromatin and analyze the architecture of γH2AX repair clusters according to topological similarities. Although HeLa cells are known to have highly aberrant genomes, the topological similarity of γH2AX was high, indicating a functional, presumptively genome type-independent relevance of structural aspects in DSB repair. Remarkably, nano-scaled chromatin rearrangements during repair depended both on the chromatin domain type and the treatment. Based on these results, we demonstrate how the nano-architecture and topology of IRIFs and chromatin can be determined, point to the methodological relevance of SMLM, and discuss the consequences of the observed phenomena for the DSB repair network regulation or, for instance, radiation treatment outcomes. 相似文献
42.
Analysis of damage localization for ductile metal in process of shear band propagation 总被引:3,自引:0,他引:3
王学滨 《中国有色金属学会会刊》2006,16(1):153-158
1 Introduction Shear localization is an important and often dominating deformation and failure mechanism for Ti and Ti alloy in dynamic loadings[1—10]. The eventual outcome of localized deformation is ductile rupture and material separation. Shear locali… 相似文献
43.
非理想虹膜图像往往存在虹膜边缘模糊、灰度变化不均匀、位置偏移及光斑干扰等问题, 这些问题的存在会在一定程度上影响虹膜内、外边界定位的准确率. 针对这个问题, 本文提出采用并查集和边缘检测模板的方法来对非理想虹膜进行内、外边界定位. 内边界定位是首先采用并查集方法完成瞳孔区域粗定位, 然后采用Hough变换对瞳孔进行精确定位; 外边界定位是先利用一系列边缘检测模板大致确定外圆的位置, 然后再依据外边界附近圆环内边缘点的密度来最终完成外边界的精确定位. 实验结果表明, 对于非理想虹膜图像, 本文方法的定位正确率和定位速度均高于其他同类方法. 相似文献
44.
Many paramedics' work accidents are related to physical aspects of the job, and the most affected body part is the low back. This study documents the trunk motion exposure of paramedics on the job. Nine paramedics were observed over 12 shifts (120 h). Trunk postures were recorded with the computer-assisted CUELA measurement system worn on the back like a knapsack. Average duration of an emergency call was 23.5 min. Sagittal trunk flexion of >40° and twisting rotation of >24° were observed in 21% and 17% of time-sampled postures. Medical care on the scene (44% of total time) involved prolonged flexed and twisted postures (∼10 s). The highest extreme sagittal trunk flexion (63°) and twisting rotation (40°) were observed during lifting activities, which lasted 2% of the total time. Paramedics adopted trunk motions that may significantly increase the risk of low back disorders during medical care and patient-handling activities. 相似文献
45.
The garment fit played an important role in protective performance, comfort and mobility. The purpose of this study is to quantify the air gap to quantitatively characterize a three-dimensional (3-D) garment fit using a 3-D body scanning technique. A method for processing of scanned data was developed to investigate the air gap size and distribution between the clothing and human body. The mesh model formed from nude and clothed body was aligned, superimposed and sectioned using Rapidform software. The air gap size and distribution over the body surface were analyzed. The total air volume was also calculated. The effects of fabric properties and garment size on air gap distribution were explored. The results indicated that average air gap of the fit clothing was around 25–30 mm and the overall air gap distribution was similar. The air gap was unevenly distributed over the body and it was strongly associated with the body parts, fabric properties and garment size. The research will help understand the overall clothing fit and its association with protection, thermal and movement comfort, and provide guidelines for clothing engineers to improve thermal performance and reduce physiological burden. 相似文献
46.
Chemical properties of femoral gland secretions in the desert iguana,Dipsosaurus dorsalis 总被引:4,自引:0,他引:4
Allison C. Alberts 《Journal of chemical ecology》1990,16(1):13-25
This study investigates the chemistry of femoral gland secretions in the desert iguana,Dipsosaurus dorsalis (Lacertilia: Iguanidae), and discusses their possible functional significance. Electrophoretic and proton NMR studies indicated that the secretions are composed of approximately 80 % protein and 20% lipid material. Individual differences in polyacrylamide gel banding patterns of femoral gland proteins were found. Reflectance spectroscopy revealed that the secretions strongly absorb longwave ultraviolet light, a feature that may be important in the localization of secretion deposits in the environment. 相似文献
47.
由于子区域分割粒度的限制,基于阶次序列的定位算法(Sequence-based localization,SBL)存在边缘区域节点定位误差较大和不能保证平均定位误差性能的问题。针对这些问题,提出了一种基于SBL和APIT的混合定位算法,利用APIT信标三角形切割SBL算法子区域,减小子区域面积,实现了分割粒度的细化。通过预先进行系统训练,优化了混合算法的加权系数,进一步提升了算法的定位精度。仿真结果表明,相比于原算法,所提出的混合算法有效地提升了边界区域节点的定位精度,其平均定位误差降低了17.9%,使基于阶次序列的定位算法的定位精度得到了有效改善。 相似文献
48.
49.
EVALUATION OF A METHOD FOR MEASURING MIXING TIME USING NUMERICAL SIMULATION AND EXPERIMENTAL DATA 总被引:10,自引:0,他引:10
Martin Lund n Olle Stenberg Bengt Andersson 《Chemical Engineering Communications》1995,139(1):115-136
A method for measuring mixing performance in large-scale vessels is investigated. The method is evaluated experimentally in a pilotscale reactor and by using a model based on the three-dimensional calculation of the flow field. The method is based on injection of a tracer and measuring the concentration with several detectors at different locations. The mixing performance is measured as the decay rate of the difference between the concentration at the detectors and the final concentration.
The model predicts a strong dependence on injection point. The assumption of a first-order decay rate of the concentration deviation is more appropriate for some injection points. 相似文献
The model predicts a strong dependence on injection point. The assumption of a first-order decay rate of the concentration deviation is more appropriate for some injection points. 相似文献
50.
Three-dimensional simulation using fixed coarse-grid thermal-fluid scheme and conduction heat transfer scheme in distinct element method 总被引:1,自引:0,他引:1
Yoshiyuki Shimizu 《Powder Technology》2006,165(3):140-152
Three-dimensional simulation using particle-thermal-fluid coupling scheme with a mixed Lagrangian-Eulerian approach is presented and the scheme is described by extending author's previous research [Y. Shimizu, 2004. “Fluid Coupling in PFC2D and PFC3D”, numerical modeling in micromechanics via particle methods—2004, Proceeding of 2nd International PFC Conference, Balkema, 2004, pp. 281-287]. The scheme solves the continuity, Navier-Stokes (N-S) and thermal energy equations numerically in Eulerian Cartesian coordinates, then derives pressure, velocity vectors and temperature for each fixed cell by considering particle existence. On the other hand, driving forces and thermal energy from fluid are applied to the particles as external body forces and a source term in the force-displacement law and the heat transfer calculations, respectively. This paper describes the thermal scheme for both fluid and particles. Results of two- and three-dimensional simulations involving forced convective heat transfer by fluid and conduction heat transfer within particles are presented. 相似文献