Plasma Metabolomics Profile of “Insulin Sensitive” Male Hypogonadism after Testosterone Replacement Therapy

Male hypogonadism is a disorder characterized by low levels of testosterone, but patients can either show normal insulin (insulin-sensitive (IS)) or over time they can become insulin-resistant (IR). Since the two groups showed different altered metabolisms, testosterone replacement therapy (TRT) could achieve different results. In this paper, we analyzed plasma from 20 IS patients with low testosterone (<8 nmol/L) and HOMAi < 2.5. The samples, pre- and post-treatment with testosterone for 60 days, were analyzed by UHPLC and mass spectrometry. Glycolysis was significantly upregulated, suggesting an improved glucose utilization. Conversely, the pentose phosphate pathway was reduced, while the Krebs cycle was not used. Branched amino acids and carnosine metabolism were positively influenced, while β-oxidation of fatty acids (FFA) was not activated. Cholesterol, HDL, and lipid metabolism did not show any improvements at 60 days but did so later in the experimental period. Finally, both malate and glycerol shuttle were reduced. As a result, both NADH and ATP were significantly lower. Interestingly, a significant production of lactate was observed, which induced the activation of the Cori cycle between the liver and muscles, which became the main source of energy for these patients without involving alanine. Thus, the treatment must be integrated with chemicals which are not restored in order to reactivate energy production.     

Interactions between Radiation and One-Carbon Metabolism

Metabolic reprogramming is a hallmark of cancer. Cancer cells rewire one-carbon metabolism, a central metabolic pathway, to turn nutritional inputs into essential biomolecules required for cancer cell growth and maintenance. Radiation therapy, a common cancer therapy, also interacts and alters one-carbon metabolism. This review discusses the interactions between radiation therapy, one-carbon metabolism and its component metabolic pathways.     

Metabolites and Genes behind Cardiac Metabolic Remodeling in Mice with Type 1 Diabetes Mellitus

Metabolic remodeling is at the heart of diabetic cardiomyopathy. High glycemic fluctuations increase metabolic stress in the type 1 diabetes mellitus (T1DM) heart. There is a lack of understanding on how metabolites and genes affect metabolic remodeling in the T1DM heart. We hypothesize that differential expression of metabolic genes and metabolites synergistically influence metabolic remodeling preceding T1DM cardiomyopathy. To test our hypothesis, we conducted high throughput analysis of hearts from adult male hyperglycemic Ins2^+/− (Akita) and littermate normoglycemic Ins2^+/+ (WT) mice. The Akita mouse is a spontaneous, genetic model of T1DM that develops increased levels of consistent glycemic variability without the off-target cardiotoxic effects present in chemically- induced models of T1DM. After validating the presence of a T1DM phenotype, we conducted metabolomics via LC-MS analysis and genomics via next-generation sequencing in left ventricle tissue from the Akita heart. Ingenuity Pathway Analyses revealed that 108 and 30 metabolic pathways were disrupted within the metabolomics and genomics datasets, respectively. Notably, a comparison between the two analyses showed 15 commonly disrupted pathways, including ketogenesis, ketolysis, cholesterol biosynthesis, acetyl CoA hydrolysis, and fatty acid biosynthesis and beta-oxidation. These identified metabolic pathways predicted by the differential expression of metabolites and genes provide the foundation for understanding metabolic remodeling in the T1DM heart. By limited experiment, we revealed a predicted disruption in the metabolites and genes behind T1DM cardiac metabolic derangement. Future studies targeting these genes and metabolites will unravel novel therapies to prevent/improve metabolic remodeling in the T1DM heart.     

沿空掘巷小煤柱侧防瓦斯泄漏技术

针对赵庄煤业沿空掘巷存在的小煤柱侧瓦斯泄漏隐患,制定了"柔性薄喷+注浆"治理方案,采用柔性薄喷材料替代混凝土,喷涂厚度5 mm,有效解决了材料运输和开裂问题,采用高密度浅钻孔和快凝注浆材料,避免了浆液流入采空区。效果考察表明:柔性薄喷材料挂壁性和柔韧性良好,掘巷服务期间小煤柱侧未出现明显瓦斯泄漏。     

Improving Homology-Directed Repair in Genome Editing Experiments by Influencing the Cell Cycle

