双足型直线超声电机的实验研究

研制了一种新型直线超声电机,首先对其驱动机理进行了探讨,设计了电机的具体结构。其次对振子进行了频率响应实验,模态实验,实验表明电机可有效激励出所需振型。分别测定了电机的频率-速度关系、电压-速度关系、相位-速度关系。电机运行中正反两向运动灵活,工作状态稳定,速度基本与输入电压成正比,可采用调压调速;其最大无负载速度可达76 mm/s,最大输出推力2 N,达到了预期设计的目的。     

Improving Homology-Directed Repair in Genome Editing Experiments by Influencing the Cell Cycle

Genome editing is currently widely used in biomedical research; however, the use of this method in the clinic is still limited because of its low efficiency and possible side effects. Moreover, the correction of mutations that cause diseases in humans seems to be extremely important and promising. Numerous attempts to improve the efficiency of homology-directed repair-mediated correction of mutations in mammalian cells have focused on influencing the cell cycle. Homology-directed repair is known to occur only in the late S and G2 phases of the cell cycle, so researchers are looking for safe ways to enrich the cell culture with cells in these phases of the cell cycle. This review surveys the main approaches to influencing the cell cycle in genome editing experiments (predominantly using Cas9), for example, the use of cell cycle synchronizers, mitogens, substances that affect cyclin-dependent kinases, hypothermia, inhibition of p53, etc. Despite the fact that all these approaches have a reversible effect on the cell cycle, it is necessary to use them with caution, since cells during the arrest of the cell cycle can accumulate mutations, which can potentially lead to their malignant transformation.     

Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene in E. coli

Ethylene is an essential platform chemical with a conjugated double bond, which can produce many secondary chemical products through copolymerisation. At present, ethylene production is mainly from petroleum fractionation and cracking, which are unsustainable in the long term, and harmful to our environment. Therefore, a hot research field is seeking a cleaner method for ethylene production. Based on the model ethylene-forming enzyme (Efe) {"type":"entrez-protein","attrs":{"text":"AAD16440.1","term_id":"4323597","term_text":"AAD16440.1"}}AAD16440.1 (6vp4.1.A) from Pseudomonas syringae pv. phaseolicol, we evaluated five putative Efe protein sequences using the data derived from phylogenetic analyses and the conservation of their catalytic structures. Then, pBAD expression frameworks were constructed, and relevant enzymes were expressed in E. coli BL21. Finally, enzymatic activity in vitro and in vivo was detected to demonstrate their catalytic activity. Our results show that the activity in vitro measured by the conversion of α-ketoglutarate was from 0.21–0.72 μmol ethylene/mg/min, which varied across the temperatures. In cells, the activity of the new Efes was 12.28–147.43 μmol/gDCW/h (DCW, dry cellular weight). Both results prove that all the five putative Efes could produce ethylene.     

The Endolysosomal System: The Acid Test for SARS-CoV-2

This review aims to describe and discuss the different functions of the endolysosomal system, from homeostasis to its vital role during viral infections. We will initially describe endolysosomal system’s main functions, presenting recent data on how its compartments are essential for host defense to explore later how SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) and other coronaviruses subvert these organelles for their benefit. It is clear that to succeed, pathogens’ evolution favored the establishment of ways to avoid, escape, or manipulate lysosomal function. The unavoidable coexistence with such an unfriendly milieu imposed on viruses the establishment of a vast array of strategies to make the most out of the invaded cell’s machinery to produce new viruses and maneuvers to escape the host’s defense system.     

新型混合型限流断路器分析及试验

研究了一种基于高速斥力开关的交直流两用混合型限流断路器方案,该限流断路器在限流过程中先经过一个强迫换流过程,使得触头分离时高速斥力开关上电流减小到较小值,触头分离后再经历一个自然零电压换流过程,将斥力开关电流换流到二极管支路上.重点对限流断路器换流过程进行了理论分析及仿真,分析了其可能出现的4种分断情况.研制了400V/500A限流断路器样机,并进行了不同短路电流上升率的限流试验,验证了理论分析的正确性.     

电力设备全寿命周期成本中故障成本的估算

针对全寿命周期成本中故障成本难预测的问题,根据电力设备累积失效概率符合威布尔(Weibull)模型的特点,运用最小二乘法对Weibull模型参数进行估计,计算出设备的期望寿命,从而建立故障成本估算模型。通过比较分析不可修复设备和可修复设备故障成本的计算方式,针对可修复设备平均寿命的特殊性,引进等劣化理论来揭示可修复设备平均寿命之间的关系,并计算出可修复设备的故障成本。算例验证了该方法的有效性。     

华蟾素注射液对人肝癌HepG-2细胞增殖及周期的影响

Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and cell cycle of human hepatoma HepG-2 cells. Methods: Cell proliferation was assessed by MTT assay, cell cycle distributionwas detected by the flow cytometry (FCM). The expression of Cyclin A, CDK2 mRNA levels were examined by RT-PCR.Quantitative colorimetric assay was used to analyze Cyclin NCDK2 activity in HepG-2 cells. Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose- and time-dependent ways; FCM analysis showed Cinobufacini injection induced cell cycle arrest at S phase; RT-PCR assay showed Cinobufacini injection down-regulated Cyclin A, CDK2expression at mRNA levels; Quantitative colorimetric assay showed Cinobufacini injection deceased Cyclin A/CDK2 activity in HepG-2 cells. Conclusion: Cinobufacini injection can inhibit human hepatoma HepG-2 cells growth, induce cell apoptosis and induce cell cycle arrest at S phase, the mechanism of which might be partly related to the down-regulation of Cyclin A,CDK2 mRNA expression and inhibition of Cyclin A/CDK2 activity.     

浅谈我国绿色建筑几点现状问题及发展策略

通过对我国现阶段绿色建筑建设现状几点问题的审慎、严谨、细致的科学分析,提出符合我国文化背景和社会现状的绿色建筑发展策略,以期促进我国绿色建筑的推广应用。     

电动自行车用无镉铅酸蓄电池的开发

研制了电动自行车用无镉铅酸蓄电池。以14.8 V恒压1.8 A限流充电10 h;且以5 A放电到9 V的循环寿命达到700次以上;实车使用寿命可达到3年。电池内不使用镉,有效地减少了对环境的污染。