A rapid X-ray spectrometric (XRS) method has been developed for the determination of the total glucosinolate content of oilseed rape and other Brassica oilseeds. The method is based on analysis for fully oxidised sulphur (S6+), which includes half the sulphur (S) in the glucosinolate molecule, and the S in sulphate. Results are highly correlated with glucosinolate content determined by glucose release, a standard method widely used in Australia. The relationship is total glucosinolates = (23·97 S6+ -9·43) r2 = 0·987, where the glucosinolate content is expressed as μmol g?1 and the S6+ content in mg g?1. The relationship is applicable to seed of any glucosinolate content and to meal, and is unaffected by changes in protein sulphur content. The correlation of glucosinolates with S6+ is shown to be closer than the correlation with total S. The latter correlation forms the basis of the existing XRS method, used within the European Community in recent years. The advantages of S6+ derive from the linearity of the regression and the elimination of errors caused by variation in protein content. The method should be valuable to the Australian oilseed industry because it allows the rapid screening of breeding lines to ensure low glucosinolate content and the assessment of deliveries for crushing and of meal. 相似文献
A vertical polyacrylamide — SDS electrophoretic technique, including whole protein extraction and staining steps, was improved with a view to developing it for routine laboratory use with single barley kernels. The pattern consisted of 4 zones: A (albumins-globulins). B and C (hordeins) and D (possibly glutelins) displaying unequal varietal polymorphisms (1, 13, 13 and 4 types respectively). 28% of the barley samples (77 varieties), including most cultivars grown in France, could be unambiguously identified from qualitative differences only, in the B, C and D zones. Adding three other characteristics (hairs and furrow hairiness, peroxidase, zymogram, esterase zymogram), as many as 78% of the varieties could be identified, the other 22% consisting of very closely related barleys. After slight modification of protein extraction conditions, the same methods could be used with malt, based on the same electrophoretic types. A graphic tablet connected to a microcomputer was used for automatic acquisitions of records and comparisons of electrophoretic data. 相似文献
Twenty flours from 16 different barley varieties cultivated in 1990 and 1992, and a Swedish reference flour, were fermented by Lactobacillus plantarum A1 to sour doughs. Barley breads (40% barley/60% wheatflour) from each flour type were baked with and without an admixture of barley sour dough in order to investigate how the sour dough admixture would affect the baking properties. A trained panel carried out sensory evaluation by conventional profiling on breads made from three of the barley varieties and the Swedish reference flour, made with and without sour dough admixture.
The barley varieties influenced both the sour dough properties and the properties of the barley bread. The pH of bread with sour dough ranged from 4.6 to 4.8 as compared to 5.4 to 5.6 in bread without sour dough. The acidity of the breads with sour dough ranged from 4.1 to 5.0 ml NaOH/ 10 g bread crumb as compared to 2.4 to 3.6 in breads without sour dough. In 14 of the twenty bread types an addition of sour dough lowered the bread volume. Breads with a sour dough admixture scored higher for total taste and acidulous taste than breads without sour dough. The β-glucan content of the flours had no significant influence on the sour dough or the sensory characteristics of the bread, except for the breadcrumb colour. 相似文献