全文获取类型
收费全文 | 219篇 |
免费 | 23篇 |
国内免费 | 1篇 |
专业分类
综合类 | 3篇 |
化学工业 | 113篇 |
金属工艺 | 2篇 |
机械仪表 | 4篇 |
建筑科学 | 2篇 |
能源动力 | 1篇 |
轻工业 | 79篇 |
水利工程 | 2篇 |
无线电 | 4篇 |
一般工业技术 | 7篇 |
冶金工业 | 3篇 |
自动化技术 | 23篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 34篇 |
2021年 | 29篇 |
2020年 | 12篇 |
2019年 | 14篇 |
2018年 | 9篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 9篇 |
2014年 | 12篇 |
2013年 | 9篇 |
2012年 | 5篇 |
2011年 | 7篇 |
2010年 | 9篇 |
2009年 | 6篇 |
2008年 | 8篇 |
2007年 | 5篇 |
2006年 | 4篇 |
2005年 | 6篇 |
2004年 | 15篇 |
2003年 | 13篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 2篇 |
排序方式: 共有243条查询结果,搜索用时 15 毫秒
41.
Although Slavic populations account for over 4.5% of world inhabitants, no centralised, open-source reference database of genetic variation of any Slavic population exists to date. Such data are crucial for clinical genetics, biomedical research, as well as archeological and historical studies. The Polish population, which is homogenous and sedentary in its nature but influenced by many migrations of the past, is unique and could serve as a genetic reference for the Slavic nations. In this study, we analysed whole genomes of 1222 Poles to identify and genotype a wide spectrum of genomic variation, such as small and structural variants, runs of homozygosity, mitochondrial haplogroups, and de novo variants. Common variant analyses showed that the Polish cohort is highly homogenous and shares ancestry with other European populations. In rare variant analyses, we identified 32 autosomal-recessive genes with significantly different frequencies of pathogenic alleles in the Polish population as compared to the non-Finish Europeans, including C2, TGM5, NUP93, C19orf12, and PROP1. The allele frequencies for small and structural variants, calculated for 1076 unrelated individuals, are released publicly as The Thousand Polish Genomes database, and will contribute to the worldwide genomic resources available to researchers and clinicians. 相似文献
42.
Similar to other malignancies, TCGA network efforts identified the detailed genomic picture of skin melanoma, laying down the basis of molecular classification. On the other hand, genome-wide association studies discovered the genetic background of the hereditary melanomas and the susceptibility genes. These genetic studies helped to fine-tune the differential diagnostics of malignant melanocytic lesions, using either FISH tests or the myPath gene expression signature. Although the original genomic studies on skin melanoma were mostly based on primary tumors, data started to accumulate on the genetic diversity of the progressing disease. The prognostication of skin melanoma is still based on staging but can be completed with gene expression analysis (DecisionDx). Meanwhile, this genetic knowledge base of skin melanoma did not turn to the expected wide array of target therapies, except the BRAF inhibitors. The major breakthrough of melanoma therapy was the introduction of immune checkpoint inhibitors, which showed outstanding efficacy in skin melanoma, probably due to their high immunogenicity. Unfortunately, beyond BRAF, KIT mutations and tumor mutation burden, no clinically validated predictive markers exist in melanoma, although several promising biomarkers have been described, such as the expression of immune-related genes or mutations in the IFN-signaling pathway. After the initial success of either target or immunotherapies, sooner or later, relapses occur in the majority of patients, due to various induced genetic alterations, the diagnosis of which could be developed to novel predictive genetic markers. 相似文献
43.
44.
As genomes evolve over hundreds of millions years, the chromosomes become rearranged, with segments of some chromosomes inverted, while other chromosomes reciprocally exchange chunks from their ends. These rearrangements lead to the scrambling of the elements of one genome with respect to another descended from a common ancestor. Multidisciplinary work undertakes to mathematically model these processes and to develop statistical analyses and mathematical algorithms to understand the scrambling in the chromo... 相似文献
45.
Irina Lascu Ana Maria Tnase Piotr Jablonski Iulia Chiciudean Maria Irina Preda Sorin Avramescu Knut Irgum Ileana Stoica 《International journal of molecular sciences》2022,23(22)
Polyhydroxyalkanoates (PHA) are promising biodegradable and biocompatible bioplastics, and extensive knowledge of the employed bacterial strain’s metabolic capabilities is necessary in choosing economically feasible production conditions. This study aimed to create an in-depth view of the utilization of Photobacterium ganghwense C2.2 for PHA production by linking a wide array of characterization methods: metabolic pathway annotation from the strain’s complete genome, high-throughput phenotypic tests, and biomass analyses through plate-based assays and flask and bioreactor cultivations. We confirmed, in PHA production conditions, urea catabolization, fatty acid degradation and synthesis, and high pH variation and osmotic stress tolerance. With urea as a nitrogen source, pure and rapeseed-biodiesel crude glycerol were analyzed comparatively as carbon sources for fermentation at 20 °C. Flask cultivations yielded 2.2 g/L and 2 g/L PHA at 120 h, respectively, with molecular weights of 428,629 g/mol and 81,515 g/mol. Bioreactor batch cultivation doubled biomass accumulation (10 g/L and 13.2 g/L) in 48 h, with a PHA productivity of 0.133 g/(L·h) and 0.05 g/(L·h). Thus, phenotypic and genomic analyses determined the successful use of Photobacterium ganghwense C2.2 for PHA production using urea and crude glycerol and 20 g/L NaCl, without pH adjustment, providing the basis for a viable fermentation process. 相似文献
46.
47.
48.
Dr. Yee Hwee Lim Dr. Fong Tian Wong Wan Lin Yeo Dr. Kuan Chieh Ching Yi Wee Lim Elena Heng Shuwen Chen De‐Juin Tsai Dr. Tsai‐Ling Lauderdale Dr. Kak‐Shan Shia Dr. Ying Swan Ho Dr. Shawn Hoon Dr. Ee Lui Ang Dr. Mingzi M. Zhang Prof. Dr. Huimin Zhao 《Chembiochem : a European journal of chemical biology》2018,19(16):1716-1719
49.
50.
Strerath M Gaster J Summerer D Marx A 《Chembiochem : a European journal of chemical biology》2004,5(3):333-339
We report on significantly increased selectivity of real-time PCR through employment of primer probes that bear hydrophobic 4'C modifications at the 3'-terminal nucleotide. The primer probes were designed to bind the target sequences in such a way that the 3'-terminal nucleotide defines whether a matched or a single mismatched basepair is present depending on the respective target sequence. Several commercially available thermostable DNA polymerases belonging to different DNA polymerase families were tested for their efficacy in discriminating between PCR amplification of matched substrates and duplexes that contain a single mismatch. It turned out that, depending on the 4'C modification and the employed DNA polymerase, significantly increased differentiation between single matches and mismatches could be observed with real-time PCR. The degrees of the observed effects varied with the employed 4'C modification and the sequence context studied. The system is robust enough to work faithfully under several buffer conditions. Our approach should be useful for the direct diagnosis of single nucleotide variations within genes, like single nucleotide polymorphisms or mutations, by PCR without the need for further time- and cost-intensive post-PCR analysis. 相似文献