Covalent Protein Labeling by SpyTag–SpyCatcher in Fixed Cells for Super‐Resolution Microscopy

Labeling proteins with high specificity and efficiency is a fundamental prerequisite for microscopic visualization of subcellular protein structures and interactions. Although the comparatively small size of epitope tags makes them less perturbative to fusion proteins, they require the use of large antibodies that often limit probe accessibility and effective resolution. Here we use the covalent SpyTag–SpyCatcher system as an epitope‐like tag for fluorescent labeling of intracellular proteins in fixed cells for both conventional and super‐resolution microscopy. We also applied this method to endogenous proteins by gene editing, demonstrating its high labeling efficiency and capability for isoform‐specific labeling.     

Sixteen Polymorphic Simple Sequence Repeat Markers from Expressed Sequence Tags of the Chinese Mitten Crab Eriocheir sinensis

The Chinese mitten crab (Eriocheir sinensis) is an economically important aquaculture species in China. In this study, we developed and evaluated simple sequence repeat markers from expressed sequence tags of E. sinensis. Among the 40 wild E. sinensis individuals tested, 16 loci were polymorphic. The number of alleles per locus ranged from two to ten. The observed heterozygosity ranged from 0.0667 to 0.9667, whereas the expected heterozygosity ranged from 0.0661 to 0.9051. These markers have the potential for use in genetic studies of population structure and intraspecific variation in E. sinensis.     

Aromatic Phosphonates: A Novel Group of Emitters Showing Blue Ultralong Room Temperature Phosphorescence

In recent years, there has been a growing interest in purely organic materials showing ultralong room-temperature phosphorescence with lifetimes in the range of seconds. Still, the longest known phosphorescence lifetimes are only achieved with crystalline systems so far. Here, a rational design of a completely new family of halogen-free organic luminescent derivatives in amorphous matrices, displaying both conventional fluorescence and phosphorescence is reported. Hydrogen bonding between the newly developed emitters and an ethylene-vinyl alcohol copolymer (Exceval) matrix, which efficiently suppresses vibrational dissipation, enables bright long-lived phosphorescence with lifetimes up to 2.6 s at around 480 nm. The importance of the chosen matrix is shown as well as the implementation in an organic programmable luminescent tag.     

Blue‐Light‐Absorbing Thin Films Showing Ultralong Room‐Temperature Phosphorescence

The development of organic materials displaying ultralong room‐temperature phosphorescence (URTP) is a material design‐rich research field with growing interest recently, as the luminescence characteristics have started to become interesting for applications. However, the development of systems performing under aerated conditions remains a formidable challenge. Furthermore, in the vast majority of molecular examples, the respective absorption bands of the compounds are in the near ultraviolet (UV) range, which makes UV excitation sources necessary. Herein, the synthesis and detailed analysis of new luminescent organic metal‐free materials displaying, in addition to conventional fluorescence, phosphorescence with lifetimes up to 700 ms and tailored redshifted absorption bands, allowing for deep blue excitation, are reported. For the most promising targets, their application is demonstrated in the form of organic programmable tags that have been recently developed. These tags make use of reversible activation and deactivation of the URTP by toggling between the presence and absence of molecular oxygen. In this case, the activation can be achieved with visible light excitation, which greatly increases the use case scenarios by making UV sources obsolete.     

Utilizing the multiple facets of WWW contents

Current query languages for the Web (e.g., W3QL, WebLog and WebSQL) explore the structure of the Web. However, usually, the structure of the Web has little to do with the semantics of the data. Therefore, it is practically difficult to pose database queries over the Web. We introduce a new type of tags for denoting the semantics of data stored in HTML pages. These semantic tags (implemented as HTML comments) superimpose on HTML pages semistructured objects in the style of the OEM model. The paper discusses two implemented tools for fully utilizing the semantics. The first is a visualization tool for displaying both the HTML reading of Web pages and the OEM reading of Web pages. The second tool is a query language, similar to LOREL, that can query the HTML structure and/or the OEM reading. The above formalism and tools provide data-modeling capabilities for the Web that fit its heterogeneous nature. Real database queries, taking the OEM point of view, can be formulated, including queries about the schema as well as queries about the HTML structure of Web pages. Therefore, the query language is not restricted to portions of the Web in which semantic tags are used.     

用户权限控制在SSMBSS中的设计与实现

在Web开发中,权限往往是一个极其复杂的问题,针对不同的应用,需要根据项目的实际情况和具体架构,在维护性、灵活性、完整性等多个方面比较、权衡。本文简要介绍了在Struts和Hibernate框架下基于角色的权限管理(RBAC)思想,提出了自定义标签和Struts标签相结合的方式,提高了系统的灵活性和安全性,达到MVC很好的分离。     

常见的RFID标签估算方法及其统计分析

随着射频识别技术在诸多领域的应用,其反碰撞问题也得到了广泛的关注。标签估算作为动态帧时隙ALOHA算法中的关键,其估算精确度对系统识别有着直接的影响。在对常见的标签估算方法进行介绍的基础上,本文通过计算机仿真实验和统计的不同方法,在估算准确度和稳定性等方面进行了分析和对比。     

一种新的RFID传感系统的防碰撞算法的研究

标签碰撞是射频识别RFID传感系统中常见问题,为了解决这个问题提出了一种新的非线性自适应Q值算法.非线性自适应Q值算法是在一次识别标签过程中非线性自适应改变参数Q值大小来解决标签数目不固定,且变化范围很大情况下的防碰撞问题.文章给出了算法的详细流程,并通过ReaderSim程序来仿真验证了算法的正确性.     

标记和数据相融合的网页信息隐藏算法

从网页由标记信息和数据信息两部分组成的结构特点出发,建立了基于标记信息隐藏和基于数据信息隐藏的融合策略,并据此构建了网页信息隐藏算法,该算法提高了网页信息隐藏的隐藏容量,也为载密信息选取网页作为通道的传输方式进行了探索。     

基于LabVIEW的电子防盗标签分拣系统设计

本文简要介绍了虚拟仪器的慨念，叙述了系统的检测原理和数据采集与处理的过程，并利用LabVIEW平台开发电子防盗标签分拣系统。实践证明该系统设计合理，操作方便，完全满足生主要求，并具备较强的可扩充性。