山西翼城冶南汉代冶铁遗址初步调查

冶南遗址位于山西省临汾市翼城县冶南村,面积约1.2 km²,考古年代为西汉。采用X射线荧光分析仪(EDX)、扫描电镜及能谱分析仪(SEM-EDS)、金(矿)相显微镜等对冶南遗址采集的6个炉渣和2个金属铁块进行了基体成分和显微组织检测分析。结果表明,6个炉渣样品为硅钙铝系低铁渣;2个铁块样品为熟铁,可能是生铁炒钢产品。判断该遗址为生铁冶炼遗址,同时也是生铁炒钢遗址。该遗址是目前山西地区确认的第一处大型生铁冶炼及炒钢的汉代官铁遗址。     

电厂厂址选择及厂区总布置与传统环境学的关系

电厂厂址选择及厂区总平面布置设计需考虑各种各样的因素,其中在厂址自然条件方面的因素是和我国传统环境学相通的,文章试图对厂址选择及总布置与传统环境学之间存在的关系加以探讨,希望对电厂设计有所帮助.     

广西容县古代田辽岭冶铜遗址初步研究

田辽岭冶铜遗址位于广西容县容西乡西山村,面积约0.12 km2,考古年代属汉唐时期。对取自该遗址的18个炉渣运用扫描电子显微镜及能谱分析（SEM-EDS）方法进行成分分析;对其中2个炉渣样品采用变形法进行熔点测定。检测结果表明,该遗址采炼铜铅多金属矿石,使用“氧化矿—铜”工艺冶炼红铜。该遗址冶炼温度高,铜渣分离较好,该遗址冶炼技术比较成熟。     

国家潮流能试验场选址决策指标体系与分析方法

国家潮流能试验场是潮流能开发利用技术领域的海上公共试验与服务平台。目前国内尚没有海上试验场的建设经验,系统的选址决策标准仍处于空白阶段。本文在对与试验场建设相关的影响因素进行分析与归纳总结的基础上,提出了国家潮流能试验场选址决策的指标体系和分析方法。通过选址方法的研究,对丰富完善国家海洋能试验场标准体系、加快推进国家海洋能试验场建设具有重要意义。     

论泾河东庄水库灰岩坝址渗漏问题

通过对泾河东庄水库灰岩坝址工程地质钻探成果特别是钻孔压水试验及水文地质成果包括区域水质、水位、水温等不连通性的分析研究,并结合流量测定三维渗漏数值模拟分析,对坝区渗漏问题进行分析和论证,进一步证明在泾河下游东庄灰岩段建坝是可行的,不会发生严重渗漏,为项目的设计、审查、决策方面参考,以促进工程前期工作的进展。     

安徽泾县新四军军部旧址纪念馆设计

在泾县新四军军部旧址纪念馆的方案创作中,设计者贯彻可持续发展的设计理念,针对场地、气候、自然光三方面阐述了创作过程.     

The Role of Heparan Sulfate in CCL26-Induced Eosinophil Chemotaxis

Proinflammatory chemokine ligand 26 (CCL26, eotaxin-3) mediates transendothelial cell migration of eosinophils by binding and activating the G-protein-coupled (GPC) chemokine receptor 3 on the surface of eosinophilic cells. Here we have investigated the role of glycosaminoglycans (GAGs) as potential co-receptors in the process of CCL26-induced eosinophil chemotaxis. For this purpose, we have first identified the GAG-binding site of CCL26 by a site-directed mutagenesis approach in the form of an alanine screening. A panel of GAG-binding-deficient mutants has been designed, generated, and analyzed with respect to their binding affinities to heparan sulphate (HS) by isothermal fluorescence titration studies. This showed that basic amino acids in the α-helical part of CCL26 are strongly involved in GAG-binding. In chemotaxis experiments, we found that decreased GAG-binding affinity correlated with decreased chemotactic activity, which indicates an involvement of GAGs in eosinophil migration. This was further proven by the negative impact of heparinase III treatment and, independently, by the incubation of eosinophils with an anti heparan sulfate antibody. We finally investigated eosinophils’ proteoglycan (PG) expression patterns by real-time PCR, which revealed the highest expression level for serglycin. Including an anti-serglycin antibody in CCL26-induced eosinophil migration experiments reduced the chemotaxis of these immune cells, thereby proving the dependence of eosinophil mobilization on the proteoglycan serglycin.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

天生桥一级水电站施工总布置研究

天生桥一级水电站采用合同管理方式建设，主体工程划分为四个土建及金属结构安装合同和一个厂房永久机电设备安装工程合同，为满足合同管理的要求，进行了场内外交通运输设计、施工场地划分、公用施工临时设施和专用施工临时设施的划分、土石方平衡规划等设计工作，潢足了工程建设的需要     

多道脉冲幅度分析器改进研究

分析了多道脉冲幅度分析器忙时间的形成特点,设计出来一套能减小系统忙时间的方案,从而大大地减少由于忙时间造成的脉冲漏计数。同时分析了改进后系统和实际测量时存在误差的主要因素;该方案具有实现有成本小,方便灵活等优点。