Studies of substrate specificities of lipases from different sources

Although lipases are widely applied in a wide variety of reactions, available information on the factors that are responsible for the substrate specificities of lipases from different sources is scarce. In this paper, nine lipase‐producing microorganism strains were isolated from oil‐containing samples. The properties of these enzymes, including pH optima, temperature optima, thermal stability, and pH stability, vary significantly with the different sources from which these lipases were isolated. The substrate specificities of the nine lipases, including fatty acid and positional specificities, were also studied. The fatty acid specificities of the nine lipases were observably different toward 15 substrates with different carbon chain lengths, different saturation degrees and different side chains. The lipases from S₃ Penicillium citrinum (PCL), MJ₁ Aspergillus niger (ANL), MJ₂ Aspergillus oryzae (AOL), YM Bacillus coughing (BCL), S₉ Geotrichum candidum (GCL), and S₁₁ Candida lypolytica (CLL) showed the strongest specificities to short‐chain esters, and the other lipases showed strong selectivity for medium‐ or long‐chain and branched esters. The positional specificities were examined by analyzing the hydrolytic products of triolein catalyzed by the nine lipases, using TLC. The lipases can be mainly classified into two groups by their specificities for the ester bond at position 2 of triglycerides; one group can selectively hydrolyze the ester bond at position 2 of the triglycerides, the other group cannot. All these nine lipases were divided into four groups by hierarchical clustering analysis on the basis of the results of fatty acid and positional specificities.     

Modern Teaching and Training in Metallurgical Engineering

This contribution deals with advanced educational technologies needed to equip customers from higher education institutions, research and industry with efficient tools supporting their work and operating new skills‐training methods. The challenges are reducing the training costs, improving quality and increasing the number of graduates in engineering departments. The concept of a Virtual Lab based on the combination of various teaching methods and tools is presented. Principles of mathematisation in metallurgical education and training are discussed. An example of online course designed in the form of Virtual Lab is demonstrated.     

Evaluation codes at singular points of algebraic differential equations

We use the special geometry of singular points of algebraic differential equations on the affine plane over finite fields to study the main features and parameters of error correcting codes giving by evaluating functions at sets of singular points. In particular, one gets new methods to construct codes with designed minimum distance. This work was partially supported by MCyT BFM2001-2251.     

D-side: A facility and workforce planning group multi-criteria decision support system for Johnson Space Center

“To understand and protect our home planet, to explore the universe and search for life, and to inspire the next generation of explorers” is NASA's mission. The Systems Management Office at Johnson Space Center (JSC) is searching for methods to effectively manage the Center's resources to meet NASA's mission. D-Side is a group multi-criteria decision support system (GMDSS) developed to support facility decisions at JSC. D-Side uses a series of sequential and structured processes to plot facilities in a three-dimensional (3-D) graph on the basis of each facility's alignment with NASA's mission and goals, the extent to which other facilities are dependent on the facility, and the dollar value of capital investments that have been postponed at the facility relative to the facility's replacement value. A similarity factor rank orders facilities based on their Euclidean distance from Ideal and Nadir points. These similarity factors are then used to allocate capital improvement resources across facilities. We also present a parallel model that can be used to support decisions concerning allocation of human resources investments across workforce units. Finally, we present results from a pilot study where 12 experienced facility managers from NASA used D-Side and the organization's current approach to rank order and allocate funds for capital improvement across 20 facilities. Users evaluated D-Side favorably in terms of ease of use, the quality of the decision-making process, decision quality, and overall value-added. Their evaluations of D-Side were significantly more favorable than their evaluations of the current approach.     

Automatic processing of psychological distance: Evidence from a Stroop task.

A picture-word version of the Stroop task was used to test the automatic activation of psychological distance by words carrying various senses of psychological distance: temporal (tomorrow, in a year), social (friend, enemy), and hypotheticality (sure, maybe). The pictures implied depth, with the words appearing relatively close to or distant from the observer. The participants classified the spatial distance of words faster when the word's implicit psychological distance matched its spatial distance (e.g., a geographically close word was classified faster when it was "friend" than when it was "enemy"). The findings are consistent with the idea that psychological distance is accessed automatically, even when it is not directly related to people's current goals, and suggest that psychological distance is an important dimension of meaning, common to spatial distance, temporal distance, social distance, and hypotheticality. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Linear variational principle for convex vector maximization

For infinitely dimensional convex vector maximization problems, it is proved that there exists, under certain conditions, an arbitrarily small additive perturbation of the performance criterion by a linear continuous operator such that the perturbed problem has efficient solutions. The study was sponsored by the Ukrainian State Fund for Basic Research __________ Translated from Kibernetika i Sistemnyi Analiz, No. 2, pp. 105–114, March–April 2007.     

High‐error approximate dictionary search using estimate hash comparisons

A method for finding all matches in a pre‐processed dictionary for a query string q and with at most k differences is presented. A very fast constant‐time estimate using hashes is presented. A tree structure is used to minimize the number of estimates made. Practical tests are performed, showing that the estimate can filter out 99% of the full comparisons for 40% error rates and dictionaries of up to four million words. The tree is found to be efficient up to a 50% error rate. Copyright © 2006 John Wiley & Sons, Ltd.     

Optimal implementations of UPGMA and other common clustering algorithms

In this work we consider hierarchical clustering algorithms, such as UPGMA, which follow the closest-pair joining scheme. We survey optimal O(n²)-time implementations of such algorithms which use a ‘locally closest’ joining scheme, and specify conditions under which this relaxed joining scheme is equivalent to the original one (i.e. ‘globally closest’).     

Comparative study on adaptive rational macromodels of highly resonant structures

Two macromodeling techniques using rational bases are investigated to accurately predict the natural frequencies of highly resonant microwave structures. Three methods are proposed and compared to calculate pole‐free solutions to the Thiele continued fraction and vector fitting pole‐residue models of the characteristic equation det[Z(s)]. © 2007 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2007.     

单片机在超声测距系统中的一种应用

为提高人们在测量距离时的方便性和准确性,现介绍一种基于单片机控制的超声测距系统的构成、工作原理和硬软件设计,利用该系统及其设计方法可以作为机器人的辅助视觉系统,也可用于其他的检测系统中.