Genome editing is currently widely used in biomedical research; however, the use of this method in the clinic is still limited because of its low efficiency and possible side effects. Moreover, the correction of mutations that cause diseases in humans seems to be extremely important and promising. Numerous attempts to improve the efficiency of homology-directed repair-mediated correction of mutations in mammalian cells have focused on influencing the cell cycle. Homology-directed repair is known to occur only in the late S and G2 phases of the cell cycle, so researchers are looking for safe ways to enrich the cell culture with cells in these phases of the cell cycle. This review surveys the main approaches to influencing the cell cycle in genome editing experiments (predominantly using Cas9), for example, the use of cell cycle synchronizers, mitogens, substances that affect cyclin-dependent kinases, hypothermia, inhibition of p53, etc. Despite the fact that all these approaches have a reversible effect on the cell cycle, it is necessary to use them with caution, since cells during the arrest of the cell cycle can accumulate mutations, which can potentially lead to their malignant transformation.     

非晶Mg65-xNi21Pr14Agx合金电极的制备及其放电性能

用铜模喷铸法制备了非晶Mg65-x Ni21-Pr14Ag x合金,采用球磨方法细化了合金颗粒。系统研究了不同Ag含量对非晶合金电极充放电行为、放电容量、循环稳定性、高倍率放电等性能的影响规律。结果表明,Ag元素的添加改善了合金电极充/放电性能,随着Ag含量的增加,非晶合金电极的最大放电容量和循环稳定性均呈现先增大后减小的规律,其高倍率放电性能也有所提高,适量Ag的添加可明显改善非晶MgNi-Pr系合金的放电性能。     

Tradeoff Analysis for RMS of Equipment Based Operation Availability and Life Cycle Cost

Reliability, maintainability, supportability (RMS) are the important performance indexes of equipment, which affect not only the availability, but also the life cycle cost (LCC) of the equipment. First discussed is the qualitative relation between reliability and LCC and the costs which may be used to enhance the reliability. Secondly, the factors that affect the maintainability are summarized, and if maintainability is advanced, the trend of the LCC is depicted. Thirdly, the qualitative relation between th...     

Application of Magnetron Sputtering in Fabricating MEMS Microbatteries

With the development of MEMS and the electronic devices's miniaturization and integration, a new kind of power sources that can satisfy the need for high energy density is required. Microbatteries are being researched abroad for their advantages of extreme thinness and long-term power supply. The development of MEMS microbatteries are reviewed and suggestedmagnetron sputtering in fabricating a high-performance microbattery. The technics as annealing are analyzed. The microbattery with a LiNi1-x Cox O2 cathode exhibited stable cycle ability and a high specific discharge capacity, which was attributed to the alloying effect of the LiNiO2and LiCoO2.     

一种新型的IGBT驱动保护电路

介绍一种新型的性能较完善的IGBT驱动保护电路。实验结果表明,该电路能快速、安全、可靠地实现保护功能。     

A distributed deadlock detection algorithm for mobile computing system

The mode of mobile computing originated from distributed computing and it has the un-idempotent operation property, therefore the deadlock detection algorithm designed for mobile computing systems will face challenges with regard to correctness and high efficiency. This paper attempts a fundamental study of deadlock detection for the AND model of mobile computing systems. First, the existing deadlock detection algorithms for distributed systems are classified into the resource node dependent （ RD ） and the resource node independent （RI） categories, and their corresponding weaknesses are discussed. Afterwards a new RI algorithm based on the AND model of mobile computing system is presented. The novelties of our algorithm are that： 1 ） the blocked nodes inform their predecessors and successors simultaneously; 2） the detection messages （agents） hold the predecessors information of their originator; 3 ） no agent is stored midway. Additionally, the quit-inform scheme is introduced to treat the excessive victim quitting problem raised by the overlapped cycles. By these methods the proposed algorithm can detect a cycle of size n within n - 2 steps and with （ n^2- n - 2 ）/2 agents. The performance of our algorithm is compared with the most competitive RD and RI algorithms for distributed systems on a mobile agent simulation platform. Experiment results point out that our algorithm outperforms the two algorithms under the vast majority of resource configurations and concurrent workloads. The correctness of the proposed algorithm is formally proven by the invariant verification technique.