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1.
Transmission electron microscopy (TEM) is an important analysis technique to visualize (bio)macromolecules and their assemblies, including collagen fibers. Many protocols for TEM sample preparation of collagen involve one or more washing steps to remove excess salts from the dispersion that could hamper analysis when dried on a TEM grid. Such protocols are not standardized and washing times as well as washing solvents vary from procedure to procedure, with each research group typically having their own protocol. Here, we investigate the influence of washing with water, ethanol, but also methanol and 2-propanol, for both mineralized and unmineralized collagen samples via a protocol based on centrifugation. Washing with water maintains the hydrated collagen structure and the characteristic banding pattern can be clearly observed. Conversely, washing with ethanol results in dehydration of the fibrils, often leading to aggregation of the fibers and a less obvious banding pattern, already within 1 min of ethanol exposure. As we show, this process is fully reversible. Similar observations were made for methanol and propanol. Based on these results, a standardized washing protocol for collagenous samples is proposed.  相似文献   
2.
In actual engineering scenarios, limited fault data leads to insufficient model training and over-fitting, which negatively affects the diagnostic performance of intelligent diagnostic models. To solve the problem, this paper proposes a variational information constrained generative adversarial network (VICGAN) for effective machine fault diagnosis. Firstly, by incorporating the encoder into the discriminator to map the deep features, an improved generative adversarial network with stronger data synthesis capability is established. Secondly, to promote the stable training of the model and guarantee better convergence, a variational information constraint technique is utilized, which constrains the input signals and deep features of the discriminator using the information bottleneck method. In addition, a representation matching module is added to impose restrictions on the generator, avoiding the mode collapse problem and boosting the sample diversity. Two rolling bearing datasets are utilized to verify the effectiveness and stability of the presented network, which demonstrates that the presented network has an admirable ability in processing fault diagnosis with few samples, and performs better than state-of-the-art approaches.  相似文献   
3.
围绕人工智能与智慧海洋建设这条主线,论述人工智能、智慧海洋的概念,列举当前较为成熟的人工智能与海洋科技在海洋观测方面融合的切入点,初步展现一种海洋技术与装备智能化的发展路径,提出加快人工智能技术向智慧海洋建设赋能的几点建议。  相似文献   
4.
A new method for the polygonal approximation is presented. The method is based on the search for break points through a context-free grammar, that accepts digital straight segments with loss of information, as well as the decrease in the error committed employing the comparison of a tolerable error. We present an application of our method to different sets of objects widely used, as well as a comparison of our results with the best results reported in the literature, proving that our method achieves better values of error criteria. Besides, a new way to find polygonal approximations, with context-free grammars to recognize digital straight segments without loss of pixels, it is also addressed.  相似文献   
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6.
When five axis CNC machine tools follow series linear toolpath segments, the drives experience velocity, acceleration and jerk discontinuities at the block transition points. The discontinuities result in fluctuations on machine tool motions which lead to poor surface quality. This paper proposes to insert quintic and septic micro-splines for the tool tip and tool-orientation, respectively, at the adjacent linear toolpath segments. Optimal control points are calculated for position and orientation splines to achieve C3 continuity at the junctions while respecting user-defined tolerance limits. The geometrically smoothed corners are traveled at a smoothly varying feed with cubic acceleration trajectory profile. The proposed method is experimentally demonstrated to show improvements in motion smoothness and tracking accuracy in five-axis machining of free-form surfaces found in dies, molds and aerospace parts.  相似文献   
7.
We explore a truncation error criterion to steer adaptive step length refinement and coarsening in incremental-iterative path following procedures, applied to problems in large-deformation structural mechanics. Elaborating on ideas proposed by Bergan and collaborators in the 1970s, we first describe an easily computable scalar stiffness parameter whose sign and rate of change provide reliable information on the local behavior and complexity of the equilibrium path. We then derive a simple scaling law that adaptively adjusts the length of the next step based on the rate of change of the stiffness parameter at previous points on the path. We show that this scaling is equivalent to keeping a local truncation error constant in each step. We demonstrate with numerical examples that our adaptive method follows a path with a significantly reduced number of points compared to an analysis with uniform step length of the same fidelity level. A comparison with Abaqus illustrates that the truncation error criterion effectively concentrates points around the smallest-scale features of the path, which is generally not possible with automatic incrementation solely based on local convergence properties.  相似文献   
8.
为了实现大口径光学元件的安全装夹、转运,通过光学元件开槽与不开槽两种装夹方式的分析,得出开槽夹紧转运方式将带来微裂纹、应力集中、成本高等缺陷,提出了利用摩擦力克服光学零件的重力和惯性力的低应力装夹转运方案。通过对光学元件低应力夹紧结构设计,并利用有限元分析方法,得到不开槽装夹方式下,光学元件的最大主应力为1.11 MPa,最大切应力为0.73 MPa,远低于光学元件破坏的强度极限,且受力均匀,无应力集中现象。  相似文献   
9.
随着互联网的高速发展,引发了网络流量、电信骨干网流量急速增长,使得网络容量的提升迫在眉睫。目前,100G 系统已经商用,超100G系统能够更有效地解决流量和网络带宽持续增长带来的压力,本文主要介绍新型单模光纤的特性以及探讨基于新型单模光纤的传输技术。  相似文献   
10.
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