37Ar用于视察地下核试验的尝试

对地下核爆漏气体中放射性核素＾３７Ａｒ进行取样,提纯及测量并研究了泄漏速率随时间位置的变化规律。结果表明：监测土壤中＾３７Ａｒ的含量可有效地核查地下核试验。     

安徽泾县新四军军部旧址纪念馆设计

在泾县新四军军部旧址纪念馆的方案创作中,设计者贯彻可持续发展的设计理念,针对场地、气候、自然光三方面阐述了创作过程.     

The Role of Heparan Sulfate in CCL26-Induced Eosinophil Chemotaxis

Proinflammatory chemokine ligand 26 (CCL26, eotaxin-3) mediates transendothelial cell migration of eosinophils by binding and activating the G-protein-coupled (GPC) chemokine receptor 3 on the surface of eosinophilic cells. Here we have investigated the role of glycosaminoglycans (GAGs) as potential co-receptors in the process of CCL26-induced eosinophil chemotaxis. For this purpose, we have first identified the GAG-binding site of CCL26 by a site-directed mutagenesis approach in the form of an alanine screening. A panel of GAG-binding-deficient mutants has been designed, generated, and analyzed with respect to their binding affinities to heparan sulphate (HS) by isothermal fluorescence titration studies. This showed that basic amino acids in the α-helical part of CCL26 are strongly involved in GAG-binding. In chemotaxis experiments, we found that decreased GAG-binding affinity correlated with decreased chemotactic activity, which indicates an involvement of GAGs in eosinophil migration. This was further proven by the negative impact of heparinase III treatment and, independently, by the incubation of eosinophils with an anti heparan sulfate antibody. We finally investigated eosinophils’ proteoglycan (PG) expression patterns by real-time PCR, which revealed the highest expression level for serglycin. Including an anti-serglycin antibody in CCL26-induced eosinophil migration experiments reduced the chemotaxis of these immune cells, thereby proving the dependence of eosinophil mobilization on the proteoglycan serglycin.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

旧区改造中居民就地安置的可行性分析

动拆迁安置与补偿是当前旧区改造所面临的焦点问题,由此引发的社会矛盾也日益成为影响和谐社会构建和社会经济健康发展的障碍。在不突破国家现行市场经济体制、房屋土地制度及城市房屋拆迁制度的前提下,落实《物权法》及Ⅸ城乡规划法》中维护人权、物权的精神,遵循市场规则实施就地安置可作为旧城改造的可行途径,为此政府应给予适当的政策性扶持,增加开发项目的赢利途径,而城市规划也应致力于研究城市功能优化与重构的综合开发模式。     

一种基于浏览器的CSRF攻击检测方法

互联网的安全形势日益严重,CSRF漏洞是最严重的Web漏洞之一,如何检测CSRF攻击成为业内热点.目前的CSRF检测技术主要通过验证字段例如Form表单字段、验证Same Origin等传统方式,但字段容易被攻击者更改,从而成功绕过CSRF检测.在验证字段的基础上,通过进一步分析网页内容,提出一种基于浏览器的CSRF检...     

大型抽水蓄能机组现场启动调试技术研究及应用

随着抽水蓄能电站建设的不断增加,越来越多的抽水蓄能机组需投入电网运行,为了确保新投入机组的安全、可靠、稳定运行,新投产机组必须经过一整套严格的启动试验程序,以检验机组的安装和制造质量,同时为机组能否进入电网稳定运行作出评价。本文结合工程实际,详细叙述了抽水蓄能机组现场启动调试的步骤和顺序,并就电站首机首次启动方式进行了分析和论证。     

频率响应法研究噻吩和苯在NiY分子筛上的吸附行为

The adsorption behavior of thiophene and benzene on NiY zeolite has been investigated by the frequency response(FR) method.The FR spectra of thiophene and benzene on NiY zeolite were recorded at 302—335 K in a pressure range of 26.6—266 Pa.The results showed that adsorption was found to be the rate-controlling process for the thiophene/NiY zeolite system,and there were two different adsorption processes.Two kinds of adsorption models have been proposed,namely:the S-M interaction(low frequency adsorption) an...     

天生桥一级水电站施工总布置研究

天生桥一级水电站采用合同管理方式建设，主体工程划分为四个土建及金属结构安装合同和一个厂房永久机电设备安装工程合同，为满足合同管理的要求，进行了场内外交通运输设计、施工场地划分、公用施工临时设施和专用施工临时设施的划分、土石方平衡规划等设计工作，潢足了工程建设的需要