The discovery of biomarkers for type 2 diabetic nephropathy by serum proteome analysis

Diabetic nephropathy (DN) is a serious kidney complication of diabetes, and constitutes the leading cause of end-stage renal disease. The earliest clinical evidence of DN is microalbuminuria, a term which refers to the appearance of small but abnormal amounts of albumin in the urine. However, screening methods for DN, such as biomarker assays, are yet to be developed for type 2 DN. In the present study, in an attempt to identify the biomarkers for initial diagnoses of type 2 DN, the protein profiles of human sera collected from 30 microalbuminuric type 2 diabetic patients were compared with those collected from 30 normoalbuminuric type 2 diabetic patients, via 2-DE. As a result, a total of 18 spots were determined to have different protein levels in the microalbuminuric patients. Twelve spots had lower protein levels of approximately 50%, and the other six had higher levels of approximately 100-300% as compared to the spots of normoalbuminuric patients. These spots were identified with ESI-Q-TOF (ESI-quadrupole-TOF) MS. Among the identified proteins, vitamin D-binding protein (DBP) and pigment epithelium-derived factor (PEDF) were verified by Western blotting. The results of this study indicate that the DBP may be employed as diagnostic and monitoring biomarkers of type 2 DN, contingent on further study into the matter.     

Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS

Urine is a human specimen that is easily obtained non-invasively for clinical diagnosis. We attempted to enhance the resolution of current human urine proteomes and construct a comprehensive reference database for advanced studies, such as the discovery of biomarkers for renal diseases. Multi-dimensional LC-MS/MS was coupled with de novo sequencing and database matching. The proposed approach improved the identification of not only the proteins, but also the post-translational sites of urinary proteins. We identified 165, 200 and 259 unique gene products in the urine proteomes from males, females and pregnant women, respectively. When all of the results were combined and the redundancies removed, a total of 1095 distinct peptides were identified. Of these, 1016 peptides were associated with 334 unique gene products. In this study, over 100 gene products, including some disease-related proteins, were detected in urine for the first time by proteomic approaches. Various proteins with novel post-translational hydroxylation were identified using the MASCOT program and de novo sequencing. All proteins with peptide information were summarized into a comprehensive urine protein database. We believe that this comprehensive urine proteome database will assist in the identification of urinary proteins/polypeptides whose spectra are difficult to interpret in the discovery of urinary biomarkers.     

A base62 transformation format of ISO 10646 for multilingual identifiers

ISO 10646 Universal Character Set (UCS) is a 31‐bit coding architecture that covers symbols in most of the world's written languages. Identifiers in programming languages are usually defined by using alphanumeric characters of ASCII, which represent mainly English words. An approach for working around this deficiency is to encode multilingual identifiers into the alphanumeric range of ASCII. For case‐sensitive languages, an encoding that utilizes [0–9][A–Z][a–z] can be more space‐efficient for multilingual identifiers. This paper proposes a base62 transformation format of ISO 10646 called UTF‐62. The resulting string of UTF‐62 is within a [0–9][A–Z][a–z] range, a total of 62 base characters. UTF‐62 also preserves the lexicographic sorting order of UCS‐4. Copyright © 2001 John Wiley & Sons, Ltd.     

Response to ‘Comments on: A cohesion measure for object‐oriented classes’

Indicating that CBMC does not satisfy the monotonic property in terms of the number of interactions, Xu and Zhou proposed an augmented definition of CBMC by adopting cut set instead of glue methods. The augmented CBMC clearly satisfies the monotonic property. However, CBMC is designed to overcome the problem with respect to the number of interactions and, therefore, focuses on the interaction pattern, especially, member connectivity. Consequently, it does not make sense to mention the monotonic property of CBMC with respect to interaction number. Moreover, the notion of glue methods allows several interpretations on the design quality of a class. However, that meaningful interpretation is not possible for the augmented definition due to the removal of the notion of glue methods. Copyright © 2001 John Wiley & Sons, Ltd.     

