混合模式的入侵检测技术研究

针对传统入侵检测所采用模式匹配技术存在的弊端,介绍了协议分析技术在入侵检测中的应用,提出了一种混合模式的入侵检测技术,即混合使用特征匹配模式和协议分析技术,充分发挥两者的长处。研究表明,该模型提高了检测效率,减少了误报和漏报。     

蒲石河抽水蓄能电站计算机监控系统控制流程设计

简述了蒲石河抽水蓄能电站概况,介绍了蒲石河抽水蓄能电站计算机监控系统控制流程的设计原则、结构配置、优先级和特殊点,并简要分析了该控制流程的功能特点。蒲石河抽水蓄能电站计算机监控系统控制流程采用了模块化、灵活性、容错性、优先性、独立性、可扩性和安全可靠性等设计原则,实现了容错功能和控制冗余功能,保证了电厂的安全、高效运行,完全能够满足蒲石河抽水蓄能电站对生产设备全面监视和控制的要求,是一种比较先进、可靠的抽水蓄能电站监控系统控制流程。     

火电厂主、辅机及辅助设备顺序控制系统的发展

回顾国内、外火电厂顺序控制系统的发展,特别介绍３５ａ来我国顺序控制系统经历的起步阶段,以程序控制器（ＰＬＣ）为核心硬件的中期阶段和主、辅机顺序控制系统融入分散控制系统（ＤＣＳ阶段、现场总线控制系统（ＦＣＳ）介入阶段,并对由不同形式组成的顺序控制系统的特点进行评价,展望下一世纪该系统的发展趋势。     

无功、负序及谐波电流检测中的数字滤波器研究

数字滤波器是基于瞬时无功理论的无功、负序及谐波电流检测中一个重要部分,其精度和速度直接影响检测的结果。文中在各种电压和电流情况下对电流检测中所需要的数字滤波器进行分析,得出数字滤波器分类设计的想法,并优化设计出三种适用于不同电压和电流情况的数字滤波器。仿真和实验结果证明所设计的数字滤波器与目前电流检测中使用的数字滤波器相比,在保证精度的同时能有效提高速度,且本身算法的复杂性并未显著增加。     

Repetitive Sequence Barcode Probe for Karyotype Analysis in Tripidium arundinaceum

The barcode probe is a convenient and efficient tool for molecular cytogenetics. Tripidium arundinaceum, as a polyploid wild allied genus of Saccharum, is a useful genetic resource that confers biotic and abiotic stress resistance for sugarcane breeding. Unfortunately, the basic cytogenetic information is still unclear due to the complex genome. We constructed the Cot-20 library for screening moderately and highly repetitive sequences from T. arundinaceum, and the chromosomal distribution of these repetitive sequences was explored. We used the barcode of repetitive sequence probes to distinguish the ten chromosome types of T. arundinaceum by fluorescence in situ hybridization (FISH) with Ea-0907, Ea-0098, and 45S rDNA. Furthermore, the distinction among homology chromosomes based on repetitive sequences was constructed in T. arundinaceum by the repeated FISH using the barcode probes including Ea-0663, Ea-0267, EaCent, 5S rDNA, Ea-0265, Ea-0070, and 45S rDNA. We combined these probes to distinguish 37 different chromosome types, suggesting that the repetitive sequences may have different distributions on homologous chromosomes of T. arundinaceum. In summary, this method provide a basis for the development of similar applications for cytogenetic analysis in other species.     

Binary Sequences Derived from Dickson Permutation Polynomials over Binary Extension Field

In this paper, based on a result of Lidl and Mullen (Mathematical Journal of Okayama University, 1991), the maximum length and the second maximum length that can be attained by cycles of Dickson permutation polynomial (of the first kind) with parameter 1 are studied. Necessary and sufficient conditions for these two lengths to be attained are given, which are connected with Fermat primes and Mersenne primes, respectively. Furthermore, a class of coordinate sequences that maintains a large period is obtained, which is shown to be the coordinate sequences derived from cycles of the second maximum length. Explicit formulas for their periodicity and shift-equivalences are also presented.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

An Antisense Oligonucleotide against a Splicing Enhancer Sequence within Exon 1 of the MSTN Gene Inhibits Pre-mRNA Maturation to Act as a Novel Myostatin Inhibitor

Antisense oligonucleotides (ASOs) are agents that modulate gene function. ASO-mediated out-of-frame exon skipping has been employed to suppress gene function. Myostatin, encoded by the MSTN gene, is a potent negative regulator of skeletal muscle growth. ASOs that induce skipping of out-of-frame exon 2 of the MSTN gene have been studied for their use in increasing muscle mass. However, no ASOs are currently available for clinical use. We hypothesized that ASOs against the splicing enhancer sequence within exon 1 of the MSTN gene would inhibit maturation of pre-mRNA, thereby suppressing gene function. To explore this hypothesis, ASOs against sequences of exon 1 of the MSTN gene were screened for their ability to reduce mature MSTN mRNA levels. One screened ASO, named KMM001, decreased MSTN mRNA levels in a dose-dependent manner and reciprocally increased MSTN pre-mRNA levels. Accordingly, KMM001 decreased myostatin protein levels. KMM001 inhibited SMAD-mediated myostatin signaling in rhabdomyosarcoma cells. Remarkably, it did not decrease GDF11 mRNA levels, indicating myostatin-specific inhibition. As expected, KMM001 enhanced the proliferation of human myoblasts. We conclude that KMM001 is a novel myostatin inhibitor that inhibits pre-mRNA maturation. KMM001 has great promise for clinical applications and should be examined for its ability to treat various muscle-wasting conditions.     

Galaxy Dnpatterntools for Computational Analysis of Nucleosome Positioning Sequence Patterns

Nucleosomes are basic units of DNA packing in eukaryotes. Their structure is well conserved from yeast to human and consists of the histone octamer core and 147 bp DNA wrapped around it. Nucleosomes are bound to a majority of the eukaryotic genomic DNA, including its regulatory regions. Hence, they also play a major role in gene regulation. For the latter, their precise positioning on DNA is essential. In the present paper, we describe Galaxy dnpatterntools—software package for nucleosome DNA sequence analysis and mapping. This software will be useful for computational biologists practitioners to conduct more profound studies of gene regulatory mechanisms.     

刚果盆地钾盐矿床沉积特征及成因探讨

刚果盆地是低纬度重要的成盐盆地,主要由白垩系、新近系和第四系组成。含盐地层赋存于白垩纪地层中部,其下伏陆相碎屑岩沉积,上覆海相碎屑—碳酸盐岩沉积。盐层包括5种沉积层序,盐类矿物析出顺序是:硬石膏—石盐—光卤石—水氯镁石—溢晶石。研究区钾盐矿和石盐矿共生,钾盐矿层有20层,累计平均厚度259.61m,KCl品位大于15%。矿石类型主要为光卤石型,次为钾石盐型。矿石为滨海—泻湖相沉积蒸发岩,其沉积与大西洋裂谷的演化发展密切相关,是白垩纪裂谷发育期间,由初始浓缩沉积的富含卤化物海水运移至次级盆地进一步蒸发沉积形成。