11S球蛋白酶解产物功能特性及对猪肉肠品质的影响研究

以中豆36为原料,提取11S球蛋白,用胰蛋白酶进行水解。研究不同水解度酶解产物的乳化性、持水性、持油性等功能特性,比较不同水解度产物添加到猪肉肠中对其质构及得率等的影响。结果表明水解度为12%时,11S球蛋白酶解产物的乳化活性及乳化稳定性最高并且对肉肠质构特性和得率影响最佳。     

转谷氨酰胺酶催化对不同大豆蛋白凝胶性的影响

研究转谷氨酰胺酶对大豆分离蛋白和7S、11S球蛋白凝胶特性的影响,采用TA-XT plus物性测定仪、荧光分光光度计对各参数进行测定。结果表明：转谷氨酰胺酶能够显著提高大豆蛋白凝胶的凝胶强度,最佳工艺条件为酶添加量40U/g、温度40℃、pH7.5、作用时间2.5h,但此时凝胶表面疏水性和保水性有所下降。经转谷氨酰胺酶催化后,不同蛋白形成热处理凝胶的凝胶特性均发生显著变化,凝胶强度均显著增加,转谷氨酰胺酶催化后大豆蛋白凝胶强度的顺序为11S＞7S＞SPI。     

体外多酶级联反应体系高效合成莱鲍迪苷M

为建立体外多酶级联反应体系高效合成莱鲍迪苷M（Rebaudioside M,RM）,该文分别构建了含有甜叶菊来源的糖基转移酶UGT76G1和水稻来源的糖基转移酶EUGT11以及拟南芥来源的蔗糖合成酶SUS1的重组大肠杆菌,将甜菊苷经一锅法催化合成RM。为提升该体系中限速酶EUGT11的酶活,从而提升整个体外多酶级联反应体系的RM产量,本文将该酶表达质粒的T7启动子核心区域串联,并比较一重、二重、三重和四重启动子启动转录时该酶活性,当三重启动子启动转录时,重组酶EUGT11的酶活力最高,是一重启动子启动转录时的2.13倍,在酶活力配比为SUS1:UGT76G1:EUGT11/3 copies of T7=1:2:6.39的体外多酶级联反应体系中,RM产量为3.79 mmol/L,较酶活力配比为SUS1:UGT76G1:EUGT11=1:2:3的体外多酶级联反应体系中,RM的产量提升了2.35倍。该文成功建立了高效合成RM的体外多酶级联反应体系,为此后工业化酶法合成RM提供理论和数据支持。     

Relative Frequencies of PAX6 Mutational Events in a Russian Cohort of Aniridia Patients in Comparison with the World’s Population and the Human Genome

Genome-wide sequencing metadata allows researchers to infer bias in the relative frequencies of mutational events and to predict putative mutagenic models. In addition, much less data could be useful in the evaluation of the mutational frequency spectrum and the prevalent local mutagenic process. Here we analyzed the PAX6 gene locus for mutational spectra obtained in our own and previous studies and compared them with data on other genes as well as the whole human genome. MLPA and Sanger sequencing were used for mutation searching in a cohort of 199 index patients from Russia with aniridia and aniridia-related phenotypes. The relative frequencies of different categories of PAX6 mutations were consistent with those previously reported by other researchers. The ratio between substitutions, small indels, and chromosome deletions in the 11p13 locus was within the interval previously published for 20 disease associated genomic loci, but corresponded to a higher end due to very high frequencies of small indels and chromosome deletions. The ratio between substitutions, small indels, and chromosome deletions for disease associated genes, including the PAX6 gene as well as the share of PAX6 missense mutations, differed considerably from those typical for the whole genome.     

豫豆-25 11S球蛋白凝胶性的研究Ⅱ.加热时间和中性盐的影响

研究了加热时间和中性盐对豫豆 2511S球蛋白凝胶质构特性的影响.结果表明:随着加热时间的延长,凝胶的硬度、脆度、粘性增加,但30min后增加趋势不明显,豫豆 2511S球蛋白要形成成熟的、质构性能优良的凝胶,加热时间以大于30min为宜;NaCl对酸性凝胶与碱性凝胶的影响有很大的差异.     

智能配电网多源信息集成模型系统研究

作为智能电网建设发展的重要技术支撑,智能配电网多源信息集成模型融合了配电网现有的自动化系统,并结合IEC 61968-11或IEC 61907-301相关规范标准,通过构建统一智能配电网信息集成互事模型,将智能配电网系统中所涉及输电、变电、配电、用电,以及电源环节的设备资源信息、子系统间点对点服务信息、能源供需信息、电力客户需求侧电能供应响应信息等作为集成模型的信息源,实现了配电网不同子系统间多源信息数据的一体化建模和智能集成共享,使配电网能够满足未来分布式新能源接入、复杂网络化架构、节能环保等智能电网多源数据信息集成模型组件的更高需求.     

嵌入式电镦机控制系统设计

针对传统电镦机控制系统的存在问题,设计以S3C44B0X微处理器及μC/OS-Ⅱ嵌入式操作系统为平台的新型电热镦粗控制系统,完成了数据采集、过程控制以及人机交互等任务.本系统与传统的电热镦粗控制系统相比,具有自动化程度高、控制精度高、操作人性化及文件管理等优势.     

支持向量机在面部神经麻痹检测中的应用

通过提取标准人脸正面图像的鼻尖、眼角、鼻角、嘴角等特征点,构成11维特征向量,在介绍支持向量机(SVM)基本原理和实现算法的基础上,通过SVM对大样本的11维特征向量进行学习,将面部神经麻痹的图像从正常人脸图像中分离出来,为医生诊断提供依据。试验证明,该方法对检测面部神经麻痹的准确率是令人满意的。     

安全中间件之公共安全操作平台

安全中间件是为解决安全的复杂性而引入中间件思想、技术提出的,它与中间件一样,采用了分层的体系架构,从不同的多个层面屏蔽现实生活中所遇到的复杂安全问题,既是平台又是接口,既是框架又是模式解决方案。本文讨论了安全中间件的核心层一公共安全操作平台,以公钥密码加密标准PKCS#11展开设计,强调协作、多平台、多任务的支持。     

One-pot synthesis of the highly efficient bifunctional Ni-SAPO-11 catalyst

Hydroisomerization of linear alkanes to branched isomers is an important petrochemical process for production of gasoline with high octane number.Non-noble metal bifunctional catalysts used in this process always suffer from low metal dispersion and poor metal-acid synergy.Herein,a facile one-pot synthesis method was used to simultaneously regulate metal particle sizes and acidity of the Ni-SAPO-11 hydroisomerization catalyst.The physicochemical properties are investigated using XANES,EXAFS,TEM/STEM,FT-1R,XPS,UV-vis and NH3-TPD.Apart from the highly dispersed nickel nanoparticles with an average diameter of 8 nm,the framework Ni2+ions are generated via substituting framework Al3+ions of the SAPO-11.The formed NiP-OH structures have lower deprotonation energy(DPE)than the SiAl-OH ones,contributing more and stronger acid sites to the Ni-SAPO-11 catalyst.The great metal-acid synergy including high metal to acid sites ratio(nNi/nA)and close intimacy is obtained for the Ni-SAPO-11 catalyst.The Ni-SAPO-11 catalyst outperforms the counterpart prepared by the impregnation method and exhibits comparable activity and isomers selectivity to the Pt/SAPO-11 catalyst in the n-hexane hydroisomerization.