真型开关柜模拟缺陷下的局部放电试验研究

由于目前开关柜带电检测数据多为间接状态量,难以直接反映开关柜内部局部放电量的大小,容易造成对缺陷的误判。为此利用真型开关柜搭建了开关柜缺陷模拟试验装置,首先利用该装置模拟针板放电缺陷,采用超声波检测法（AE）、暂态地电位检测法（TEV）、特高频检测法（UHF）与脉冲电流法（PC）的比对找出针板放电的规律,包括带电检测信号的典型图谱以及与局部放电量对应关系。最后针对该装置提出了后续的研究范围。     

悬索桥太阳辐射效应的环境因素影响规律

为研究自然环境下悬索桥太阳辐射效应变化规律以及最不利活载效应与环境温度效应的关系,首先建立了某在建大跨度悬索桥精细化模型,然后对太阳辐射效应的环境因素进行参数敏感性分析,同时研究了太阳辐射效应与最不利活载效应的关系.研究结果表明:悬索桥太阳辐射效应受环境温度影响最大、风速次之.风速对悬索桥太阳辐射效应的影响呈非线性关系;日序数和纬度对太阳辐射效应影响较小,但太阳辐射效应变化规律与纬度变化规律不一致,受多种环境因素影响.最不利活载引起的主梁跨中点挠度数值上是热辐射引起最大挠度的2倍,塔顶偏位数值上与热辐射引起的偏位相等.研究结果可以为悬索桥施工阶段误差分析和健康监测数据的温度影响剔除提供理论依据和技术支撑.     

MRT letter: Nanoscopy of protein colocalization in living cells by STED and GSDIM

We report the use of superresolution fluorescence microscopy for studying the nanoscale distribution of protein colocalization in living mammalian cells. Nanoscale imaging is attained both by a targeted and a stochastic fluorescence on-off switching superresolution method, namely by stimulated emission depletion (STED) and ground state depletion microscopy followed by individual molecular return (GSDIM), respectively. Analysis of protein colocalization is performed by bimolecular fluorescence complementation (BiFC). Specifically, a nonfluorescent fragment of the yellow fluorescent protein Citrine is fused to tubulin while a counterpart nonfluorescent fragment is fused to the microtubulin-associated protein MAP2 such that fluorescence is reconstituted on contact of the fragment-carrying proteins. Images with resolution down to 65 nm prove a powerful new way for studying protein colocalization in living cells at the nanoscale.     

Comparative study of the conditions required to image live human epithelial and fibroblast cells using atomic force microscopy

Successful imaging of living human cells using atomic force microscopy (AFM) is influenced by many variables including cell culture conditions, cell morphology, surface topography, scan parameters, and cantilever choice. In this study, these variables were investigated while imaging two morphologically distinct human cell lines, namely LL24 (fibroblasts) and NCI H727 (epithelial) cells. The cell types used in this study were found to require different parameter settings to produce images showing the greatest detail. In contact mode, optimal loading forces ranged between 2-2.8 x 10(-9) and 0.1-0.7 x 10(-9) (N) for LL24 and NCI H727 cells respectively. In tapping (AC) mode, images of LL24 cells were obtained using cantilevers with a spring constant of at least 0.32 N/m, while NCI H727 cells required a greater spring constant of at least 0.58 N/m. To obtain tapping mode images, cantilevers needed to be tuned to resonate at higher frequencies than their resonance frequencies to obtain images. For NCI H727 cells, contact mode imaging produced the clearest images. For LL24 cells, contact and tapping mode AFM produced images of comparable quality. Overall, this study shows that cells with different morphologies and surface topography require different scanning approaches and optimal conditions must be determined empirically to achieve images of high quality.     

足球现场节目两地联播方案的实验及总结

足球现场直播,具有很强的现场感染力.因此,广播的足球现场直播节目通常具有很高的收听率.但是足球现场两地联播,具有较高的技术要求.介绍了广东广播电视台一次足球现场节目两地联播方案的具体实验过程,并对方案的实施效果和经验进行了总结.     

荷载取值费思量——《荷载规范》学习心得

对《建筑结构荷载规范》的一些条文，从规范确定楼面均布活荷载的原则出发，提出一些看法和建议。     

±800 kV特高压直流输电线路带电作业电位转移特性分析

为保障进行±800 kV输电线路带电作业人员的人身安全,对带电作业人员电位转移特性的研究有助于制定合适的安全防护措施。通过建立作业人员进行电位转移过程中基于流体动力学的棒-板间隙放电模型,仿真得到了人与导线间的空气间隙电场强度变化特性,计算了转移过程中的暂态能量,分析了转移距离为0.3~0.6 m时电位转移能量数值的分布特点。结果表明,不同转移距离下的转移能量均在1.0 J附近,随着转移距离的增大,转移能量有所增加,而转移能量高于1.0 J时,导电手套存在被烧蚀的风险,因此作业人员进行±800 kV输电线路带电作业电位转移宜使用电位转移棒进行电位转移,确保作业人员的人身安全。     

带电清洗剂对绝缘子用硅橡胶的溶胀效应及其影响研究

复合支柱绝缘绝缘子由于具有优异的防污性能,在直流换流站得到了广泛应用,但其在空间电荷和静电效应下积污严重而存在大面积污闪风险。为了评估复合支柱绝缘子带电绝缘清洗的可行性,对硅橡胶在新型绝缘清洗下的溶胀特性及其对硅橡胶的电气性能、力学性能以及憎水性的影响开展了试验分析。研究结果表明:硅橡胶溶胀随着溶胀时间的增加溶胀指数和直径增长率持续增大,浸泡时间20 min时圆形硅橡胶试样的直径增长率为4.7%、溶胀指数为6.8%。硅橡胶的复介电常数、复电容、介质损耗因素随着频率、溶胀指数增大而减小;在10-2~103Hz频域范围内硅橡胶溶胀不会造成介电性能下降。硅橡胶溶胀对憎水性和憎水迁移性无明显影响,干燥洁净硅橡胶溶胀前后接触角θ平均值在92°~98°范围内浮动,人工积污并经过憎水迁移后θ平均值在119°~128°范围内浮动。硅橡胶在发生溶胀后其力学性能小幅度下降,溶胀20 min的其断裂拉伸强度下降2%。试验结果表明硅橡胶在清洗剂中溶胀度受浸润时间影响较大,溶胀效应对憎水性、电气和力学性能影响较小,可通过采用易挥发配方或强对流措施控制清洗剂残留对硅橡胶的影响。     

航空发动机试验网络直播技术研究

提出一种基于C/S构架的"网络直播试验"新概念,主要介绍了系统的组成和数据传输的方法,并展望了新构架下的试验工作模式的应用前景。     

浅谈广播透明直播车的设计与实施

以重庆广电集团新建广播透明直播车为案例,就直播车项目的创新点,透明直播车的车体、车载系统、车载视音频系统的设计等几方面做了详细介绍,以供广播技术同仁参考.