A tilting device for three-dimensional microscopy: Application to in situ imaging of interphase cell nuclei

The resolution of an optical microscope is considerably less in the direction of the optical axis (z) than in the focal plane (x-y plane). This is true of conventional as well as confocal microscopes. For quantitative microscopy, for instance studies of the three-dimensional (3-D) organization of chromosomes in human interphase cell nuclei, the 3-D image must be reconstructed by a point spread function or an optical transfer function with careful consideration of the properties of the imaging system. To alleviate the reconstruction problem, a tilting device was developed so that several data sets of the same cell nucleus under different views could be registered. The 3-D information was obtained from a series of optical sections with a Zeiss transmission light microscope Axiomat using a stage with a computer-controlled stepping motor for movement in the z-axis. The tilting device on the Axiomat stage could turn a cell nucleus through any desired angle and also provide movement in the x-y direction. The technique was applied to 3-D imaging of human lymphocyte cell nuclei, which were labelled by in situ hybridization with the DNA probe pUC 1.77 (mainly specific for chromosome 1). For each nucleus, 3-D data sets were registered at viewing angles of 0°, 90° and 180°; the volumes and positions of the labelled regions (spots) were calculated. The results also confirm that, in principle, any angle of a 2p geometry can be fixed for data acquisition with a high reproducibility. This indicates the feasibility of axiotomographical microscopy of cell nuclei.     

直接还原现状和对我省发展的意见

介绍了国内外直接还原铁的现状，并提出了我省发展直接还原铁的意见 。     

有线电视系统的节目防复制技术

防止电影被录象机复制是在有线电视系统中放映新电影的一个重要问题，特别是在按次收费（ＰＰＶ）业务上。由于存在复制威胁，电影制片厂不愿过早地在有线电视网上放映新电影，这样又反过来影响有线电视收视率。针对这一问题，介绍了伪同步ＡＧＣ方法、彩色条方法和时基变化方法等几种防复制技术，重点介绍了一种基于时基变化方法的有线电视防复制系统。     

浅析古典园林的美学思想（二）

中国古典园林无疑是人类文明的珍贵遗产，不仅体现在其艺术形式和完整成熟的技巧，更体现在其本身所蕴涵着的丰富美学思想。这些思想不但给中国古典园林注入了强劲的生命力，同时也为它的发展和完善提供了坚实的基础和充沛的源泉。因此，探寻这些美学思想与园林形式、造园技巧的关系，就成为了古典园林研究的一个重点。     

Structural schema integration with full and partial correspondence using the dual model

The integration of views and schemas is an important part of database design and evolution and permits the sharing of data across complex applications. The view and schema integration methodologies used to date are driven purely by semantic considerations, and allow integration of objects only if that is valid from both semantic and structural view points. We discuss a new integration method called structural integration that has the advantage of being able to integrate objects that have structural similarities, even if they differ semantically. This is possible by using the object-oriented Dual Model which allows separate representation of structure and semantics. Structural integration has several advantages, including the identification of shared common structures that is important for sharing of data and methods.     

Human resources management and firm performance: The differential role of managerial affective and continuance commitment.

In this study, the authors developed a dual-concern (i.e., maintenance and performance) model of human resources (HR) management. The authors identified commonly examined HR practices that apply to the middle manager level and classified them into the maintenance- and performance-oriented HR subsystems. The authors found support for the 2-factor model on the basis of responses from 2,148 managers from 463 firms operating in China. Regression results indicate that the performance-oriented HR subsystems had a positive relationship with firm performance and that the relationship was mediated by middle managers' affective commitment to the firm. The maintenance-oriented HR subsystems had a positive relationship with middle managers' continuance commitment but not with their affective commitment and firm performance. This study contributes to the understanding of how HR practices relate to firm performance and offers an improved test of the argument that valuable and firm-specific HR provide a source of competitive advantage. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Orbital view concept applied on photoswitching systems

Diarylethenes are a class of photochromic molecules whose conductance switches with their photoisomerization. We have investigated the conductance of diarylethenes using non-equilibrium Green's function method combined with Hückel method (NEGF–HMO) and density functional theory (NEGF–DFT). In this study we have found that the qualitative predictions based on frontier orbital analysis are consistent with the DFT calculations and can be used for prediction of the electron transport properties of molecular devices.     

综合地震技术在深层油气勘探中的应用

深层勘探是中原油田的勘探重点与战略接替方向，深层地震勘探是其中的一个技术难点。为此，在东濮地区做了大量的研究分析，主要研究应用的技术包括：深层气地震采集技术、处理技术和解释技术。通过两年来的研究，在前8井获得了深层油气勘探的巨大突破，展示了前梨园洼陷深层良好的油气勘探前景，并扩大了杜桥白油气叠加连片的场面，拓宽了东濮凹陷深层油气勘探领域。     

美中不同的“东西”——浅谈中西哲理美学的对比

真正理想的“现代设计”,应该更多的是一种融通古今,关怀人性的思考方式,是多种文化并存的“现代化”体现,是庄子所畅想的“天地与我并生,万物与我为一”的人生境界。通过对比中西方的设计理念及中西文化的起源,我们可以清楚地看出,华夏文化与西方文化的差异是明显的。这一差异,将有助于我们看到设计的心理来源,有助于我们充分吸取两者之间的设计精华。     

蚁群算法在物化视图选择问题中的应用

提出用蚁群算法来解决物理空间约束下的物化视图选择问题，算法中应用信息素的局部和全局更新，并对每次迭代的最优解进行局部搜索，最终得出合理的解决方案。实验结果表明，应用蚁群算法可以有效地解决物化视图选择问题，并且其求解性能优于遗传算法。