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1.
Di(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in the manufacture of polyvinylchloride plastics and has been associated with concerns regarding male reproductive toxicity. In this study, we hypothesized that maternal exposure to DEHP induces transgenerational inheritance of adult-onset adverse reproductive outcomes through the male germline in the F1, F2, and F3 generations of male offspring. Pregnant rats were treated with 5 or 500 mg of DEHP/kg/day through gavage from gestation day 0 to birth. The offspring body weight, anogenital distance (AGD), anogenital index (AGI), sperm count, motility, and DNA fragmentation index (DFI) were measured for all generations. Methyl-CpG binding domain sequencing was performed to analyze sperm DNA methylation status in the F3. DEHP exposure at 500 mg/kg affected AGD, AGI, sperm count, mean DFI, and %DFI in the F1; AGD, sperm count, and mean DFI in the F2; and AGD, AGI, mean DFI, and %DFI in the F3. DEHP exposure at 5 mg/kg affected AGD, AGI, sperm count, and %DFI in the F1; sperm count in the F2; and AGD and AGI in F3. Compared with the control group, 15 and 45 differentially hypermethylated genes were identified in the groups administered 5 mg/kg and 500 mg/kg DEHP, respectively. Moreover, 130 and 6 differentially hypomethylated genes were observed in the groups administered 5 mg/kg and 500 mg/kg DEHP. Overall, these results demonstrated that prenatal exposure to DEHP caused transgenerational epigenetic effects, which may explain the observed phenotypic changes in the male reproductive system.  相似文献   
2.
A new graphical estimation technique is proposed for the determination of the kinetic parameters describing an autocatalytic reaction. A differential scanning calorimeter was used to monitor the reaction kinetics of an epoxy-based vinyl ester resin. The method utilizes information from a zero initial reaction rate, conversation at vitrification, the ratio of reaction rate constants under different isothermal conditions, and characteristics of the phenomenological kinetic model with assumptions being made about the overall reaction order. By fitting data to the integrated reaction rate equation with adjustments for the isothermal conditions, the kinetic parameters are estimated without using a linear or nonlinear regression method. Different kinetic parameters can be estimated from data before and after the gel point which was obtained from the relationship between the glass transition temperature and the degree of cure.  相似文献   
3.
在信息抽取领域,从非结构化文本中抽取实体关系是一项基础且重要的任务,且面临实体重叠和模型误差累积等挑战.本文以关系为导向,提出一种改进的实体关系联合抽取方法.该方法将实体关系抽取任务分为关系抽取与实体抽取两个子任务.在关系抽取任务上采用自注意力机制关注词与词之间的重要程度从而模拟实体信息,并使用平均池化来表征整个句子信息;在实体抽取任务上结合关系信息使用条件随机场识别该关系下的实体对.本模型不仅能够利用存在关系必定存在实体对的思想解决实体对重叠问题,还能够在训练过程中利用数据集中已知的关系使实体抽取模块不依赖于关系抽取模块的结果来训练,从而在训练阶段避免误差累积.最后,在WebNLG和NYT公开数据集上验证了该模型的有效性.  相似文献   
4.
为突破现有的X射线单晶衍射仪操作与控制系统上位机直接控制下位机模式,采用RabbitMQ企业级消息队列服务器作为控制通信服务器,并以此为基础搭建X射线单晶衍射仪远程控制系统的消息通信平台,实现对分布式各个子系统的远程操作与控制.本文介绍了针对远程控制系统的消息通信系统进行设计,初步测试表明在此通信系统上能够实现稳定精准的消息通信.  相似文献   
5.
运用业务流程重组的理论,以某集团企业为实例,论述了BPR在大型企业招标信息化建设中的重要作用,并从体制、制度、流程的重构几个层面,指导招标信息化建设。同时,也通过对招标信息化与业务流程重组实施相结合进行探讨,以期实现招标管理水平的有效提升。  相似文献   
6.
Water Resources Management - Effectively assessing crucial monitoring sites with suspended sediment concentration (SSC) is a vital challenge for achieving accurate prediction of sediment flux on...  相似文献   
7.
