全文获取类型
收费全文 | 8403篇 |
免费 | 724篇 |
国内免费 | 369篇 |
专业分类
电工技术 | 437篇 |
综合类 | 780篇 |
化学工业 | 335篇 |
金属工艺 | 112篇 |
机械仪表 | 500篇 |
建筑科学 | 248篇 |
矿业工程 | 106篇 |
能源动力 | 45篇 |
轻工业 | 223篇 |
水利工程 | 51篇 |
石油天然气 | 77篇 |
武器工业 | 78篇 |
无线电 | 2346篇 |
一般工业技术 | 738篇 |
冶金工业 | 137篇 |
原子能技术 | 59篇 |
自动化技术 | 3224篇 |
出版年
2024年 | 24篇 |
2023年 | 81篇 |
2022年 | 148篇 |
2021年 | 187篇 |
2020年 | 178篇 |
2019年 | 169篇 |
2018年 | 175篇 |
2017年 | 216篇 |
2016年 | 232篇 |
2015年 | 294篇 |
2014年 | 557篇 |
2013年 | 467篇 |
2012年 | 695篇 |
2011年 | 622篇 |
2010年 | 524篇 |
2009年 | 474篇 |
2008年 | 508篇 |
2007年 | 575篇 |
2006年 | 480篇 |
2005年 | 437篇 |
2004年 | 373篇 |
2003年 | 342篇 |
2002年 | 275篇 |
2001年 | 222篇 |
2000年 | 196篇 |
1999年 | 172篇 |
1998年 | 114篇 |
1997年 | 125篇 |
1996年 | 113篇 |
1995年 | 91篇 |
1994年 | 60篇 |
1993年 | 70篇 |
1992年 | 39篇 |
1991年 | 39篇 |
1990年 | 19篇 |
1989年 | 21篇 |
1988年 | 16篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 23篇 |
1984年 | 36篇 |
1983年 | 34篇 |
1982年 | 17篇 |
1981年 | 4篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1976年 | 4篇 |
1964年 | 4篇 |
1959年 | 3篇 |
1957年 | 4篇 |
排序方式: 共有9496条查询结果,搜索用时 15 毫秒
91.
Pharaoh Fellow Mwale Chi-Hsin Lee Peng-Nien Huang Sung-Nien Tseng Shin-Ru Shih Hsin-Yuan Huang Sy-Jye Leu Yun-Ju Huang Liao-Chun Chiang Yan-Chiao Mao Wei-Chu Wang Yi-Yuan Yang 《International journal of molecular sciences》2021,22(8)
Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). Children aged <5 years are the most affected by CA16 HFMD globally. Although clinical symptoms of CA16 infections are usually mild, severe complications, such as aseptic meningitis or even death, have been recorded. Currently, no vaccine or antiviral therapy for CA16 infection exists. Single-chain variable fragment (scFv) antibodies significantly inhibit viral infection and could be a potential treatment for controlling the infection. In this study, scFv phage display libraries were constructed from splenocytes of a laying hen immunized with CA16-infected lysate. The pComb3X vector containing the scFv genes was introduced into ER2738 Escherichia coli and rescued by helper phages to express scFv molecules. After screening with five cycles of bio-panning, an effective scFv antibody showing favorable binding activity to proteins in CA16-infected lysate on ELISA plates was selected. Importantly, the selected scFv clone showed a neutralizing capability against the CA16 virus and cross-reacted with viral proteins in EV71-infected lysate. Intriguingly, polyclonal IgY antibody not only showed binding specificity against proteins in CA16-infected lysate but also showed significant neutralization activities. Nevertheless, IgY-binding protein did not cross-react with proteins in EV71-infected lysate. These results suggest that the IgY- and scFv-binding protein antibodies provide protection against CA16 viral infection in in vitro assays and may be potential candidates for treating CA16 infection in vulnerable young children. 相似文献
92.
Most antibodies currently in use have been selected based on their binding affinity. However, nowadays, antibodies that can not only bind but can also alter the function of cell surface signaling components are increasingly sought after as therapeutic drugs. Therefore, the identification of such functional antibodies from a large antibody library is the subject of intensive research. New methods applied to combinatorial antibody libraries now allow the isolation of functional antibodies in the cellular environment. These selected agonist antibodies have provided new insights into important issues of signal transduction. Notably, when certain antibodies bind to a given receptor, the cell fate induced by them may be the same or different from that induced by natural agonists. In addition, combined with phenotypic screening, this platform allows us to discover unexpected experimental results and explore various phenomena in cell biology, such as those associated with stem cells and cancer cells. 相似文献
93.
