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41.
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Hiroko Ishii Maram H. Zahra Atushi Takayanagi Masaharu Seno 《International journal of molecular sciences》2021,22(4)
Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto-1 antibody was produced and purified. The purified antibody showed affinity to recombinant Cripto-1 at 1.1 pmol and immunoreactivity to cancer tissues and cell lines. The antibody was available to detect the immunoreactivity in tissue microarrays of malignant tumors as well as in Cripto-1 overexpressing cells. Simultaneously, the antibody exhibited the potential to suppress the growth of human colon cancer derived GEO cells overexpressing Cripto-1 with IC50 at approximately 110 nM. The artificially humanized antibody is proposed to be a good candidate to target cancer cells overexpressing Cripto-1. 相似文献
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以银耳为原料, 采用超声波辅助热水浸提法提取银耳多糖, 以多糖提取率为指标, 以超声波功率、提取料液比、提取温度和提取时间为影响因素, 设计单因素试验, 并通过正交试验得到较佳提取工艺。通过用TRPV1(Transient Receptor Potential Vanilloid1)试剂盒检测了小胶质细胞TRPV1的释放水平, 与脂多糖(Lipopolysaccharide, LPS)对照组相比, 不同质量浓度的银耳多糖组(62.5, 250, 1000 μg/mL)均能不同程度地抑制TRPV1的释放量。说明了银耳多糖对于BV2小胶质细胞炎症具有良好的抑制作用。 相似文献
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Natural polysaccharides (NPS) are regarded as biomolecular and structural components for preparing high-performance tough hydrogels. But the one-step fabrication of NPS-containing hydrogels in seconds and the template-free design of complicated high-resolution structures are still significant challenges in this field. To meet these requirements, various NPS-containing tough hydrogels are fabricated and processed into 2D/3D structures via the combination of Ru(bpy)32+-mediated photochemistry and extrusion 3D printing technique. The whole fabrication process is one-step, completed in tens of seconds under visible light irradiation. It is found that the used NPS plays a key role in achieving the fabrication of high-performance structured tough hydrogels. The high reactivity of functional groups in the used NPS can shorten their gelation times. Long rigid chains of the used NPS, their hierarchical assemblies, and contrasting multinetworks benefit from the efficient dissipation of mechanical energy and enhancement of its operational stability. Strong supramolecular interactions enable hydrogel precursors to have high viscosities, therefore providing good controllability to design high-resolution and complicated tough hydrogel structures via extrusion 3D printing. It is anticipated that this straightforward fabrication strategy and findings will open new horizons for NPS-containing materials. 相似文献
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Caroline K. Brennan Marya Y. Ornelas Zi W. Yao Prof. Dr. Jennifer A. Prescher 《Chembiochem : a European journal of chemical biology》2021,22(16):2650-2654
Bioluminescent tools have been used for decades to image processes in complex tissues and preclinical models. However, few distinct probes are available to probe multicellular interactions. We and others are addressing this limitation by engineering new luciferases that can selectively process synthetic luciferin analogues. In this work, we explored naphthylamino luciferins as orthogonal bioluminescent probes. Three analogues were prepared using an optimized synthetic route. The luciferins were found to be robust emitters with native luciferase in vitro and in cellulo. We further screened the analogues against libraries of luciferase mutants to identify unique enzyme-substrate pairs. The new probes can be used in conjunction with existing bioluminescent tools for multi-component imaging. 相似文献
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利用正交设计实验的方法安排了一组Zr-Nb二元合金,考查了两个水平的Nb含量,淬火温度,时效温度,时间以及合金组织状态对耐蚀性能的影响,500℃蒸汽腐蚀结果表明,Nb含量控制在固溶极限范围内Zr-Nb二元含金具有较佳的耐蚀性,而当Nb含量较高时,采用双相区淬火,较高温度长时间时效处理是获得较好耐蚀性的工艺方向。 相似文献
50.
Patricia Muñoz-Escalona Shreyes Melkote Kai Liu 《Journal of Materials Engineering and Performance》2005,14(5):582-590
In recent years, the finite element method (FEM) has become the main tool for simulating the metal cutting process because
research based on trial and error is time consuming and requires high investment. Early studies were done by different investigators.
In this research AISI 52100, hardened steel (62 HRC) was selected for an orthogonal machining process as well as metal cutting
simulation using the software DEFORM-2D. This software is based on a forging process and has been adapted to an orthogonal
machining process. The results of simulated cutting forces were compared with experimental cutting force data to validate
the orthogonal cut simulation. Also, the surface roughness was measured, and the influence of the stress, strain, and temperature
on the surface roughness was studied. 相似文献