天然食品防腐剂按其来源不同可分为植物源天然防腐剂、动物源天然防腐剂和微生物源天然防腐剂。微生物源天然食品防腐剂由于其本身的优越性,越来越受到人们的青睐。本文主要介绍了微生物来源的天然食品防腐剂乳酸链球菌素、ε-聚赖氨酸、曲酸、溶菌酶和甲烷氧化菌素的最新研究进展,阐述了它们的理化性质、抑菌机制和应用现状,同时简单介绍了纳他霉素、抗菌肽、罗伊氏菌素和嗜杀酵母等微生物源天然食品防腐剂的应用现状,旨在为高效、无毒、天然食品防腐剂的研究提供参考。天然食品防腐剂最有发展的可能是将几种来源不同的天然食品防腐剂复配,或者将一种天然防腐剂与其他化学防腐剂复配使用,不仅可以减少防腐剂在食品中的用量,同时还可以使食品的杀菌或抑菌条件更加温和化,对人们的饮食健康非常有利。  相似文献   
8.
Electronic auction (e-auction), a major e-commerce model, has enjoyed rapid growth in many countries. This study develops a web assessment model (EAWAM) from a consumer perspective that suggests that user intention to continue using an e-auction website is based on users’ satisfaction with the e-auction website, which is determined by perceived e-auction user friendliness, functionality and interactivity. Web trustworthiness serves as an important antecedent to perceived e-auction user friendliness and functionality. To validate this model, a survey was conducted using 191 users of Taobao.com from 15 cities in China. Partial Least Square analysis results provided strong support for this model and also yielded important implications and suggestions for further research.  相似文献   
9.
The purpose of this experiment was to determine the antidiabetic and lipid-lowering effects of (−)-epicatechin-3-O-β-d-allopyranoside (BB) from the roots and stems of Davallia formosana in mice. Animal treatment was induced by high-fat diet (HFD) or low-fat diet (control diet, CD). After eight weeks of HFD or CD exposure, the HFD mice were treating with BB or rosiglitazone (Rosi) or fenofibrate (Feno) or water through gavage for another four weeks. However, at 12 weeks, the HFD-fed group had enhanced blood levels of glucose, triglyceride (TG), and insulin. BB treatment significantly decreased blood glucose, TG, and insulin levels. Moreover, visceral fat weights were enhanced in HFD-fed mice, accompanied by increased blood leptin concentrations and decreased adiponectin levels, which were reversed by treatment with BB. Muscular membrane protein levels of glucose transporter 4 (GLUT4) were reduced in HFD-fed mice and significantly enhanced upon administration of BB, Rosi, and Feno. Moreover, BB treatment markedly increased hepatic and skeletal muscular expression levels of phosphorylation of AMP-activated (adenosine monophosphate) protein kinase (phospho-AMPK). BB also decreased hepatic mRNA levels of phosphenolpyruvate carboxykinase (PEPCK), which are associated with a decrease in hepatic glucose production. BB-exerted hypotriglyceridemic activity may be partly associated with increased mRNA levels of peroxisome proliferator activated receptor α (PPARα), and with reduced hepatic glycerol-3-phosphate acyltransferase (GPAT) mRNA levels in the liver, which decreased triacylglycerol synthesis. Nevertheless, we demonstrated BB was a useful approach for the management of type 2 diabetes and dyslipidemia in this animal model.  相似文献   
10.
Methanobactin (Mb) is a copper-binding peptide that appears to function as an agent for copper sequestration and uptake in methanotrophs. Mb can also bind and reduce Au(III) to Au(0). In this paper, Au/Al2O3 catalysts prepared by a novel incipient wetness-Mb-mediated bioreduction method were used for glucose oxidation. The catalysts were characterized, and the analysis revealed that very small gold nanoparticles with a particle size <4 nm were prepared by the incipient wetness-Mb-mediated bioreduction method, even at 1.0% Au loading (w/w). The influence of Au loading, calcination temperature and calcination time on the specific activity of Au/Al2O3 catalysts was systematically investigated. Experimental results showed that decomposing the Mb molecules properly by calcinations can enhance the specific activity of Au/Al2O3 catalysts, though they acted as reductant and protective agents during the catalyst preparation. Au/Al2O3 catalysts synthesized by the method exhibited optimum specific activity under operational synthesis conditions of Au loading of 1.0 wt % and calcined at 450 °C for 2 h. The catalysts were reused eight times, without a significant decrease in specific activity. To our knowledge, this is the first attempt at the preparation of Au/Al2O3 catalysts by Mb-mediated in situ synthesis of gold nanoparticles.  相似文献   
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