Dr. Daisuke Fujiwara Kousuke Mihara Ryo Takayama Yusuke Nakamura Prof. Mitsuhiro Ueda Prof. Takeshi Tsumuraya Prof. Ikuo Fujii 《Chembiochem : a European journal of chemical biology》2021,22(24):3406-3409
Conformationally constrained peptides hold promise as molecular tools in chemical biology and as a new modality in drug discovery. The construction and screening of a target-focused library could be a promising approach for the generation of de novo ligands or inhibitors against target proteins. Here, we have prepared a protein kinase-focused library by chemically modifying helix-loop-helix (HLH) peptides displayed on phage and subsequently tethered to adenosine. The library was screened against aurora kinase A (AurA). The selected HLH peptide Bip - 3 retained the α-helical structure and bound to AurA with a KD value of 13.7 μM. Bip - 3 and the adenosine-tethered peptide Bip - 3 - Adc provided IC50 values of 103 μM and 7.7 μM, respectively, suggesting that Bip - 3 - Adc bivalently inhibited AurA. In addition, the selectivity of Bip - 3 - Adc to several protein kinases was tested, and was highest against AurA. These results demonstrate that chemical modification can enable the construction of a kinase-focused library of phage-displayed HLH peptides. 相似文献
94.
通用电子手册数据库录入软件的关键技术 总被引:1,自引:0,他引:1
文章主要讨论了关于通用电子手册数据库录入软件的组成和功能,较为详细地讨论了实际开发中几个关键技术的解决方案.实际设计完成了通用电子手册数据库录入软件模块,并应用于实际工作中,运行良好. 相似文献
95.
96.
The effectiveness of an active shutter-glasses stereoscopic display (SD) and a passive polarised SD was evaluated in a live robot-teleoperation task and a simulated indirect-vision driving task in various terrains. Overall, participants completed their tasks significantly faster with the SDs in three-dimensional (3D) mode than with the SDs in the baseline 2D mode. They also navigated more accurately with the SDs in 3D mode. When the effectiveness of the two types of SDs was examined separately, results showed that the active shutter-glasses SD resulted in faster responses and task completion times than the passive polarised SD, though most of the differences failed to reach statistical significance. Perceived workload when interacting with the two SD systems did not differ significantly between the active versus passive display types or between the 3D and 2D modes of operation; however, participants reported more severe discomfort after interacting with the passive polarised SD. 相似文献
97.
基于组播传输机制设计并实现了雷达系统网络结构,定义了各种显控协议报文的数据结构以及与之相关的组播地址。针对数据报文中的极坐标数据,提出了一种基于三维优化查找表算法的坐标转换方法。对转换过程中的漏点和重点等问题进行了有效解决,并分析了组播在实现多屏显示和在综合船桥系统中的扩展优势。最终系统通过QT绘制界面,在以XC6SLX100T和DAVINCI DM3730为基础的硬件平台上完成了实际测试,验证了系统的可行性。 相似文献
98.
Aqueous photoresists that are capable of higher resolutions than are currently employed by the cathode ray tube (CRT) industry were developed by combining photoactivators, which activate at shorter UV wavelengths with polyvinylpyrrolidone (PVP). Two photoactivators were synthesized: 4,4′‐diazido‐2,2′‐biphenyl disodium disulfonate (DABP), which has a maximum absorbance at 264 nm, and 4,4′‐diazido‐2,2′‐biphenylethane disodium disulfonate (DABPE), which has a maximum absorbance at 258 nm. The PVP/DABP and PVP/DABPE photoresists successfully imaged a pattern with resolution as small as 4.4 μm with photoactivator concentrations greater than 20% of the PVP concentration. Addition of silane and emulgen greatly improved the performance of the photoresists with more uniform coatings of thicknesses of up to 1.4 μm. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 1637–1644, 2006 相似文献
99.
Jared Bon Christan Grant Josh Imbriani Erik Holbrook 《International Journal of Software and Informatics》2016,10(3)
In this paper, we describe our progress in creating the framework for an
interactive application that allows humans to actively participate in a t-SNE clustering
process. t-SNE (t-Distributed Stochastic Neighbor Embedding) is a dimensionality
reduction technique that maps high dimensional data sets to lower dimensions that can
then be visualized for human interpretation. By prompting users to monitor outlying
points during the t-SNE clustering process, we hypothesize that users may be able to make
clustering faster and more accurate than purely algorithmic methods. Further research
would test these hypotheses directly. We would also attempt to decrease the lag time
between the various components of our application and develop an intuitive approach for
humans to aid in clustering unlabeled data. Research into human assisted clustering can
combine the strengths of both humans and computer programs to improve the results of
data analysis. 相似文献
100.
This paper proposes a tactile display providing both shear and normal feedback to the fingertip for generating three-axis tactile feedback during teleoperation of a surgical robot. The display is composed of five balloons actuated by controlling the pneumatic pressure. The implemented display is 18?mm?×?18?mm?×?15?mm. This size is suitable for mounting the display onto the master controls of a surgical robot. The maximum normal and shear displacements are 2 and 1.3?mm, respectively. The proposed tactile display may provide perceivable stimuli to a human finger pad in all five directions: normal, distal, proximal, radial, and ulnar. This paper also reports on the results of psychophysical measurement of the minimum perceivable movement of the developed tactile display for each of the five directions. 相似文献