Construction of human monoclonal single-chain Fv antibodies against small-cell lung cancer by phage display libraries derived from cell-immunized SCID mice engrafted with human peripheral blood lymphocytes

In this study, we describe a phage display strategy to obtain human monoclonal single-chain Fv (scFv) antibodies binding target cancer cell surface proteins. By developing a cancer cell immunization protocol for SCID mice engrafted with human peripheral blood lymphocytes in combination with an antibody phage display method, we have isolated phage antibodies binding small-cell lung cancer cell line H889 by subtractive selection. One of the isolated scFv antibodies, 12EAb, recognized the E2 component of pyruvate dehydrogenase complex (PDC-E2) by immunoprecipitation according to MALDI-TOF MS analysis. Furthermore, we have confirmed the plasma membrane localization of PDC-E2 in small-cell lung cancer cells by immunocytochemistry and cell surface protein biotinylation, although PDC-E2 is usually located in the mitochondrial matrix. These results, including unique localization of identified antigens, were obtained by proteomic approaches. The present methods can be applied to generate human monoclonal scFv antibodies against tumor cells and to identify new molecular targets for immunotherapy and markers for diagnosis.     

基于Flex的XML数据通信与应用研究

XML是一个精简的SGML,作为一个简单而灵活的标准格式,为基于Web的应用提供了一个描述数据和交换数据的有效手段;而随着Web2．0的升温及RIA的广泛应用与发展,Adobe公司推出的Flex为开发人员构建下一代丰富互联网应用（RIA）提供了无限的可能。本文对XML在Flex中的数据通信与应用作了详细的讨论,并给出了详细的实例分析。     

基于PRO/E的凸轮机构运动仿真及分析

机械产品的运动分析和仿真在机械产品的设计中是不可缺少的重要环节。文章通过凸轮机构的运动仿真设计过程介绍了Pro/E中的仿真模块以及运动分析。     

基于Cell多核处理器的层次化运行时支持技术

基于Cell处理器的异构多核架构及软件显式管理的多级存储层次,使其面临编程困难和性能难以有效发挥等问题.现有基于Cell/B.E.的编程模型多侧重于支持类似于流处理的批量访存(bulk data transfer)应用,传统非规则访存应用性能较低.通过扩展Cell/B.E.访存库增强协处理单元的自主作用,以协处理单元为中心建立Cell计算平台上的MPI和弱一致性Pthread分层并行编程运行时支持.分层的运行时支持结构及扩展后的Cell/B.E.访存库使模型具有更好的效率和可扩展性,并且提高了非规则应用的性能;模型中的MPI方便了大量传统并行应用向新架构的移植及开发,而弱一致性Pthread则为MPI提供高效的任务运行时管理支持及为系统级用户提供对架构全面控制的编程接口.实验结果表明,提出的运行时支持技术不仅可适应不同应用的要求,同时借助访存库中的剖分优化机制可有效地挖掘Cell/B.E.架构性能.     

Guiding students to the right questions: adaptive navigation support in an E‐Learning system for Java programming

Rapid growth of the volume of interactive questions available to the students of modern E‐Learning courses placed the problem of personalized guidance on the agenda of E‐Learning researchers. Without proper guidance, students frequently select too simple or too complicated problems and ended either bored or discouraged. This paper explores a specific personalized guidance technology known as adaptive navigation support. We developed JavaGuide, a system, which guides students to appropriate questions in a Java programming course, and investigated the effect of personalized guidance a three‐semester long classroom study. The results of this study confirm the educational and motivational effects of adaptive navigation support.     

低功耗温室无线测量节点的设计

为了提高温室环境参数的监测效果,采用射频SoC芯片nRF9E5、数字温湿度传感器SHT11及光强度传感器TSL2560D,设计了无线温湿度及光照度监控系统的测量节点;并根据无线通信系统的特点设计了系统的软硬件结构,其低功耗无线传输模式降低了节点的能耗.经调试和试验运行,系统具有可靠性高、使用方便、通信节点容量易扩展等优点,这对较大空间的温湿度及光照度的监控具有良好的应用价值.     

Efficient analysis of wireless communication antennas using an accurate [Z] matrix interpolation technique

An accurate impedance matrix interpolation technique based on the surface integral equation (SIE) is presented for the analysis of wireless communication antennas over wide frequency bands. The first‐order derivative of the impedance matrix at the internal frequency is considered in the cubic polynomial‐based interpolation scheme, thus the novel impedance matrix interpolation scheme will provide high accuracy and high efficiency over a frequency band. To demonstrate the efficiency and accuracy of the proposed method, numerical results for planar inverted F antennas (PIFA) and a wideband E‐shaped patch antenna are presented. Good agreement among the interpolation results, exact MoM solutions, finite element method (FEM) solutions, and measured data is observed over the bandwidth. Besides, dimensions of the feeding probe are also studied to investigate their effect on the input impedance and radiation patterns. © 2010 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2010.     

一种无线传感器监测系统的设计和研究

给出了一种无线传感器监测系统的设计与开发,结合MEMS微传感器和无线通信技术,研究了MEMS微加速度计检测信号的测量与无线传输技术,设计了系统电路,并完成了原型装置的制作与调试。通过无线、有线传感器的时频响应特性比较表明,无线传感器监测系统能够满足桥梁健康监测的要求。     

Volatility dynamics of the US business cycle: A multivariate asymmetric GARCH approach

Most empirical investigations of the business cycles in the United States have excluded the dimension of asymmetric conditional volatility. This paper analyses the volatility dynamics of the US business cycle by comparing the performance of various multivariate generalised autoregressive conditional heteroskedasticity (GARCH) models. In particular, we propose two bivariate GARCH models to examine the evidence of volatility asymmetry and time-varying correlations concurrently, and then apply the proposed models to five sectors of Industrial Production of the United States. Our findings provide strong evidence of asymmetric conditional volatility in all sectors, and some support of time-varying correlations in various sectoral pairs. This has important policy implications for government to consider the effective countercyclical measures during recessions.     

基于龙芯2E的AMC处理器模块设计

介绍基于国产通用处理器的嵌入式高级夹层卡（AMC）处理器模块的设计和实现。该模块以龙芯2ECPU为核心,板卡设计符合AMC规范,可以直接应用于符合新型先进电信计算构架规范的计算机系统。模块配有适用于嵌入式系统互联的串行RapidIO接口,可以提供充足的有效带宽用于数据通信和系统配置。给出模块的芯片组设计和软件配置。