Inhibitory effects of pulsed electric fields (PEF) on Penicillum expansum inoculated into sour cherry juice, apricot and peach nectars were determined based on germination tube elongation, spore germination rate, and light and scanning electron microscopy (SEM) observations in this study. After inoculation of juice/nectar samples with P. expansum spores at the level of 10(5)-10(6)cfu/mL, the samples were processed by bench scale PEF pulse generator as a function of differing electric field strengths (0, 13, 17, 20, 23, 27, 30 and 34kV/cm) and processing times (0, 62, 94, 123, 163, 198 and 218mus). Results revealed that with an increase in electric field strength and processing time, germination tube elongation and spore germination rate were completely inhibited. Light and SEM observations revealed considerable morphological alterations in fungal conidia such as cytoplasmic coagulation, vacuolations, shrinkage and protoplast leakage. PEF processing of juice/nectars was demonstrated to be effective in inactivating P. expansum. To our knowledge, this is the first study confirming the inhibitory effects of PEF on germination tube elongation and spore germination rate of P. expansum in fruit juice/nectars. 相似文献
Single molecule (SM) methods are able to resolve structure related dynamics of guest molecules in liquid crystals (LC). Highly diluted small dye molecules on the one hand explore structure formation and LC dynamics, on the other hand they report about a distortion caused by the guest molecules. The anisotropic structure of LC materials is used to retrieve specific conformation related properties of larger guest molecules like conjugated polymers. This in particular sheds light on organization mechanisms within biological cells, where large molecules are found in nematic LC surroundings. This review gives a short overview related to the application of highly sensitive SM detection schemes in LC. 相似文献
Two magnetoresistive manganites, La0.83Sr0.17MnO3 and La0.7Sr0.3MnO3, are synthesized by the environmentally friendly “deposition by aqueous acetate solution (DAAS)” technique. The manganite film has a grain size of 100 nm, and can be processed as thinly as 0.03 μm per layer, while the powder form has a crystallite size of 40 nm. These magnetoresistive materials are shown to be effective and inexpensive electromagnetic interference (EMI) shield for the extremely low frequency (ELF) EM fields. The electrical resistance of manganites is very sensitive to external influences, such as temperature and electromagnetic fields. Both permeability (μ) and conductivity (σ) of manganites tend to increase with increasing applied magnetic field. The manganites have been shown to “react” to field increases in a way that is particularly useful for shielding EMI field fluctuations (e.g., due to current or voltage spikes).
The manganite properties, e.g., crystal structure, film morphology, radiation absorption and reflection, electrical resistivity, and magnetization, etc., have been measured. The ceramic manganites have a metal–insulator transition at 300 K or higher, and are suitable for a room temperature operation. A thin film (approx. 0.1 μm) of La0.83Sr0.17MnO3 was fabricated on a quartz tube or refractory ceramic fiber blanket. Using this thin manganite film, the EMI shielding effectiveness for the measured E-field attenuation is similar to that of a 25 μm thickness of copper tube, aluminum foil, and silver–nickel particle-dispersed paper. The silver–nickel impregnated paper has an EMI shielding effectiveness of 35 dB at 10 kHz, and 15 dB at 60 Hz (or frequency above 1 MHz). The ceramic manganites are chemically inert, thermally stable, and mechanically flexible. They provide low cost EMI shielding against directed energy pulses and may serve as a “signature reduction” barrier. 相似文献