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71.
José Luis Montañez-Soto Salvador Alegret Juan Alfredo Salazar-Montoya Emma Gloria Ramos-Ramírez 《European Food Research and Technology》2006,223(3):379-386
In this work a novel amperometric biosensor for fructose determination in solutions was developed. The device was constructed by the incorporation of a tetrathiofulvalene-tetracyanoquinodimethane organic conducting salt and fructose dehydrogenase enzyme, include in a polymeric matrix of epoxy resin and graphite powder. Because of the electrocatalytic function of the salt, the direct transfer of the electron between the reduced prosthetic group (PQQH2) of the enzyme and the transducing material, was verified at a low working potential (150 mV vs. Ag/AgCl), where the interfering reactions were minimized. The response time at 90% of the steady state value was less than 20 s. The current response was directly proportional to the D-fructose concentration from 0.01 to 0.3 mmol/l with a detection limit of 0.005 mmol/l (signal/noise of 3) and a sensitivity of 1.9985 μA/mmol. The biosensor sensitivity diminishes when its surface is not polished between successive determinations, and remains constant (rsd=1.85, n=10) when the surface is polished between determinations. The effects of temperature and pH on the biosensor response were studied and analyzed; also the properties of the enzyme (Km
ap, I
max, Q10) were determinate in this work. The biosensor was used to determine fructose in high fructose syrups and there were not significant differences between these results and those obtained by HPLC (p≤0.05). During 4 months, in intermittent determinations the biosensor kept 100% of its original sensitivity and after 18 months stored at 4°C, it only lost 32% of its sensitivity. The simplicity, low working potential, high stability and good performance of this biosensor shows a great potential for its use in the fructose determination. 相似文献
72.
一种PN码自适应捕获门限的改进算法 总被引:1,自引:0,他引:1
文献[1]提出了一种用于直扩系统的PN码自适应门限算法。但该文献也指出,此算法对门限总数十分敏感。当门限总数设置不当时,系统的平均捕获时间将显著增加。这限制了该算法在实际中的应用。本文就此提出了改进,给出了算法及电路框图。仿真结果表明,与原方案相比,改进算法改善了对门限总数的敏感性,降低了PN码平均捕获时间。 相似文献
73.
介绍了EASY2000色谱处理系统在酒类产品分析实际应用过程中的方法,步骤,特点及注意事项等。 相似文献
74.
XRF法测定高岭土中的主次量元素 总被引:6,自引:0,他引:6
采用X射线荧光光谱法取代原有的化学法,分析了高岭土中的9种主次量元素。通过对测定条件的选择以及样品制备等方面的考察,制定出最佳分析方案。分析结果表明,该方法准确、可靠,分析周期由原来的6h缩短为10min. 相似文献
75.
本文讨论静载荷及疲劳载荷作用下 C/Al 正交层压板的力学响应,并考虑损伤过程中的临界现象,以此作为结构设计准则的基础。对静载荷作用的情况:(1)90°铺层的门槛应变ε_(FPF)随着约束程度增加而增加,而且开裂后的行为也受约束程度的影响。(2)经历加载-卸载再重新加载至断裂的过程后,初始试样存在一个剩余永久变形,其损伤状态可以用刚度的变化来表征;而疲劳试样在加载-卸载过程中刚度变化很小,反映其损伤达到一个稳定的状态。对疲劳载荷作用的情况:(1)对于给定的应力比 R,试样会趋于与它所承受的 S_(max)最大值相应的 SDS 状态。循环应力范围的不断增大,会引起基体损伤的增加,从而重新建立新的饱和损伤状态。(2)对于每一损伤状态均有一对应的循环应变范围 Δε。在 SDS 状态下,Δε保持不变。(3)以刚度下降为损伤参数对 C/Al 层压板进行了降级应力分析,并由此来预计在ΔS_(sh)以下,层压板不会发生疲劳损伤累积。 相似文献
76.
I. A. Chaban 《Journal of Superconductivity》1996,9(1):33-42
The impurity mechanism proposed earlier is used to interpret the magnetic properties of hightemperature superconductors. The appearance of superconductive granules and the existence of weak bonds in grains of ceramics and in single crystals is explained. The irreversibility line is obtained and its new interpretation as the line of thresholds of appearance of infinite nonsuperconductive cluster is given. The relaxation time of the magnetic susceptibility in alternating magnetic fields is calculated. The decrease of the Meissner fraction with increase in magnetic field and decrease in doping, and other magnetic properties are explained. 相似文献
77.
Jozef P H Linssen Anneloes L G M Janssens Hanneke C E Reitsma Wender L P Bredie Jacques P Roozen 《Journal of the science of food and agriculture》1993,61(4):457-462
Taste recognition threshold concentrations (TRTC) of styrene were determined in samples of oil-in-water emulsions (30–300 g kg?1 oil) and yoghurts (1–30 g kg?1 fat), spiked with styrene. The observed TRTC increased linearly with increasing fat content and ranged from 0–3 to 2–1 mg kg?1 for the emulsions and from 36 to 171 g kg?1 for the yoghurts. Styrene equilibrium partition coefficients between emulsions and their respective vapour phases were determined. The concentrations of styrene in the continuous aqueous phase of the emulsions and yoghurts were calculated at the TRTC. The styrene concentrations in the continuous phase had constant values of about 15 g kg?1 indicating that perception of styrene for oil-in–water emulsions is determined by the aqueous phase of the emulsion. The concentrations of styrene in the vapor phases above the emulsions and yoghurts were also calculated and were found to be constant at the TRTC. This relationship probably resulted from the equilibrium of distribution of styrene between the respective phases. Commercial yoghurt packed in polystyrene beakers contained styrene levels in the range 2–11 g kg?1, much lower then the TRTC reported. 相似文献
78.
79.
本文对激光光束通过望远系统后光束的变化进行了分析,并对6倍望远镜进行了激光损伤和破坏的实验研究,实验结果表明,激光对望远系统的损伤破坏主要是热效应所致,损伤破坏的情况与激光的能量,波长,脉冲宽度和望远系统的结构有关。 相似文献
80.
A simple and computationally efficient method of determining the transfer function and other characteristics of systems with multiple inputs and multiple outputs (MIMO systems) described by autoregression equations is proposed. The method is based on the use of the discrete Fourier transformation. The algorithm is highly suitable for computer implementation. The efficiency and simplicity of the method are illustrated using the example of a system with three inputs and three outputs. The proposed concept may be applied to systems described by the autoregression sliding mean. 相似文献