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81.
Dr. Heike M. Kropp Dr. Samra Ludmann Dr. Kay Diederichs Dr. Karin Betz Dr. Andreas Marx 《Chembiochem : a European journal of chemical biology》2021,22(21):3060-3066
With increasing temperature, nucleobases in DNA become increasingly damaged by hydrolysis of exocyclic amines. The most prominent damage includes the conversion of cytosine to uracil and adenine to hypoxanthine. These damages are mutagenic and put the integrity of the genome at risk if not repaired appropriately. Several archaea live at elevated temperatures and thus, are exposed to a higher risk of deamination. Earlier studies have shown that DNA polymerases of archaea have the property of sensing deaminated nucleobases in the DNA template and thereby stalling the DNA synthesis during DNA replication providing another layer of DNA damage recognition and repair. However, the structural basis of uracil and hypoxanthine sensing by archaeal B-family DNA polymerases is sparse. Here we report on three new crystal structures of the archaeal B-family DNA polymerase from Thermococcus kodakarensis (KOD) DNA polymerase in complex with primer and template strands that have extended single stranded DNA template 5’-overhangs. These overhangs contain either the canonical nucleobases as well as uracil or hypoxanthine, respectively, and provide unprecedented structural insights into their recognition by archaeal B-family DNA polymerases. 相似文献
82.
在多维键参数空间中对填隙Ti_2Ni相化合物的形成条件做模式识别分析,由于前过渡金属原子组成的八面体空穴中填入C,N.O原子使Ti_2Ni结构稳定化,此结构的分布区扩大,邻近的Al_2Cu,MoSi_2结构分布区减小。 相似文献
83.
剖析网络评价文本能为景观规划设计提供科学的依据支撑,弥补因快速城镇化建设发展而导致的公众参与不足,对于景观设计而言是非常重要的。以重庆市弹子石老街为例,基于网络文本内容分析法,利用rostcm6、excel及python等分析和数据抓取软件,采集大众点评的相关评论数据,以山地景观设计视角结合相关数据分析软件进行定性定量分析并制作可视化分析图,最终从多维度分析游客评论得出结果及改进建议,总结针对重庆弹子石老街及山地景观设计的6条设计策略:(1)交通可达的组织方式;(2)垂直上下的衔接转换;(3)对景远眺的外向空间;(4)本土文化的特色彰显;(5)城市事件的策划安排;(6)游览夜景的慢行系统,期望对今后的类似山地景观空间规划设计提供一定参考和借鉴价值。 相似文献
84.
A software system of pattern recognition on spectrum signal of metal transfer mode has been developed using Visual Basic under Windows environment. On the basis of the coincidence relation between the spectrum signal and metal transfer mode, according to the geometrical pattern feature of the spectrum signal, several key characteristic parameters are extracted. The correspondent recognition function and a minimum distance classifier have been constructed based on Bayesian decision theory. The results show that using this system, the metal transfer mode of MIG, MAG, CO2 welding can be recognized automatically which provides the basis for automatically controlling of the metal gas arc welding metal transfer. 相似文献
85.
基于电容层析成像和模糊模式识别的油气两相流流型辨识新方法的研究 总被引:4,自引:0,他引:4
基于电容层析成像和模糊模式识别技术别提出了一种油气两相流流型辨识的新方法。建立了12电极电容层析成像流型自动识别系统,该系统利用Tikhonov正则化原理并结合SIRT(Simultaneous Reconstruction Techniques)算法进行图像重建。Tikhonov正则化原理用于克服图像重建过程中的不适定问题,SIRT算法用于提高最终重建图像的质量。根据流型的随机和模糊特性,提出了一种根据管截面重建图像进行流型辨识的模糊流型判别方法。研究结果表明,提出的流型辨识新方法是有效的。对于层状流、核心流、环状流、均相流等流型,流型辨识的准确率高于95%,辨识一个流型所用的时间小于0.3秒。对于塞状流,流型辨识的准确率高于90%。 相似文献
86.
Two parasitic angiosperms,Agalinis purpurea (Scrophulariaceae) andStriga asiatica (Scrophulariaceae), are compared as to the chemical recognition events involved in host selection.Agalinis is a hemiparasite which can mature to seed-set without a host, whereasStriga is a holoparasite and survives for only a very limited time without a host. Both parasites, however, attach to a host through a specialized organ known as the haustorium and regulate the development of this organ through the recognition of chemical factors from host plants. We now describe the discovery of 2,6-dimethoxy-p-benzoquinone (2,6-DMBQ) as an haustoria-inducing principle fromSorghum root extracts. Our investigation of this compound has led us to suggest that one level of host recognition in these parasitic plants is mediated through their enzymatic digestion of the host root surface. Degradation of surface components liberates quinonoid compounds, such as 2,6-DMBQ, which in turn trigger haustorial development. 相似文献
87.
88.
Jason Hochreiter Zhongkai HanSyed Zain Masood Spencer FonteMarshall Tappen 《Image and vision computing》2014
In this paper, we propose an album-oriented face-recognition model that exploits the album structure for face recognition in online social networks. Albums, usually associated with pictures of a small group of people at a certain event or occasion, provide vital information that can be used to effectively reduce the possible list of candidate labels. We show how this intuition can be formalized into a model that expresses a prior on how albums tend to have many pictures of a small number of people. We also show how it can be extended to include other information available in a social network. Using two real-world datasets independently drawn from Facebook, we show that this model is broadly applicable and can significantly improve recognition rates. 相似文献
89.
90.
《Computer Speech and Language》2014,28(6):1287-1297
This paper proposes an efficient speech data selection technique that can identify those data that will be well recognized. Conventional confidence measure techniques can also identify well-recognized speech data. However, those techniques require a lot of computation time for speech recognition processing to estimate confidence scores. Speech data with low confidence should not go through the time-consuming recognition process since they will yield erroneous spoken documents that will eventually be rejected. The proposed technique can select the speech data that will be acceptable for speech recognition applications. It rapidly selects speech data with high prior confidence based on acoustic likelihood values and using only speech and monophone models. Experiments show that the proposed confidence estimation technique is over 50 times faster than the conventional posterior confidence measure while providing equivalent data selection performance for speech recognition and spoken document retrieval. 相似文献