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In order to observe intracellular structures by scanning electron microscopy, excess cytoplasmic matrix must be removed from the fractured surface of cells. Previously we reported an Osmium-DMSO-Osmium method devised for this purpose. This method is very effective in revealing intracellular structures, but requires osmium tetroxide for initial fixation with some consequent disadvantages. In the present study, a revised Osmium-DMSO-Osmium method is reported, in which an aldehyde mixture is used as the initial fixative instead of osmium tetroxide. As fixation is carried out by perfusion in this revised method, better preservation of fine structures is achieved than by the original method, especially in the central nervous tissue which tends to suffer from post-mortem degeneration. Moreover this method can be applied to cytochemical studies of intracellular structures with a scanning electron microscope (SEM). In this study, acid phosphatase of lysosomes is demonstrated in a coloured SEM micrograph. 相似文献
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针对齿形已经确定的摆线轮,在修正方式及修正量未知的情况下,利用改进的复合形法对摆线轮齿形修正方式及修正量进行了优化分析.同时,采用工艺过程简单的“等距+ 移距” 修正方式代替工艺过程较复杂的转角修正方式,使用该方法计算等距和移距修正量速度快,与传统的计算方法相比较,工作效率明显提高.计算结果表明:在摆线轮齿形的各种修正方式中,“正等距+ 负移距”修正方式是较理想的摆线轮齿形修正方式.这种修正方式具有理想齿形,加工工艺简单,在设计与制造中有一定的推广使用价值 相似文献
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周晓霞 《吉林化工学院学报》2009,26(1):31-33
以四种不同的酮类化合物及草酸二乙酯为原料,设计合成了四种3(5)-取代-1H-吡唑甲酸化合物,通过IR及^1H NMR对所合成的化合物结构进行了表征.化合物3a与金属铜离子配位,得到一个双核铜配合物晶体,该晶体属三斜晶系,空间群为P-1,晶胞参数为a=7.082(8)A,b=9.156(10)A,c=25.86(3)A,a=97.088(19)°,β=92.024(18)°,γ=112.649(13)°,V=1529(3)A3,Z=4,Dc=1.597g/cm^3,配合物的晶体结构进一步确证了所合成化合物结构的准确性. 相似文献
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《Journal of Nuclear Science and Technology》2013,50(12):1029-1032
Methods for suppression and control of the axisymmetric vertical positional instability are described on the basis of conceptural design studies with the International Tokamak Reactor (INTOR). The shell effect is well known to be useful for suppression of the fast instability. Here shell structures are presented and their usefulness is assessed. Included in the assessments are required modellings of the plasma current for determination of mutual inductances between the plasma and the inductive components around it. Three kinds of models are described and their features are compared to the shell effect. Two kinds of simplified reactor structures are described and studied by comparing them with some control characteristics of the slow instability by a pair of control coils. Their structures are selected so as to result in different shell effects between them, and are consequently found to produce different capacities of power supply, too. 相似文献
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Immediate recall for spatial information was studied as a function of stimulus load under 2 display formats, 2 response formats, and 2 response set conditions. 4 groups of 10 Ss each served under 15 replications of all response-format, stimulus-load conditions; groups were distinguished on the basis of display format and set. Each S viewed either a spatial or tabular display of 14-26 geometrical stimuli for 16 sec.; he was then required to report, on either a tabular or spatial response form, the location of relevant stimuli. Correct responses and misplacement errors increased more rapidly for the spatial format as more stimuli were presented. Recoding from 1 display to the other response format did not yield serious decrements. Contrary to expectation, response set enhanced all conditions to a nearly equivalent degree. Results are interpreted in terms of the "chunking" hypothesis. (16 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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ThermodynamicStudyontheRareEarthVapourComplexPrAl3Cl12WangLinshan(王林山),GaoRongjie(高荣杰),SuYi(苏轶),WangZhichang(王之昌)(Department... 相似文献