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Renner S Ludwig V Boden O Scheffer U Göbel M Schneider G 《Chembiochem : a European journal of chemical biology》2005,6(6):1119-1125
TAR RNA is a potential target for AIDS therapy. Ligand-based virtual screening was performed to retrieve novel scaffolds for RNA-binding molecules capable of inhibiting the Tat-TAR interaction, which is essential for HIV replication. We used a "fuzzy" pharmacophore approach (SQUID) and an alignment-free pharmacophore method (CATS3D) to carry out virtual screening of a vendor database of small molecules and to perform "scaffold-hopping". A small subset of 19 candidate molecules were experimentally tested for TAR RNA binding in a fluorescence resonance energy transfer (FRET) assay. Both methods retrieved molecules that exhibited activities comparable to those of the reference molecules acetylpromazine and chlorpromazine, with the best molecule showing ten times better binding behavior (IC50 = 46 microM). The hits had molecular scaffolds different from those of the reference molecules. 相似文献
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Global Positioning System (GPS) is currently the most frequently used wayfinding aid for driving. Yet, GPS is designed to act as a driving guide rather than to help users gain spatial knowledge. Accordingly, GPS might be less usable in situations where such knowledge is required or highly desirable. In this study, we experimentally study the influence of GPS display scales (single-scale vs. dual-scale) using simulated driving tasks in a virtual environment. The single-scale GPS is similar to the regular GPS view. The dual-scale GPS aid is a dual-scale navigation tool that provides two levels of detail, including both detailed and contextual information. The results demonstrate that the dual-scale GPS was more efficient in leading the participants to the destination during the simulated driving and was more useful for the participants to establish spatial awareness and a cognitive map; the dual-scale GPS participants also reported higher subjective evaluations. The proposed dual-scale GPS design and experimental results show some indications for designing new wayfinding aids aimed at increasing wayfinding performance while simultaneously helping users construct a cognitive map. 相似文献
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Engagement in virtual worlds has become pervasive, particularly among the young. At the same time, the number of virtual environments has increased rapidly. Due to intensifying competition, promoting sustained usage, i.e. continuance, has become a top priority for virtual world operators. Prior research has shown that network externalities play a key role in the adoption of communication technologies. However, a small amount of research has examined the role of network externalities in continued IT usage in general or with respect to the virtual world participation in particular. To fill in this gap, we examine how perceived network externalities affect the continuance of social virtual worlds. To this end, we introduce the concept of perceived aggregate network exposure (PANE). We extend the original information systems (IS) continuance model with perceived enjoyment and position PANE as a moderator. We test the model with data collected from 2134 Finnish Habbo Hotel users and employ structural equation modelling in the analysis. The results demonstrate that PANE moderates the influence of motivational factors on continued use intention and satisfaction. 相似文献
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企业管理的国际化要求企业的管理与国际管理相接轨,做到科学有效,提高产品的竞争力。企业认证则为企业提供了国际先进的管理模式,二者结合,使企业在管理中不断发展和完善。 相似文献
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In this paper, we propose a new continuous self‐collision detection (CSCD) method for a deformable surface that interacts with a simple solid model. The method is developed based on the radial‐view‐based culling method. Our method is suitable for the deformable surface that has large contact region with the solid model. The deformable surface may consist of small round‐shaped holes. At the pre‐processing stage, the holes of the deformable surface are filled with ghost triangles so as to make the mesh of the deformable surface watertight. An observer primitive (i.e. a point or a line segment) is computed so that it lies inside the solid model. At the runtime stage, the orientations of triangles with respect to the observer primitive are evaluated. The collision status of the deformable surface is then determined. We evaluated our method for several animations including virtual garments. Experimental results show that our method improves the process of CSCD. 相似文献
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树立正确的营销意识,丰富营销方法,有利于供电企业更好地适应不断变化的市场,提供质量更高的市场服务。分析了供电企业电力营销中存在的问题,提出了具体的改革方法。 相似文献