High-temperature superconducting microwave devices: Fundamental issues in materials, physics, and engineering

High-T _c superconductivity has generated a great deal of interest because of the challenges it presents in the fields of material science, condensed matter physics, and electrical engineering, and because of the potential applications which may result from these research efforts. Thin-film passive microwave components may become the first high-temperature superconducting (HTS) devices available for widespread use and commercialization. In this article, we review aspects of material science, physics, and engineering which directly impact high-T _c superconducting microwave devices and discuss issues which determine the performance of these devices. Methods of growing HTS thin films on large-area substrates, techniques for fabricating single-level HTS passive microwave components, and the relevant properties of high-T _c superconducting films are discussed, with a focus on thin films of the HTS material YBa₂Cu₃O_7–. Several known mechanisms for microwave loss in both the superconductor and the dielectric substrate are presented. An overview of the general classes of superconducting passive microwave devices is given, and representative microwave devices which have been recently demonstrated are described in detail. Examples of a select few HTS active microwave components are also presented. Potential microwave applications are illustrated with comparisons to current technology.     

New oxide phosphors for FEDs: Ruddlesden—Popper phases synthesized with IIIB‐group elements

Abstract— A red‐emitting phosphor, SrTiO³:Pr³⁺, for low‐voltage‐type FEDs and VFDs was developed by Futaba Corporation in 1996. The addition of Al or Ga is essential in the preparation of this phosphor because it improves the luminescence efficiency dramatically. For this impurity effect, Futaba Corporation proposed a charge‐compensation mechanism, which was supported by a recent observation of emission lines due to Al³⁺‐Pr³⁺ pairs. In addition, it was found that Al also works as a scavenger of planar defects, presumably SrO thin layers interleaved in the SrTiO³ lattice, by forming strontium aluminates. The latter mechanism suggests the possibility that a similar impurity effect can be found in materials with crystal structures, including alkaline‐earth oxide layers (Ruddlesden‐Popper phases).     

Improvement of luminance and luminous efficacy in PDPs

Abstract— The dependence of PDP luminance and efficacy on the input power was investigated for several Xe‐Ne gas mixtures. The input power was varied in two ways: namely, by changing the dielectric‐layer capacitance (thickness) and by changing the sustain voltage. A distinctly different behavior was found; for increasing capacitance the efficacy decreases markedly, whereas for increasing sustain voltage the efficacy increases. A design window comprising the combination of a high Xe concentration and a high sustain voltage was suggested. In this window, a high luminance and a high efficacy are concurrent. A 4‐in. test panel with 10% Xe in Ne has been realized showing a white luminance of 2040 cd/m² and an efficacy of 2.3 lm/W for continuous sustaining at 50 kHz with a sustain voltage of 225 V.     

STABILIZATION OF A CLASS OF NONLINEAR NON‐MINIMUM PHASE SYSTEMS

This paper tackles the problem of stabilization of a class of non‐minimum phase nonlinear systems which have zero dynamics with an eigenvalue zero of multiplicity 2. By adding some new terms, called cross terms, we are able to generalize the concept of the Lyapunov function with a homogeneous derivative along the trajectory, which was introduced in [4], to produce a suitable Lyapunov function. The Lyapunov function assures that the stability of an approximate system, which consists of some lower order terms of a nonlinear system with an eigenvalue zero of multiplicity 2, implies the stability of the whole system. Applying these to non‐minimum phase zero dynamics of nonlinear systems with such a center, a sufficient condition and a design method of state feedback control are obtained for stabilizing the systems.     

H∞ ALMOST DISTURBANCE DECOUPLING FOR MIMO BILINEAR SYSTEMS

The H_∞ almost disturbance decoupling problem is considered. In this paper, a nonlinear design is proposed to find a state feedback controller for bilinear systems. The closed‐loop system is internally stable and achieves disturbance attenuation in nonlinear H_∞ sense. We defined a special form of Lyapunov function, which is constructed in terms of one or a set of positive definite constant matrices. If, except of the origin of system, the corresponding polynomial of the positive definite matrix (or several polynomials relevant to the positive definite constant matrices) has (have) no zero on a given subset of state space, then we can construct a controller to solve our problem. It is found that the controller structure could be complicated, but is feasible in computation and may require optimization technique to search the solution. We consider both SIMO and MIMO cases with illustrated examples.