Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice

Helicobacter pylori (H. pylori) has been identified as a group-1 definite carcinogen. As of yet, there is no available vaccine for this microorganism. Our study aimed to identify antigenic peptides in H. pylori using an in silico proteomic approach, and to evaluate their effectiveness as potential vaccine candidates. Four different peptide sequences were prioritized using the reverse vaccinology, namely, CagA¹, CagA², VacA, and SabA. Peptides emulsified with Freunde’s adjuvant were used to immunize BALB/C mice. Subcutaneously immunized mice were challenged by oral administration of H. pylori. IgG, IgA, IL4, and IL17 were detected in mice sera. Histopathology of the dissected stomach of vaccinated and control mice were assessed using H&E stain. IgG was significantly higher in mice vaccinated with SabA. IL-4 was significantly increased in CagA¹, CagA², VacA, and SabA vaccinated mice compared to the adjuvant group. Additionally, histopathological examination of gastric tissue showed a protective effect in the vaccinated groups compared to adjuvant and PBS groups. Our findings indicate a promising effect of the tested epitopes, particularly the SabA antigen, to induce an immune response against H. pylori.     

乳酸菌改善糖尿病代谢作用机制研究进展

糖尿病已成为危害人类生命健康的慢性疾病之一.寻求效果显著,安全性高,副作用小的治疗方式,是目前治疗糖尿病的发展方向之一.而乳酸菌对改善糖尿病具有较好的效果.本文主要综述了肠道乳酸菌的免疫作用与机制,糖尿病与乳酸菌的关系,以及用乳酸菌改善糖尿病的临床应用,重点介绍了乳酸菌调节血糖的作用机理,包括乳酸菌对肠道黏膜屏障功能、...     

Immunogenicity of kappa-casein and glycomacropeptide

Glycomacropeptide (GMP), arising from the cleavage of κ-casein by chymosin or pepsin, has been correlated with a wide variety of biological activities including immunosuppression capacity, inhibition of pathogen invasion, and induction of satiety. Due to the interest in exploiting such potential of GMP, we aimed at characterizing the immunogenic properties of GMP as an indication of its potential allergenicity. Immunogenicity of κ-casein and GMP were investigated using 2 animal models based on different routes of immunization: 1) mice immunized intraperitoneally or subcutaneously with either κ-casein, polymerized GMP, GMP coupled to the immunogenic carrier ovalbumin, or GMP alone; 2) mice coadministered κ-casein or GMP and cholera toxin. The specific antibody response to GMP was evaluated as well as the antigen-specific T-cell response. The results demonstrated that immunization or feeding with κ-casein induced GMP-specific antibodies, whereas GMP per se lacked immunogenicity independently of the mode of presentation. The size of the presented form of GMP did not influence its immunogenicity. Because the results showed that GMP did not induce a specific T-cell response, we postulate that GMP lacks the ability to stimulate antigen-specific T cells.     

汽轮发电机组振动异常检测的阴性选择算法研究

汽轮发电机组振动是火力发电厂最常见的故障类型之一。从人工免疫原理出发,提出用于检测汽轮发电机组异常振动信号的方法。通过对模拟信号和转子实验台得到的全周碰摩数据,建立S集和故障检测器集,用阴性选择算法进行异常信号检测,获得了较高的检测准确率,证明方法的有效性。     

基于久期缺口模型的商业银行利率风险免疫策略及实证分析

随着国内金融市场自由化改革的逐渐深入,使利率风险日渐凸显,从而上升为影响商业银行绩效的主要风险,如何计量和控制利率风险,并对其进行科学地管理,逐渐成为国内外学术界探讨的重要焦点．文中在国内利率市场化改革稳步推进的背景下,通过分析商业银行存在的利率风险,建立了久期缺口管理模型,并进行实证解析和比照,通过实证对比,运用久期缺口管理技术,并结合国内实际情况,能有效地测度和管理利率风险,最终达到预防和控制国内商业银行利率风险．证明运用久期缺口免疫策略可以使商业银行有效地规避利率风险．     

有向网络中病毒传播和核心免疫的研究

现实网络的有向性使得有向网络能够更准确地模拟显示网络,而当前研究的热点是怎样用最小的代价对网络进行免疫。基于节点连接的不均匀性,提出的目标免疫被认为是最好的免疫策略。针对更符合实际的有向无标度网络,提出了基于双向链接的核心免疫策略。与目标免疫相比,该策略可以使网络获得较好的免疫效果,并且能更好地保持网络的连通性。采用SI病毒传播模型研究病毒爆发初期的情况,在双向网络分别测试了该免疫的性能和网络效率,证明了该免疫的高效性。     

邮件网络免疫策略仿真

通过分析病毒在邮件网络中的传播, 可以为复杂网络病毒传播研究提供依据.作者以交互式邮件网络为基础, 对比分析了现有免疫策略以及基于介数信息的免疫策略对病毒扩散的抑制能力.实验结果表明, 在已知全局信息的情况下, 基于节点介数的免疫策略能更有效地抑制病毒传播.     

聚乳酸纳米颗粒的制备与表征

采用改良沉淀法制备了聚乳酸(PLA)纳米颗粒,采用响应面分析法建立数学模型对工艺条件进行优化. 结果表明,影响PLA颗粒制备的主要因素有PLA质量、油相中乙醇含量和油相体积,以平均粒径最小为目标,得到最优工艺条件为：聚乳酸质量76.82 mg,油相中乙醇含量40%(j),油相体积19.49 mL. 按最优工艺条件所制PLA颗粒的粒径为109.1 nm,多分散系数PDI值为0.084,与预测值偏差较小. PLA纳米颗粒为球形实心颗粒,分散性良好且粒径均一. PLA纳米颗粒能被巨噬细胞摄取,有望作为免疫佐剂.     

杭州市免疫规划信息平台设计与应用

从计划免疫的重要性入手,说明了建立免疫规划信息平台的必要性,并重点介绍了平台的设计要求、构建原则、数据库的选型、安全保障体系以及平台所能实现的各项功能等。     

P2P网络中被动型蠕虫传播与免疫建模

鉴于被动型蠕虫的危害性,对被动型蠕虫进行了深入分析,进而基于平均场法建立了被动型蠕虫的传播模型和免疫模型.基于传播模型和流行病传播学理论推导出进入无蠕虫平衡状态的充分条件,仿真实验证明了该充分条件的正确性.另外,仿真实验还表明,下载率和恢复率是控制蠕虫传播的两个可控的关键参数.在免疫软件被编制出来前,降低下载率和提高恢复率能有效控制被动型蠕虫的传播.